AB187143

Anti-GTF2E2 antibody [EPR14929]

RabMAb
Recombinant
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(4 Publications)

Rabbit Recombinant Monoclonal GTF2E2 antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 4 publications.

View Alternative Names

TF2E2, GTF2E2, Transcription initiation factor IIE subunit beta, TFIIE-beta, General transcription factor IIE subunit 2

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GTF2E2 antibody [EPR14929] (AB187143)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GTF2E2 antibody [EPR14929] (AB187143)

Immunohistochemical analysis of paraffin embedded Human cervix carcinoma tissue labeling GTF2E2 with ab187143 at 1/250 with prediluted HRP Polymer for Rabbit IgG as a secondary antibody.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-GTF2E2 antibody [EPR14929] (AB187143)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GTF2E2 antibody [EPR14929] (AB187143)

Immunofluorescent analysis of 293T cells labeling GTF2E2 with ab187143 at 1/250 and DAPI staining (blue).

Supplier Data

Immunoprecipitation - Anti-GTF2E2 antibody [EPR14929] (AB187143)

Immunoprecipitation. ab187143 at 1/50 labeling GTF2E2 immunoprecipitated from Jurkat cell lysate using ab187143 at 1/30.

All lanes:

Immunoprecipitation - Anti-GTF2E2 antibody [EPR14929] (ab187143)

Predicted band size: 33 kDa

Observed band size: 33 kDa

false

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GTF2E2 antibody [EPR14929] (AB187143)

Immunohistochemical analysis of paraffin embedded Mouse liver tissue labeling GTF2E2 with ab187143 at 1/250 with a prediluted HRP Polymer for Rabbit IgG as a secondary antibody.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-GTF2E2 antibody [EPR14929] (AB187143)

All lanes:

Western blot - Anti-GTF2E2 antibody [EPR14929] (ab187143) at 1/10000 dilution

Lane 1:

K562 lysate at 20 µg

Lane 2:

Jurkat lysate at 20 µg

Lane 3:

293T lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 33 kDa

Observed band size: 33 kDa

false

Supplier Data

Western blot - Anti-GTF2E2 antibody [EPR14929] (AB187143)

All lanes:

Western blot - Anti-GTF2E2 antibody [EPR14929] (ab187143) at 1/1000 dilution

Lanes 1 and 3:

Mouse heart tissue lysate at 10 µg

Lane 2:

Rat brain tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 33 kDa

Observed band size: 33 kDa

false

Carrier free

Anti-GTF2E2 antibody [EPR14929] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14929

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF, IHC-P, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30 - 1/50", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target GTF2E2 also known as General Transcription Factor IIE Subunit 2 participates in the initiation of transcription by RNA polymerase II. It plays an important role in the formation of the transcription initiation complex. The protein has a molecular mass of approximately 32 kDa. GTF2E2 is expressed in various tissues with significant expression detected in the human heart and skeletal muscle.

Biological function summary

GTF2E2 interacts with other general transcription factors to promote the assembly of the pre-initiation complex. It forms a part of the TFIIE complex with another subunit TFIIE α. This complex is significant for transcription initiation as it helps in opening the DNA double helix. Its activity influences gene expression regulation and is important for directing RNA polymerase II to specific promoter sites.

Pathways

The protein is involved in the RNA polymerase II transcription initiation pathway a critical step for mRNA synthesis. Additionally it affects the regulation of the p53 signaling pathway often interacting with proteins like TFIIH and TBP. The association with these pathways highlights its role in maintaining the gene expression safety and responding to cellular stress signals.

Altered expression of GTF2E2 correlates with cancer and developmental disorders. Its dysfunction might lead to malformed transcription initiation influencing oncogenic pathways. Mutations or misregulation can affect the stability of the transcription machinery potentially linking it to the BRCA1 protein in breast cancer further highlighting the need for precise transcription control.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.

See full target information GTF2E2

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cells 10: PubMed34944018

2021

Mayaro Virus Non-Structural Protein 2 Circumvents the Induction of Interferon in Part by Depleting Host Transcription Initiation Factor IIE Subunit 2.

Applications

Unspecified application

Species

Unspecified reactive species

Ray Ishida,Jamie Cole,Joaquin Lopez-Orozco,Nawell Fayad,Alberto Felix-Lopez,Mohamed Elaish,Shu Yue Luo,Olivier Julien,Anil Kumar,Tom C Hobman

Oncogene 41:782-796 PubMed34853466

2021

GTF2E2 is a novel biomarker for recurrence after surgery and promotes progression of esophageal squamous cell carcinoma via miR-139-5p/GTF2E2/FUS axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yujie Zhang,Yuxin Zhang,Bo Ai,Juejun Gong,Yichen Li,Shiying Yu,Xiuyu Cai,Li Zhang

Cell systems 5:386-398.e4 PubMed29032074

2017

Widespread Post-transcriptional Attenuation of Genomic Copy-Number Variation in Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Emanuel Gonçalves,Athanassios Fragoulis,Luz Garcia-Alonso,Thorsten Cramer,Julio Saez-Rodriguez,Pedro Beltrao

Human molecular genetics 26:4689-4698 PubMed28973399

2017

Trichothiodystrophy causative TFIIEβ mutation affects transcription in highly differentiated tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Arjan F Theil,Imke K Mandemaker,Emile van den Akker,Sigrid M A Swagemakers,Anja Raams,Tatjana Wüst,Jurgen A Marteijn,Jacques C Giltay,Richard M Colombijn,Ute Moog,Urania Kotzaeridou,Mehrnaz Ghazvini,Marieke von Lindern,Jan H J Hoeijmakers,Nicolaas G J Jaspers,Peter J van der Spek,Wim Vermeulen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GTF2E2 antibody [EPR14929]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GTF2E2 antibody [EPR14929] (AB187143)

Immunocytochemistry/ Immunofluorescence - Anti-GTF2E2 antibody [EPR14929] (AB187143)

Immunoprecipitation - Anti-GTF2E2 antibody [EPR14929] (AB187143)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GTF2E2 antibody [EPR14929] (AB187143)

Western blot - Anti-GTF2E2 antibody [EPR14929] (AB187143)

Western blot - Anti-GTF2E2 antibody [EPR14929] (AB187143)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise