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AB128945

Anti-GTF2F2 antibody [EPR7584]

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(1 Publication)

Rabbit Recombinant Monoclonal GTF2F2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

RAP30, GTF2F2, General transcription factor IIF subunit 2, General transcription factor IIF 30 kDa subunit, Transcription initiation factor IIF subunit beta, Transcription initiation factor RAP30, TFIIF-beta

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GTF2F2 antibody [EPR7584] (AB128945)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GTF2F2 antibody [EPR7584] (AB128945)

ab128945 at a 1/50 dilution staining GTF2F2 in paraffin-embedded Human cervical carcinoma tissue by immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GTF2F2 antibody [EPR7584] (AB128945)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GTF2F2 antibody [EPR7584] (AB128945)

ab128945 at a 1/50 dilution staining GTF2F2 in paraffin-embedded Human colon tissue by immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-GTF2F2 antibody [EPR7584] (AB128945)
  • WB

Unknown

Western blot - Anti-GTF2F2 antibody [EPR7584] (AB128945)

All lanes:

Western blot - Anti-GTF2F2 antibody [EPR7584] (ab128945) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 28 kDa

Observed band size: 30 kDa

false

  • Carrier free

    Anti-GTF2F2 antibody [EPR7584] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7584

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GTF2F2 also known as RAP74 forms a critical component of the general transcription factor IIF. It possesses an approximate mass of 74 kDa. This protein ubiquitously expresses in human cells acting as a cofactor within RNA polymerase II transcription machinery. GTF2F2 binds with RNA polymerase II influencing transcription initiation by stabilizing the transcription pre-initiation complex.
Biological function summary

GTF2F2 plays a significant role in enhancing the process of transcription elongation. It joins the multi-protein transcription factor IIF complex collaborating with subunits such as TFIIF. GTF2F2's activity is necessary for promoter clearance and efficient RNA synthesis from DNA templates bridging interactions between transcription factors and the polymerase.

Pathways

Involvement of GTF2F2 is important in the transcriptional regulation pathway. It coordinates with RNA polymerase II and influences the transcription cycle particularly pathways related to transcription elongation. GTF2F2 shares interactions with other transcription-associated proteins including TFIIB and TFIIH which further integrate into these pathways to optimize transcription efficiency and fidelity.

Studies link GTF2F2 to neurological disorders including mental retardation. The protein's disruption might alter transcriptional regulation leading to developmental anomalies. In cancer it holds a connection with proteins like p53 where changes in transcription factor activity influenced by GTF2F2 can affect cancer progression or response to treatment through disruption of proper gene expression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB.
See full target information GTF2F2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancers 15: PubMed36612143

2022

Activin B and Activin C Have Opposing Effects on Prostate Cancer Progression and Cell Growth.

Applications

Unspecified application

Species

Unspecified reactive species

Karen L Reader,Simon John-McHaffie,Sylvia Zellhuber-McMillan,Tim Jowett,David G Mottershead,Heather E Cunliffe,Elspeth J Gold
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com