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AB154018

Anti-GSTM5 antibody

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(1 Publication)

Rabbit Polyclonal GSTM5 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human GSTM5 aa 1 to C-terminus.

View Alternative Names

Glutathione S-transferase Mu 5, GST class-mu 5, GSTM5-5, GSTM5

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSTM5 antibody (AB154018)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSTM5 antibody (AB154018)

Immunohistochemical analysis of paraffin-embedded Human SG xenograft tissue labeling GSTM5 with ab154018 at 1/500 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-GSTM5 antibody (AB154018)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-GSTM5 antibody (AB154018)

Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling GSTM5 with ab154018 at 1/200 dilution. Lower image is merged with DNA probe.

Western blot - Anti-GSTM5 antibody (AB154018)
  • WB

Unknown

Western blot - Anti-GSTM5 antibody (AB154018)

12% SDS PAGE

All lanes:

Western blot - Anti-GSTM5 antibody (ab154018) at 1/10000 dilution

All lanes:

Rat testis whole cell lysate at 50 µg

Predicted band size: 26 kDa

false

Western blot - Anti-GSTM5 antibody (AB154018)
  • WB

Unknown

Western blot - Anti-GSTM5 antibody (AB154018)

12% SDS PAGE

All lanes:

Western blot - Anti-GSTM5 antibody (ab154018) at 1/10000 dilution

All lanes:

Mouse testis whole cell lysate at 50 µg

Predicted band size: 26 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human GSTM5 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P46439

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GSTM5 also known as glutathione S-transferase Mu 5 is an enzyme that plays a pivotal role in detoxification processes. GSTM5 has a molecular mass of roughly 26 kDa. This protein belongs to the glutathione S-transferase family which are important for catalyzing the conjugation of glutathione to various substrates. GSTM5 is mainly expressed in tissues like the liver and lungs where detoxification is essential for maintaining cellular health.
Biological function summary

GSTM5 enzymes help in neutralizing toxins and products of oxidative stress by facilitating the conjugation of reduced glutathione to electrophilic compounds. GSTM5 does not function in isolation; it operates as part of the larger GST family that includes various isoforms like GSTM1 and GSTM3 sharing the responsibility of detoxifying harmful chemicals. Through its enzyme activity GSTM5 supports the protection of cells from toxic damage contributing to cellular antioxidant defense.

Pathways

GSTM5 activity contributes to the metabolic pathway involving the detoxification of xenobiotics playing a role in phase II metabolism. It is strongly connected to the glutathione conjugation pathway helping in the clearance of lipid peroxidation products. GSTM5 interacts closely with other proteins in the GST family including GSTP1 pinpointing its role in maintaining cellular redox balance and aiding in the conversion of harmful substances into more water-soluble products for excretion.

GSTM5 is associated with cancer and neurodegenerative diseases. Variations in GSTM5 expression have been linked to increased risk in the development of certain cancers given its role in detoxifying carcinogens. Moreover GSTM5 may influence the progression of neurodegenerative disorders by modulating oxidative stress responses a process where its counterpart GSTP1 also plays a significant part. Understanding GSTM5’s function can offer insights into potential therapeutic approaches for these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
See full target information GSTM5
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

PloS one 13:e0190698 PubMed29470513

2018

Offspring sex impacts DNA methylation and gene expression in placentae from women with diabetes during pregnancy.

Applications

Unspecified application

Species

Unspecified reactive species

Jacqueline Alexander,April M Teague,Jing Chen,Christopher E Aston,Yuet-Kin Leung,Steven Chernausek,Rebecca A Simmons,Sara E Pinney
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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