Rabbit Polyclonal gtf3c3 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human General transcription factor 3C polypeptide 3.
View Alternative Names
General transcription factor 3C polypeptide 3, Transcription factor IIIC 102 kDa subunit, Transcription factor IIIC subunit gamma, TFIIIC 102 kDa subunit, TFIIIC102, TF3C-gamma, GTF3C3
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gtf3c3 antibody (AB74230)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling gtf3c3 with ab74230 at 1/1000 dilution.
- IP
Supplier Data
Immunoprecipitation - Anti-gtf3c3 antibody (AB74230)
gtf3c3 was immunoprecipitated from HEK293T whole cell lysate (1 mg per IP reaction, 20% loaded) with ab74230 at 3 μg per reaction. Western blot was performed on the immunoprecipitate using ab74230 at 1 μg/mL.
Lane 1 : ab74230 IP in HEK293T whole cell lysate.
Lane 2 : ab74230 IP in HEK293T whole cell lysate.
Lane 3 : Control IgG in HeLa whole cell lysate.
Detection : Chemiluminescence with an exposure time of 3 minutes
All lanes:
Immunoprecipitation - Anti-gtf3c3 antibody (ab74230)
Predicted band size: 101 kDa
false
- WB
Supplier Data
Western blot - Anti-gtf3c3 antibody (AB74230)
All lanes:
Western blot - Anti-gtf3c3 antibody (ab74230) at 0.1 µg/mL
Lane 1:
HeLa whole cell lysate at 50 µg
Lane 2:
Jurkat whole cell lysate at 50 µg
Predicted band size: 101 kDa
false
Exposure time: 3min
Reactivity data
Properties and storage information
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Appropriate short-term storage conditions
Appropriate long-term storage conditions
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Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
GTF3C3 functions as part of a larger multi-subunit complex called TFIIIC. This complex is necessary for the assembly of transcriptional machinery on DNA to initiate the transcription of small RNA genes by RNA polymerase III. The involvement of GTF3C3 in the TFIIIC complex indicates its critical role in maintaining normal cellular functions since these small RNAs are involved in protein synthesis and other essential cellular processes.
Pathways
The transcriptional activities of GTF3C3 align closely with the RNA polymerase III transcription pathway. This pathway is essential for the synthesis of small RNAs which play significant roles in the protein translation process. GTF3C3 interacts with other components of the TFIIIC complex contributing to the precise initiation of transcription. The pathway further involves coordination with proteins such as TFIIIB ensuring the proper functionality of RNA polymerase III in the transcription cycle.
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Target data
Publications (1)
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Molecular cell 77:475-487.e11 PubMed31759822
2019
Applications
Unspecified application
Species
Unspecified reactive species
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