AB199337

Anti-GSTT1 antibody [EPR19283]

RabMAb
Recombinant
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(2 Publications)

Rabbit Recombinant Monoclonal GSTT1 antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

View Alternative Names

Glutathione S-transferase theta-1, GST class-theta-1, Glutathione transferase T1-1, GSTT1

4 Images

Supplier Data

Western blot - Anti-GSTT1 antibody [EPR19283] (AB199337)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1 and 2 : 3 minutes; Lane 3 : 5 seconds; Lane 4 : 30 seconds.

All lanes:

Western blot - Anti-GSTT1 antibody [EPR19283] (ab199337) at 1/1000 dilution

Lane 1:

Mouse lung lysate at 20 µg

Lane 2:

Rat lung lysate at 20 µg

Lane 3:

Mouse liver lysate at 20 µg

Lane 4:

Rat liver lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 27 kDa

Observed band size: 24 kDa

false

Supplier Data

Immunoprecipitation - Anti-GSTT1 antibody [EPR19283] (AB199337)

GSTT1 was immunoprecipitated from 1mg of HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with ab199337 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab199337 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1 : HepG2 whole cell lysate, 10μg (Input).
Lane 2 : ab199337 IP in HepG2 whole cell lysate.
Lane 3 : Rabbit IgG,monoclonal [EPR25A] -Isotype Control (ab172730) instead of ab199337 in HepG2 whole cell lysate.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 3 minutes.

All lanes:

Immunoprecipitation - Anti-GSTT1 antibody [EPR19283] (ab199337)

Predicted band size: 27 kDa

false

Supplier Data

Western blot - Anti-GSTT1 antibody [EPR19283] (AB199337)

Blocking/Dilution buffer : 5% NFDM/TBST.

Jurkat doesn't express GSTT1. The expression profile observed is consistent with what has been described in the literature (PMID : 24586676).

All lanes:

Western blot - Anti-GSTT1 antibody [EPR19283] (ab199337) at 1/1000 dilution

Lane 1:

Human fetal kidney lysate at 20 µg

Lane 2:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

Lane 3:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 27 kDa

Observed band size: 24 kDa

false

Exposure time: 30s

Supplier Data

Western blot - Anti-GSTT1 antibody [EPR19283] (AB199337)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-GSTT1 antibody [EPR19283] (ab199337) at 1/1000 dilution

All lanes:

Human fetal liver tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 27 kDa

Observed band size: 24 kDa

false

Exposure time: 3min

Carrier free

Anti-GSTT1 antibody [EPR19283] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19283

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/40", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GSTT1 or Glutathione S-transferase theta-1 functions mechanically as an enzyme involved in detoxification. It catalyzes the conjugation of glutathione to a wide array of substrates assisting in the metabolism and elimination of toxic compounds. This protein is part of the glutathione S-transferase family and usually presents as a 26 kDa unit. It expresses in the liver kidney and other tissues indicating a broad role in processing harmful substances.

Biological function summary

GSTT1 participates in cellular defense mechanisms by protecting cells against oxidative stress and free radicals. It does not form part of a larger protein complex but its activity can affect other detoxification enzymes in a cascade of reactions. Its function helps maintain cellular homeostasis and enhance the organism's ability to handle chemical stressors. The enzyme's role in metabolizing endogenous and exogenous compounds implicates it in basic cellular defense.

Pathways

GSTT1 influences the xenobiotic metabolism pathway and glutathione metabolism pathway. It interacts with proteins like CYP450 further facilitating the conversion of lipophilic substances into more water-soluble forms for excretion. These interactions play an important part in maintaining balance during detoxification processes. Such roles ensure the detoxification of a wide variety of harmful substances that could otherwise accumulate to toxic levels.

Mutations or deficiency of GSTT1 relate to an increased susceptibility to certain cancers and asthma. Such conditions arise due to compromised detoxification capability allowing potentially harmful compounds to damage cells and tissues. GSTT1 connects to proteins like GSTM1 another member of the glutathione S-transferase family wherein polymorphisms can compound susceptibility to disease emphasizing the enzyme's importance in predisposition to disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Acts on 1,2-epoxy-3-(4-nitrophenoxy)propane, phenethylisothiocyanate 4-nitrobenzyl chloride and 4-nitrophenethyl bromide. Displays glutathione peroxidase activity with cumene hydroperoxide.

See full target information Glutathione S-transferase theta-1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Particle and fibre toxicology 17:22 PubMed32503629

2020

Caloric restriction attenuates C57BL/6 J mouse lung injury and extra-pulmonary toxicity induced by real ambient particulate matter exposure.

Applications

Unspecified application

Species

Unspecified reactive species

Daochuan Li,Shen Chen,Qiong Li,Liping Chen,Haiyan Zhang,Huiyao Li,Dianke Yu,Rong Zhang,Yujie Niu,Shaoyou Lu,Lizhu Ye,Xiaowen Zeng,Guanghui Dong,Rui Chen,Michael Aschner,Yuxin Zheng,Wen Chen

The Journal of clinical investigation 130:2630-2643 PubMed32045382

2020

Erythrocyte-derived microvesicles induce arterial spasms in JAK2V617F myeloproliferative neoplasm.

Applications

Unspecified application

Species

Unspecified reactive species

Johanne Poisson,Marion Tanguy,Hortense Davy,Fatoumata Camara,Marie-Belle El Mdawar,Marouane Kheloufi,Tracy Dagher,Cécile Devue,Juliette Lasselin,Aurélie Plessier,Salma Merchant,Olivier Blanc-Brude,Michèle Souyri,Nathalie Mougenot,Florent Dingli,Damarys Loew,Stephane N Hatem,Chloé James,Jean-Luc Villeval,Chantal M Boulanger,Pierre-Emmanuel Rautou

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GSTT1 antibody [EPR19283]

Western blot - Anti-GSTT1 antibody [EPR19283] (AB199337)

Immunoprecipitation - Anti-GSTT1 antibody [EPR19283] (AB199337)

Western blot - Anti-GSTT1 antibody [EPR19283] (AB199337)

Western blot - Anti-GSTT1 antibody [EPR19283] (AB199337)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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