Rabbit Recombinant Monoclonal RASH antibody. Suitable for WB, IP and reacts with Rat, Mouse, Human samples. Cited in 18 publications.
Images
![Western blot - Anti-GTPase HRAS antibody [Y132] (AB32417), expandable thumbnail](https://content.abcam.com/products/images/gtpase-hras-antibody-y132-ab32417--western-blot-img2351.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GTPase HRAS antibody [Y132] (AB32417)")
![Western blot - Anti-GTPase HRAS antibody [Y132] (AB32417), expandable thumbnail](https://content.abcam.com/products/images/gtpase-hras-antibody-y132-ab32417--western-blot-img4914.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GTPase HRAS antibody [Y132] (AB32417)")
![Western blot - Anti-GTPase HRAS antibody [Y132] (AB32417), expandable thumbnail](https://content.abcam.com/products/images/gtpase-hras-antibody-y132-ab32417--western-blot-img157316.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GTPase HRAS antibody [Y132] (AB32417)")
![Immunoprecipitation - Anti-GTPase HRAS antibody [Y132] (AB32417), expandable thumbnail](https://content.abcam.com/products/images/gtpase-hras-antibody-y132-ab32417--immunoprecipitation-img11409.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-GTPase HRAS antibody [Y132] (AB32417)")
Publications
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- BMC ophthalmology 22:4562022Blue light attenuates TGF-β2-induced epithelial-mesenchymal transition in human lens epithelial cells via autophagy impairment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDongyan Zhang,Hong Zhu,Xin Yu,Liyin Wang,Yingying Wen,Liyue Zhang,Jianping Tong,Ye ShenPubMed 36443719
- NPJ precision oncology 6:862022Co-targeting KRAS G12C and EGFR reduces both mutant and wild-type RAS-GTP.Applications:Unspecified applicationReactive species:Unspecified reactive speciesThomas McFall,Michael Trogdon,Anita C Guizar,John F Langenheim,Laura Sisk-Hackworth,Edward C StitesPubMed 36418474
- Cell reports 37:1100962021Identification of RAS mutant biomarkers for EGFR inhibitor sensitivity using a systems biochemical approach.Applications:Unspecified applicationReactive species:Unspecified reactive speciesThomas McFall,Edward C StitesPubMed 34910921
- Nature communications 11:53122020Siah2 integrates mitogenic and extracellular matrix signals linking neuronal progenitor ciliogenesis with germinal zone occupancy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTaren Ong et. al.PubMed 33082319
- Molecular and cellular biochemistry 476:175-1862020CircZNF609 promotes cell proliferation, migration, invasion, and glycolysis in nasopharyngeal carcinoma through regulating HRAS via miR-338-3p.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhonglu Liu,Feifei Liu,Fang Wang,Xin Yang,Wentao GuoPubMed 32970285
- Evidence-based complementary and alternative medic 2020:27609792020Ethanol Extract of the Infructescence of Sieb. et Zucc. Induces Methuosis of Human Nasopharyngeal Carcinoma Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinkun Liu et. al.PubMed 32419796
- Theranostics 9:6175-61902019DNA methylation-regulated QPCT promotes sunitinib resistance by increasing HRAS stability in renal cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTangliang Zhao et. al.PubMed 31534544
- Cell death & disease 7:e23112016PGA1-induced apoptosis involves specific activation of H-Ras and N-Ras in cellular endomembranes.
- The Journal of neuroscience : the official journal of the Society for Neuroscience 36:6431-442016Identification of PSD-95 Depalmitoylating Enzymes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNorihiko Yokoi,Yuko Fukata,Atsushi Sekiya,Tatsuro Murakami,Kenta Kobayashi,Masaki FukataPubMed 27307232
- Leukemia 30:1542-512016Loss of wild-type Kras promotes activation of all Ras isoforms in oncogenic Kras-induced leukemogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesG Kong et. al.PubMed 27055865
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| WB | IP | IHC | |
|---|---|---|---|
| Human | Tested | Expected | Not recommended |
| Mouse | Tested | Tested | Not recommended |
| Rat | Tested | Expected | Not recommended |
| Chicken | Predicted | Predicted | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/500 - 1/1000 | Notes - |
Species Mouse | Dilution info 1/500 - 1/1000 | Notes - |
Species Human | Dilution info 1/500 - 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/50 - 1/60 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken | Dilution info - | Notes - |
IHC
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human, Chicken | Dilution info - | Notes - |
Target data
Function
Involved in the activation of Ras protein signal transduction (PubMed:22821884). Ras proteins bind GDP/GTP and possess intrinsic GTPase activity (PubMed:12740440, PubMed:14500341, PubMed:9020151).
Alternative names
HRAS1, HRAS, GTPase HRas, H-Ras-1, Ha-Ras, Transforming protein p21, c-H-ras, p21ras
Recommended products
Rabbit Recombinant Monoclonal RASH antibody. Suitable for WB, IP and reacts with Rat, Mouse, Human samples. Cited in 18 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- Y132
- Purification technique
- Affinity purification Protein A
- Specificity
Reactivity with other RAS members has not been tested.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GTPase HRAS also known as H-RAS or HRAS1 is a small GTPase enzyme involved in intracellular signal transduction. It has a molecular mass of approximately 21 kDa. HRAS is widely expressed in various tissues and plays a role in regulating cell growth and differentiation. The HRAS protein functions as a molecular switch cycling between an active GTP-bound state and an inactive GDP-bound state. This activity is facilitated by the HRAS protein's intrinsic GTPase activity which hydrolyzes GTP to GDP.
