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AB232982

Anti-GTF2H1 antibody

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(2 Publications)

Rabbit Polyclonal GTF2H1 antibody. Suitable for WB, IHC-P and reacts with Pig, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GTF2H1 aa 50-350.

View Alternative Names

BTF2, GTF2H1, General transcription factor IIH subunit 1, Basic transcription factor 2 62 kDa subunit, General transcription factor IIH polypeptide 1, TFIIH basal transcription factor complex p62 subunit, BTF2 p62

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GTF2H1 antibody (AB232982)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GTF2H1 antibody (AB232982)

Formalin-fixed, paraffin-embedded human liver tissue stained for GTF2H1 with ab232982 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Western blot - Anti-GTF2H1 antibody (AB232982)
  • WB

Supplier Data

Western blot - Anti-GTF2H1 antibody (AB232982)

All lanes:

Western blot - Anti-GTF2H1 antibody (ab232982) at 5 µg/mL

All lanes:

Pig cerebrum tissue lysate

Secondary

All lanes:

HRP-Linked Goat anti-Rabbit IgG polyclonal at 0.2 µg/mL

Predicted band size: 62 kDa

false

Western blot - Anti-GTF2H1 antibody (AB232982)
  • WB

Supplier Data

Western blot - Anti-GTF2H1 antibody (AB232982)

All lanes:

Western blot - Anti-GTF2H1 antibody (ab232982) at 5 µg/mL

All lanes:

HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate

Secondary

All lanes:

HRP-Linked Goat anti-Rabbit IgG polyclonal at 0.2 µg/mL

Predicted band size: 62 kDa

false

Western blot - Anti-GTF2H1 antibody (AB232982)
  • WB

Supplier Data

Western blot - Anti-GTF2H1 antibody (AB232982)

All lanes:

Western blot - Anti-GTF2H1 antibody (ab232982) at 5 µg/mL

All lanes:

Pig liver tissue lysate

Secondary

All lanes:

HRP-Linked Goat anti-Rabbit IgG polyclonal at 0.2 µg/mL

Predicted band size: 62 kDa

false

Western blot - Anti-GTF2H1 antibody (AB232982)
  • WB

Supplier Data

Western blot - Anti-GTF2H1 antibody (AB232982)

All lanes:

Western blot - Anti-GTF2H1 antibody (ab232982) at 2 µg/mL

All lanes:

Recombinant human GTF2H1 protein

Predicted band size: 62 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Pig

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human GTF2H1 aa 50-350. The exact immunogen used to generate this antibody is proprietary information.

P32780

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Storage buffer
pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GTF2H1 also known as Transcription Factor IIH Subunit 1 is a protein involved in transcription and DNA repair processes. The protein weighs approximately 71 kDa and consists of 667 amino acids. It functions as a part of the TFIIH complex. GTF2H1 is expressed in various tissues with higher levels observed in actively dividing cells suggesting its significant role in cellular proliferation activities. The protein’s complex interactions are indispensable for several DNA-dependent processes.
Biological function summary

GTF2H1 is a component of the TFIIH complex which has roles in both transcription initiation and nucleotide excision repair (NER). This complex is vital for the accurate transcription of DNA into RNA recognizing transcription factors and facilitating chromatin remodeling. Within NER GTF2H1 participates in unwinding DNA allowing the repair machinery to access damaged regions. Its activity ensures the maintenance of genomic stability which is essential for preventing mutations.

Pathways

GTF2H1 plays significant roles in the transcription initiation pathway and the DNA repair pathway. The protein interacts intricately with other members of the TFIIH complex like XPD and XPB which are helicases that modulate DNA unwinding during transcription and repair. These interactions are essential for coordinating the cellular response to DNA damage and regulating transcription by RNA polymerase II integrating the response with cell cycle control mechanisms.

Mutations or dysfunctions of GTF2H1 can lead to conditions like xeroderma pigmentosum and trichothiodystrophy. Both disorders are associated with defects in the nucleotide excision repair process. In these conditions proteins like XPD (also a part of TFIIH) are implicated which highlights the critical nature of GTF2H1 in maintaining DNA integrity. Such associations highlight the importance of thorough functional understanding of GTF2H1 in the development of therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription.
See full target information GTF2H1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy. Nucleic acids 35:102380 PubMed39640012

2024

A lipid nanoparticle-based oligodendrocyte-specific mRNA therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Masanori Sawamura,Kiyoshi Tachikawa,Rie Hikawa,Hisako Akiyama,Seiji Kaji,Ken Yasuda,Angel I Leu,Hyojung Hong,Rajesh Mukthavaram,Pad Chivukula,Hodaka Yamakado,Yoshio Hirabayashi,Ryosuke Takahashi,Shu-Ichi Matsuzawa

eLife 11: PubMed35293859

2022

The TFIIH complex is required to establish and maintain mitotic chromosome structure.

Applications

Unspecified application

Species

Unspecified reactive species

Julian Haase,Richard Chen,Wesley M Parker,Mary Kate Bonner,Lisa M Jenkins,Alexander E Kelly
View all publications
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Product promise

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