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AB176336

Anti-GSTT2 antibody [EPR8136(2)]

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(1 Publication)

Rabbit Recombinant Monoclonal GSTT2 antibody. Suitable for WB and reacts with Human, Mouse samples. Cited in 1 publication.

View Alternative Names

GSTT2, GSTT2B, Glutathione S-transferase theta-2B, Glutathione S-transferase theta-2, GST class-theta-2

3 Images
Western blot - Anti-GSTT2 antibody [EPR8136(2)] (AB176336)
  • WB

Supplier Data

Western blot - Anti-GSTT2 antibody [EPR8136(2)] (AB176336)

Lanes 1-4 : Merged signal (red and green). Green - ab176336 observed at 28 kDa. Red - loading control, ab7291 observed at 50 kDa.

ab176336 Anti-GSTT2 antibody [EPR8136(2)] was shown to specifically react with GSTT2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264703 (knockout cell lysate ab258443) was used. Wild-type and GSTT2 knockout samples were subjected to SDS-PAGE. ab176336 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-GSTT2 antibody [EPR8136(2)] (ab176336) at 1/500 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

GSTT2B knockout HeLa cell lysate at 20 µg

Lane 3:

Human lung tissue lysate at 20 µg

Lane 4:

A549 cell lysate at 20 µg

Predicted band size: 28 kDa

Observed band size: 28 kDa

false

Western blot - Anti-GSTT2 antibody [EPR8136(2)] (AB176336)
  • WB

Lab

Western blot - Anti-GSTT2 antibody [EPR8136(2)] (AB176336)

Lanes 1-4 : Merged signal (red and green). Green - ab176336 observed at 28 kDa. Red - loading control, ab7291 observed at 50 kDa.

ab176336 Anti-GSTT2 antibody [EPR8136(2)] was shown to specifically react with GSTT2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264703 (knockout cell lysate ab258443) was used. Wild-type and GSTT2 knockout samples were subjected to SDS-PAGE. ab176336 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-GSTT2 antibody [EPR8136(2)] (ab176336) at 1/500 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

GSTT2B knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human GSTT2B (GSTT2) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-gstt2b-gstt2-knockout-hela-cell-line-ab264703'>ab264703</a>)

Lane 3:

Human lung tissue lysate at 20 µg

Lane 4:

A549 cell lysate at 20 µg

Predicted band size: 28 kDa

Observed band size: 28 kDa

false

Western blot - Anti-GSTT2 antibody [EPR8136(2)] (AB176336)
  • WB

Supplier Data

Western blot - Anti-GSTT2 antibody [EPR8136(2)] (AB176336)

All lanes:

Western blot - Anti-GSTT2 antibody [EPR8136(2)] (ab176336) at 1/1000 dilution

Lane 1:

Human prostate lysate at 10 µg

Lane 2:

Mouse lung lysate at 10 µg

Lane 3:

Fetal lung lysate at 10 µg

Predicted band size: 28 kDa

false

  • Carrier free

    Anti-GSTT2 antibody [EPR8136(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8136(2)

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GSTT2 also known as Glutathione S-transferase Theta-2 belongs to the GST superfamily of enzymes. This protein has an approximate molecular mass of 27 kDa. GSTT2 is involved in the conjugation of reduced glutathione to various electrophilic compounds facilitating the detoxification process. It is expressed in several tissues with notable presence in the liver kidney and testis. GSTT2 plays a role in processing many xenobiotic substances enhancing cell resistance to oxidative stress.
Biological function summary

GSTT2 facilitates the detoxification process by catalyzing reactions that neutralize harmful compounds. It catalyzes the conjugation of glutathione to form less reactive electrophilic or hydrophobic substances. GSTT2 does not function as part of a larger complex within cells but has an important role in maintaining the redox balance. The enzyme contributes to cellular defense mechanisms ensuring proper cellular functions in stressful environments.

Pathways

The activity of GSTT2 has connections to the glutathione metabolism and drug metabolism pathways. These pathways are important for neutralizing endogenous and exogenous toxins. In the glutathione metabolism pathway GSTT2 works in conjunction with other GST enzymes like GSTP1 and GSTA1 to manage oxidative stress. The protein indirectly supports cellular detoxification processes influencing how effectively a cell can endure and adapt to toxic insults.

GSTT2's role in detoxification links it to conditions such as cancer and liver diseases. Altered GSTT2 expression has associations with the development of hepatocellular carcinoma. The protein interacts with related GST family members like GSTM1 and GSTP1 which play roles in modulating cancer susceptibility. Due to its role in processing carcinogens and protecting tissues from oxidative damage GSTT2 presents as a noteworthy protein in understanding disease mechanisms and potential therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles (PubMed : 1417752). Has a sulfatase activity (PubMed : 1417752).
See full target information GSTT2B
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer biology & therapy 24:2216041 PubMed37287122

2023

CSTF2T facilitates pancreatic adenocarcinoma growth and metastasis by elevating H3K4Me1 methylation of CALB2 via ASH2L.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Liu,Dongwei Hu,Jinjie Li,Qigang Xu,Zongjing Chen,Bo Xu
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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