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AB204168

Anti-GTF2H1 antibody

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(1 Publication)

Rabbit Polyclonal GTF2H1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human GTF2H1 aa 50-200.
4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GTF2H1 antibody (AB204168)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GTF2H1 antibody (AB204168)

Immunohistochemical analysis of human skeletal muscle tissue labeling GTF2H1 (low expression) with ab204168 at a 1/50 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-GTF2H1 antibody (AB204168)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GTF2H1 antibody (AB204168)

Immunocytochemical analysis of A431 (Human epidermoid carcinoma cell line) whole cells labeling GTF2H1 in the nucleoplasm with ab204168 at 2 μg/ml.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GTF2H1 antibody (AB204168)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GTF2H1 antibody (AB204168)

Immunohistochemical analysis of human testis tissue labeling GTF2H1 (high expression) with ab204168 at a 1/50 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-GTF2H1 antibody (AB204168)
  • WB

Supplier Data

Western blot - Anti-GTF2H1 antibody (AB204168)

All lanes:

Western blot - Anti-GTF2H1 antibody (ab204168) at 1/250 dilution

Lane 1:

RT4 (Human urinary bladder cancer cell line) whole cell lysate

Lane 2:

U-251 MG (formally U-373 MG) (Human brain glioma cell line) whole cell lysate

Predicted band size: 62 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human GTF2H1 aa 50-200. The exact immunogen used to generate this antibody is proprietary information.

P32780

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GTF2H1 also known as Transcription Factor IIH Subunit 1 is a protein involved in transcription and DNA repair processes. The protein weighs approximately 71 kDa and consists of 667 amino acids. It functions as a part of the TFIIH complex. GTF2H1 is expressed in various tissues with higher levels observed in actively dividing cells suggesting its significant role in cellular proliferation activities. The protein’s complex interactions are indispensable for several DNA-dependent processes.
Biological function summary

GTF2H1 is a component of the TFIIH complex which has roles in both transcription initiation and nucleotide excision repair (NER). This complex is vital for the accurate transcription of DNA into RNA recognizing transcription factors and facilitating chromatin remodeling. Within NER GTF2H1 participates in unwinding DNA allowing the repair machinery to access damaged regions. Its activity ensures the maintenance of genomic stability which is essential for preventing mutations.

Pathways

GTF2H1 plays significant roles in the transcription initiation pathway and the DNA repair pathway. The protein interacts intricately with other members of the TFIIH complex like XPD and XPB which are helicases that modulate DNA unwinding during transcription and repair. These interactions are essential for coordinating the cellular response to DNA damage and regulating transcription by RNA polymerase II integrating the response with cell cycle control mechanisms.

Mutations or dysfunctions of GTF2H1 can lead to conditions like xeroderma pigmentosum and trichothiodystrophy. Both disorders are associated with defects in the nucleotide excision repair process. In these conditions proteins like XPD (also a part of TFIIH) are implicated which highlights the critical nature of GTF2H1 in maintaining DNA integrity. Such associations highlight the importance of thorough functional understanding of GTF2H1 in the development of therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription.
See full target information GTF2H1

Alternative Names

BTF2, GTF2H1, General transcription factor IIH subunit 1, Basic transcription factor 2 62 kDa subunit, General transcription factor IIH polypeptide 1, TFIIH basal transcription factor complex p62 subunit, BTF2 p62
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Publications (1)

Recent publications for all applications. Explore the full listand refine your search

Cancer cell international 19:237 PubMed31516394

2019

Cell-type specific concentration regulation of the basal transcription factor TFIIH in XPB mice model.

Applications

Unspecified application

Species

Unspecified reactive species

Lise-Marie Donnio,Catherine Miquel,Wim Vermeulen,Giuseppina Giglia-Mari,Pierre-Olivier Mari
View all publications
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Product promise

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