Anti-GSTT1 antibody [EPR19283] - BSA and Azide free

• WB

Supplier Data

Western blot - Anti-GSTT1 antibody [EPR19283] - BSA and Azide free (AB251268)

This data was developed using ab199337, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : Lanes 1 and 2 : 3 minutes; Lane 3 : 5 seconds; Lane 4 : 30 seconds.

All lanes:

Western blot - Anti-GSTT1 antibody [EPR19283] (<a href='/en-us/products/primary-antibodies/gstt1-antibody-epr19283-ab199337'>ab199337</a>) at 1/1000 dilution

Lane 1:

Mouse lung lysate at 20 µg

Lane 2:

Rat lung lysate at 20 µg

Lane 3:

Mouse liver lysate at 20 µg

Lane 4:

Rat liver lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 27 kDa

Observed band size: 24 kDa

false

• IP

Supplier Data

Immunoprecipitation - Anti-GSTT1 antibody [EPR19283] - BSA and Azide free (AB251268)

This data was developed using ab199337, the same antibody clone in a different buffer formulation.

GSTT1 was immunoprecipitated from 1mg of HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with ab199337 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab199337 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1 : HepG2 whole cell lysate, 10μg (Input).
Lane 2 : ab199337 IP in HepG2 whole cell lysate.
Lane 3 : Rabbit IgG,monoclonal [EPR25A] -Isotype Control (ab172730) instead of ab199337 in HepG2 whole cell lysate.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 3 minutes.

All lanes:

Immunoprecipitation - Anti-GSTT1 antibody [EPR19283] (<a href='/en-us/products/primary-antibodies/gstt1-antibody-epr19283-ab199337'>ab199337</a>)

Predicted band size: 27 kDa

false

• WB

Supplier Data

Western blot - Anti-GSTT1 antibody [EPR19283] - BSA and Azide free (AB251268)

This data was developed using ab199337, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Jurkat doesn't express GSTT1. The expression profile observed is consistent with what has been described in the literature (PMID : 24586676).

All lanes:

Western blot - Anti-GSTT1 antibody [EPR19283] (<a href='/en-us/products/primary-antibodies/gstt1-antibody-epr19283-ab199337'>ab199337</a>) at 1/1000 dilution

Lane 1:

Human fetal kidney lysate at 20 µg

Lane 2:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

Lane 3:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 27 kDa

Observed band size: 24 kDa

false

Exposure time: 30s

• WB

Supplier Data

Western blot - Anti-GSTT1 antibody [EPR19283] - BSA and Azide free (AB251268)

This data was developed using ab199337, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-GSTT1 antibody [EPR19283] (<a href='/en-us/products/primary-antibodies/gstt1-antibody-epr19283-ab199337'>ab199337</a>) at 1/1000 dilution

All lanes:

Human fetal liver tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 27 kDa

Observed band size: 24 kDa

false

Exposure time: 3min