Anti-GSTM3 antibody
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Rabbit Polyclonal GSTM3 antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human GSTM3 aa 150 to C-terminus.
View Alternative Names
GST5, GSTM3, Glutathione S-transferase Mu 3, GST class-mu 3, GSTM3-3, hGSTM3-3
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSTM3 antibody (AB272613)
Paraffin-embedded formalin-fixed Human testis tissue stained for GSTM3 using ab272613 at 1/500 in immunohistochemical analysis.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSTM3 antibody (AB272613)
Paraffin-embedded formalin-fixed Human lymph tissue stained for GSTM3 using ab272613 at 1/500 in immunohistochemical analysis. Low expression as expected.
- WB
Unknown
Western blot - Anti-GSTM3 antibody (AB272613)
Lane 1:
Western blot - Anti-GSTM3 antibody (ab272613)
Lane 2:
Western blot - Anti-GSTM3 antibody (ab272613) at 0.4 µg/mL
Lane 1:
Molecular weight ladder
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Human cell line RT4
Predicted band size: 27 kDa
false
- WB
Lab
Western blot - Anti-GSTM3 antibody (AB272613)
Western blot : Anti-GSTM3 antibody (ab272613) staining at 0.4 ug/ml, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab272613 was shown to bind specifically to GSTM3. A band was observed at 25 kDa in wild-type A549 cell lysates with no signal observed at this size in GSTM3 knockout cell line. To generate this image, wild-type and GSTM3 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes:
Western blot - Anti-GSTM3 antibody (ab272613) at 0.4 µg/mL
Lane 1:
Wild-type A549 cell lysate at 20 µg
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GSTM3 knockout A549 cell lysate at 20 µg
Lane 3:
MCF7 cell lysate at 20 µg
Lane 4:
HCT 116 cell lysate at 20 µg
Secondary
Lanes 1 - 4:
Goat anti-Rabbit IgG H&L 800CW at 1/20000 dilution
Lanes 1 - 4:
Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Observed band size: 25 kDa
false
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Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
GSTM3 facilitates the neutralization of reactive oxygen species protecting cells from oxidative damage. This protein interacts with intracellular substrates engaging in phase II metabolic reactions. While GSTM3 does not form larger multimeric complexes it partners with glutathione to stabilize and detoxify harmful compounds. This role highlights GSTM3’s importance within cellular defense mechanisms.
Pathways
GSTM3 participates in the xenobiotic metabolism and the glutathione pathway. These pathways involve the biotransformation of toxic substances where GSTM3 works in tandem with related proteins like CYP450 enzymes. GSTM3's activity in the glutathione pathway interconnects with the regulation of oxidative stress highlighting its part in cellular resilience against environmental challenges.
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