- Biological function summary
GTPase HRAS participates in the regulation of cell proliferation differentiation and survival serving as an essential component of several signal transduction complexes. HRAS interacts with various signaling molecules and pathways to mediate mitogenic signals. Through these interactions the HRAS protein modulates cellular responses to extracellular stimuli influencing processes like growth and division playing an important role in maintaining normal cellular functions.
- Pathways
The involvement of GTPase HRAS in the MAPK/ERK and the PI3K/AKT pathways illustrates its importance in transmitting signals from cell surface receptors. These pathways control a variety of cellular functions including growth survival and metabolism. HRAS interacts with related proteins like KRAS and NRAS integral members of the RAS family. These interactions highlight its role in signaling cascades that contribute to the cellular response to growth factors.
- Associated diseases and disorders
Mutations and aberrant activation of GTPase HRAS link to oncogenic processes particularly in bladder and thyroid cancers. The mutated HRAS protein contributes to the unchecked cellular proliferation seen in tumorigenesis. Additionally HRAS mutations associate with developmental disorders such as Costello syndrome where aberrant signaling disrupts normal developmental processes. HRAS-related pathways often involve other RAS family members like KRAS underlining their collective impact in disease states.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-GTPase HRAS antibody [Y132] (ab32417), expandable thumbnail](https://content.abcam.com/products/images/gtpase-hras-antibody-y132-ab32417--western-blot-img2351.jpg "Western blot - Anti-GTPase HRAS antibody [Y132] (ab32417)")
Western blot - Anti-GTPase HRAS antibody [Y132] (ab32417)
Lanes 1 - 4: Merged signal (red and green). Green - ab32417 observed at 21 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.ab32417 was shown to recognize HRAS in wild-type HEK-293 cells as signal was lost at the expected MW in HRAS knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and HRAS knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. ab32417 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/500 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-GTPase HRAS antibody [Y132] (ab32417) at 1/500 dilution
Lane 1: Wild-type HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg
Lane 2: HRAS knockout HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg
Lane 2: Western blot - Human HRAS knockout HEK-293 cell line (Human HRAS knockout HEK-293 cell line ab260858)
Lane 3: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 4: MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 21 kDa
![Western blot - Anti-GTPase HRAS antibody [Y132] (ab32417), expandable thumbnail](https://content.abcam.com/products/images/gtpase-hras-antibody-y132-ab32417--western-blot-img4914.jpg "Western blot - Anti-GTPase HRAS antibody [Y132] (ab32417)")
Western blot - Anti-GTPase HRAS antibody [Y132] (ab32417)
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBSTAll lanes: Western blot - Anti-GTPase HRAS antibody [Y132] (ab32417) at 1/2500 dilution
Lane 1: mouse brain lysate at 10 µg
Lane 2: rat brain lysate at 10 µg
SecondaryAll lanes: HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa
![Western blot - Anti-GTPase HRAS antibody [Y132] (ab32417), expandable thumbnail](https://content.abcam.com/products/images/gtpase-hras-antibody-y132-ab32417--western-blot-img157316.jpg "Western blot - Anti-GTPase HRAS antibody [Y132] (ab32417)")
Western blot - Anti-GTPase HRAS antibody [Y132] (ab32417)
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBSTAll lanes: Western blot - Anti-GTPase HRAS antibody [Y132] (ab32417) at 1/1000 dilution
Lane 1: MCF7 cell lysate at 10 µg
Lane 2: HeLa cell lysate at 10 µg
SecondaryAll lanes: HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa
![Immunoprecipitation - Anti-GTPase HRAS antibody [Y132] (ab32417), expandable thumbnail](https://content.abcam.com/products/images/gtpase-hras-antibody-y132-ab32417--immunoprecipitation-img11409.jpg "Immunoprecipitation - Anti-GTPase HRAS antibody [Y132] (ab32417)")
Immunoprecipitation - Anti-GTPase HRAS antibody [Y132] (ab32417)
ab32417 (purified) at 1/60 immunoprecipitating GTPase in 10 μg mouse brain whole cell lysate (Lanes 1 and 2, observed at 21 kDa). Lane 3 - PBS. For western blotting, HRP Veriblot for IP Detection Reagent (VeriBlot for IP Detection Reagent (HRP) ab131366) was used for detection (1/10 000). Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST
All lanes: Immunoprecipitation - Anti-GTPase HRAS antibody [Y132] (ab32417)
Predicted band size: 21 kDa
![Western blot - Anti-GTPase HRAS antibody [Y132] (ab32417), expandable thumbnail](https://content.abcam.com/products/images/gtpase-hras-antibody-y132-ab32417--western-blot-img74684.gif "Western blot - Anti-GTPase HRAS antibody [Y132] (ab32417)")
Western blot - Anti-GTPase HRAS antibody [Y132] (ab32417)
All lanes: Western blot - Anti-GTPase HRAS antibody [Y132] (ab32417) at 1/500 dilution
Lane 1: MCF7 cell lysate
Lane 2: PC12 cell lysate
Predicted band size: 21 kDa
Observed band size: 21 kDa
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