AB184124

Anti-GTPBP4/NOG1 antibody

Name: Anti-GTPBP4/NOG1 antibody (ab184124) | Abcam
Brand: Abcam Limited
SKU: AB184124
Price: 545 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal GTPBP4/NOG1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GTPBP4 aa 250-600.

View Alternative Names

CRFG, NOG1, GTPBP4, GTP-binding protein 4, Chronic renal failure gene protein, GTP-binding protein NGB, Nucleolar GTP-binding protein 1

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-GTPBP4/NOG1 antibody (AB184124)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GTPBP4/NOG1 antibody (AB184124)

Immunocytochemical analysis of Hela cells fixed in 4% paraformaldehyde at room temperature for 15 mins labeling GTPBP4/NOG1 with ab184124 at 1/500 dilution (green) and counterstained with Hoechst 33342 (blue)

Supplier Data

Western blot - Anti-GTPBP4/NOG1 antibody (AB184124)

7.5% SDS PAGE

All lanes:

Western blot - Anti-GTPBP4/NOG1 antibody (ab184124) at 1/500 dilution

Lane 1:

Neuro2A whole cell lysate at 30 µg

Lane 2:

GL261 whole cell lysate whole cell lysate at 30 µg

Lane 3:

C8D30 whole cell lysate at 30 µg

Lane 4:

NIH-3T3 whole cell lysate at 30 µg

Lane 5:

BCL-1 whole cell lysate at 30 µg

Lane 6:

C2C12 whole cell lysate at 30 µg

Predicted band size: 74 kDa

false

Supplier Data

Western blot - Anti-GTPBP4/NOG1 antibody (AB184124)

7.5% SDS PAGE

All lanes:

Western blot - Anti-GTPBP4/NOG1 antibody (ab184124) at 1/500 dilution

Lane 1:

293T whole cell lysate

Lane 2:

A431 whole cell lysate

Lane 3:

HeLa whole cell lysate

Lane 4:

HepG2 whole cell lysate

Predicted band size: 74 kDa

false

Supplier Data

Western blot - Anti-GTPBP4/NOG1 antibody (AB184124)

All lanes:

Western blot - Anti-GTPBP4/NOG1 antibody (ab184124) at 1/500 dilution

Lane 1:

PC-12 whole cell lysate at 30 µg

Lane 2:

Rat2 whole cell lysate/ whole cell lysate at 30 µg

Predicted band size: 74 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GTPBP4/NOG1 antibody (AB184124)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GTPBP4/NOG1 antibody (AB184124)

Immunohistochemical staining of paraffin-embedded mouse ovary tissue showing nuclear staining labeling GTPBP4/NOG1 with ab184124 at 1/500 dilution

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human GTPBP4 aa 250-600. The exact immunogen used to generate this antibody is proprietary information.

Q9BZE4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GTPBP4 also known as NOG1 is a highly conserved protein involved in ribosome biogenesis. The protein has a molecular mass of approximately 72 kDa. GTPBP4 is a GTP-binding protein which belongs to the circularly permuted GTPase (cpGTPase) family. It typically locates in the nucleolus where it is involved in ribosomal RNA processing and assembly of the large ribosomal subunit. Expression of GTPBP4 is widespread in human tissues indicating its important role in cellular processes.

Biological function summary

The GTPBP4 protein contributes to the proper formation of the 60S ribosomal subunit through its role in ribosome assembly. It operates alongside other proteins as part of a complex. This role is important for maintaining normal levels of protein synthesis within cells. In particular GTPBP4 interacts with multiple ribosomal and nucleolar proteins to regulate ribosome assembly efficiently. Its activity ensures the structural maturation needed for the large subunit to join the small subunit during translation.

Pathways

GTPBP4 is a part of the ribosome biogenesis pathway. This pathway is important for the production of ribosomal RNA and assembly of ribosomal components. GTPBP4 works in conjunction with proteins like NPM1 and other nucleolar components to maintain the fidelity of ribosome production. It also plays a role in ensuring that the ribosomal subunits are properly assembled and functional for mRNA translation therefore linking the biogenesis of ribosomes with protein synthesis pathways.

Alterations in GTPBP4 expression or function can lead to various diseases such as cancer. GTPBP4 has connections to nucleolar stress responses which affect tumorigenesis. It interacts with proteins like MYC influencing cell proliferation and apoptosis. Dysregulation of GTPBP4 may disrupt ribosome biogenesis leading to imbalances in cell growth and division. It can also be involved in ribosomopathies disorders caused by defects in ribosome production impacting cellular protein synthesis and growth.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in the biogenesis of the 60S ribosomal subunit (PubMed : 32669547). Acts as a TP53 repressor, preventing TP53 stabilization and cell cycle arrest (PubMed : 20308539).

See full target information GTPBP4

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of oncology 2022:2164897 PubMed36405249

2022

GTPBP4: A New Therapeutic Target Gene Promotes Tumor Progression in Non-Small Cell Lung Cancer via EMT.

Applications

Unspecified application

Species

Unspecified reactive species

Junlu Wu,Guofei Chen,Weiwei Wang,Yang Yang,Yi Yuan,Anquan Shang,Wenqiang Quan,Lixin Wang

Life science alliance 1:e201800070 PubMed30456350

2018

Proteomics and neuropathology identify ribosomes as poly-GR/PR interactors driving toxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Hannelore Hartmann,Daniel Hornburg,Mareike Czuppa,Jakob Bader,Meike Michaelsen,Daniel Farny,Thomas Arzberger,Matthias Mann,Felix Meissner,Dieter Edbauer

Oncotarget 8:93984-93997 PubMed29212203

2017

Knockdown of GTPBP4 inhibits cell growth and survival in human hepatocellular carcinoma and its prognostic significance.

Applications

Species

Unspecified reactive species

Wen-Bin Liu,Wei-Dong Jia,Jin-Liang Ma,Ge-Liang Xu,Hang-Cheng Zhou,Yan Peng,Wei Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GTPBP4/NOG1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-GTPBP4/NOG1 antibody (AB184124)

Western blot - Anti-GTPBP4/NOG1 antibody (AB184124)

Western blot - Anti-GTPBP4/NOG1 antibody (AB184124)

Western blot - Anti-GTPBP4/NOG1 antibody (AB184124)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GTPBP4/NOG1 antibody (AB184124)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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