AB200550

Anti-GTPBP1 antibody

Name: Anti-GTPBP1 antibody (ab200550) | Abcam
Brand: Abcam Limited
SKU: AB200550
Price: 535 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal GTPBP1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human GTPBP1 aa 550-650.

View Alternative Names

GTP-binding protein 1, G-protein 1, GP-1, GP1, GTPBP1

2 Images

Supplier Data

Immunoprecipitation - Anti-GTPBP1 antibody (AB200550)

Western blot analysis of immunoprecipitates of 293T whole cell lysate (0.5 or 1.0 mg per IP reaction) using ab200550 at 6 μg per reaction.

All lanes:

Immunoprecipitation - Anti-GTPBP1 antibody (ab200550) at 0.4 µg/mL

Lane 1:

Immunoprecipitates from 293T cells prepared using NETN lysis buffer (20% of immunoprecipitate loaded)

Lane 2:

Control IgG

Predicted band size: 72 kDa

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Supplier Data

Western blot - Anti-GTPBP1 antibody (AB200550)

All lanes:

Western blot - Anti-GTPBP1 antibody (ab200550) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Predicted band size: 72 kDa

false

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human GTPBP1 aa 550-650. The exact immunogen used to generate this antibody is proprietary information.

O00178

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cynomolgus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GTPBP1 also called GTP-binding protein 1 plays important roles in cellular processes. It is a member of the GTPase family which hydrolyzes GTP to GDP. GTPBP1 has a mass of about 73 kDa. It is found in various tissues throughout the body with higher expression in the brain liver and testis. GTPBP1 is known to be localized in the cytoplasm and is associated with the ribosome.

Biological function summary

GTPBP1 participates in ribosome biogenesis and RNA metabolism being involved in quality control of ribosomal RNA processing. It is part of a complex that aids the maturation and function of ribosomal units. This protein has roles in maintaining ribosomal integrity and ensuring proper translation which are essential for cellular function.

Pathways

GTPBP1 engages in important processes involved in protein synthesis and mitochondrial function. It interacts with the ribosomal RNA processing pathway and plays a part in mitochondrial biogenesis. Related proteins in these pathways include GTPBP2 another GTP-binding protein involved in similar ribosomal functions and proteins in the mitochondrial fission and fusion dynamics.

GTPBP1 has associations with neurodegenerative diseases and immune system disorders. Its dysfunction can contribute to neurodegenerative conditions such as Parkinson's disease. GTPBP1 may interact with proteins like alpha-synuclein which is implicated in Parkinson's pathology. Additionally disruptions in GTPBP1 expression can impact immune system balance potentially leading to autoimmune conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

GTPase that plays a role in the elongation phase of protein synthesis by forming ternary complexes with GTP and aminoacyl-transfer RNAs (aa-tRNAs), and delivering aa-tRNAs to the ribosomal A site in a GTP-dependent manner (PubMed : 30108131). Is also able to deliver deacylated tRNA to the A site (PubMed : 30108131). Additionally, it is involved in RNA quality control; after GTP hydrolysis, which is not immediately followed by rapid peptide bond formation, GTPBP1 likely retains aa-tRNA in the A site and promotes exosomal degradation of faulty mRNAs engaged in 80S elongation complexes (PubMed : 30108131). Plays a role in the regulation of circadian mRNA stability (By similarity).

See full target information GTPBP1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 23:85 PubMed39833825

2025

Modulation of DAPK1 expression by its alternative splice variant DAPK1-215 in cancer.

Applications

Unspecified application

Species

Unspecified reactive species

QingShui Wang,ShuYun Weng,WenTing Zhong,YouYu Lin,Yan Yu,YiMin Huang,LiLin Ge,XiuLi Zhang,FangQin Xue,Yehuda G Assaraf,Yao Lin

Protein science : a publication of the Protein Society 33:e4859 PubMed38145972

2023

Dynamic proximity interaction profiling suggests that YPEL2 is involved in cellular stress surveillance.

Applications

Unspecified application

Species

Unspecified reactive species

Gizem Turan,Çağla Ece Olgun,Hazal Ayten,Pelin Toker,Annageldi Ashyralyyev,Büşra Savaş,Ezgi Karaca,Mesut Muyan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GTPBP1 antibody

Immunoprecipitation - Anti-GTPBP1 antibody (AB200550)

Western blot - Anti-GTPBP1 antibody (AB200550)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise