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AB247546

Anti-GTPBP4/NOG1 antibody [EPR3500] - BSA and Azide free

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Rabbit Recombinant Monoclonal GTPBP4/NOG1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.

View Alternative Names

CRFG, NOG1, GTPBP4, GTP-binding protein 4, Chronic renal failure gene protein, GTP-binding protein NGB, Nucleolar GTP-binding protein 1

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GTPBP4/NOG1 antibody [EPR3500] - BSA and Azide free (AB247546)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GTPBP4/NOG1 antibody [EPR3500] - BSA and Azide free (AB247546)

This data was developed using ab92342, the same antibody clone in a different buffer formulation.

ab92342 at 1/250 dilution staining GTPBP4/NOG1 in Human kidney by Immunohistochemistry, Paraffin-embedded tissue. The use of an HRP/AP polymerized antibody is recommended for a secondary antibody.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-GTPBP4/NOG1 antibody [EPR3500] - BSA and Azide free (AB247546)
  • WB

Unknown

Western blot - Anti-GTPBP4/NOG1 antibody [EPR3500] - BSA and Azide free (AB247546)

This data was developed using ab92342, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-GTPBP4/NOG1 antibody [EPR3500] (<a href='/en-us/products/primary-antibodies/gtpbp4-nog1-antibody-epr3500-ab92342'>ab92342</a>) at 1/2000 dilution

Lane 1:

Human fetal kidney tissue lysate at 10 µg

Lane 2:

Human fetal brain tissue lysate at 10 µg

Lane 3:

Human placenta tissue lysate at 10 µg

Lane 4:

Human testis tissue lysate at 10 µg

Secondary

All lanes:

goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 74 kDa

Observed band size: 80 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3500

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab247546 is the carrier-free version of ab92342.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GTPBP4 also known as NOG1 is a highly conserved protein involved in ribosome biogenesis. The protein has a molecular mass of approximately 72 kDa. GTPBP4 is a GTP-binding protein which belongs to the circularly permuted GTPase (cpGTPase) family. It typically locates in the nucleolus where it is involved in ribosomal RNA processing and assembly of the large ribosomal subunit. Expression of GTPBP4 is widespread in human tissues indicating its important role in cellular processes.
Biological function summary

The GTPBP4 protein contributes to the proper formation of the 60S ribosomal subunit through its role in ribosome assembly. It operates alongside other proteins as part of a complex. This role is important for maintaining normal levels of protein synthesis within cells. In particular GTPBP4 interacts with multiple ribosomal and nucleolar proteins to regulate ribosome assembly efficiently. Its activity ensures the structural maturation needed for the large subunit to join the small subunit during translation.

Pathways

GTPBP4 is a part of the ribosome biogenesis pathway. This pathway is important for the production of ribosomal RNA and assembly of ribosomal components. GTPBP4 works in conjunction with proteins like NPM1 and other nucleolar components to maintain the fidelity of ribosome production. It also plays a role in ensuring that the ribosomal subunits are properly assembled and functional for mRNA translation therefore linking the biogenesis of ribosomes with protein synthesis pathways.

Alterations in GTPBP4 expression or function can lead to various diseases such as cancer. GTPBP4 has connections to nucleolar stress responses which affect tumorigenesis. It interacts with proteins like MYC influencing cell proliferation and apoptosis. Dysregulation of GTPBP4 may disrupt ribosome biogenesis leading to imbalances in cell growth and division. It can also be involved in ribosomopathies disorders caused by defects in ribosome production impacting cellular protein synthesis and growth.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in the biogenesis of the 60S ribosomal subunit (PubMed : 32669547). Acts as a TP53 repressor, preventing TP53 stabilization and cell cycle arrest (PubMed : 20308539).
See full target information GTPBP4
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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