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AB223727

Anti-Guanylin antibody

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(1 Publication)

Rabbit Polyclonal Guanylin antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human GUCA2A aa 1 to C-terminus.

View Alternative Names

GUCA2, GUCA2A, Guanylin, Guanylate cyclase activator 2A, Guanylate cyclase-activating protein 1, Guanylate cyclase-activating protein I, GCAP-I

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Guanylin antibody (AB223727)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Guanylin antibody (AB223727)

Paraffin embedded human small intestine tissue stained for Guanylin with ab223727 (1/1000 dilution) in immunohistochemical analysis.

Western blot - Anti-Guanylin antibody (AB223727)
  • WB

Supplier Data

Western blot - Anti-Guanylin antibody (AB223727)

All lanes:

Western blot - Anti-Guanylin antibody (ab223727) at 1/100 dilution

Lane 1:

Control (vector only transfected HEK293T lysate)

Lane 2:

Guanylin over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK-293T cells

Predicted band size: 12 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human GUCA2A aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q02747

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Guanylin sometimes referred to as guanylate cyclase activator is a peptide hormone with a molecular mass of approximately 3.8 kDa. It is primarily expressed in the intestinal epithelium but can also be found in other tissues such as the kidney and reproductive system. Guanylin operates mechanically by binding to and activating guanylate cyclase C (GC-C) on the cell surface which leads to increased levels of cyclic guanosine monophosphate (cGMP) within the cells influencing fluid and electrolyte transport.
Biological function summary

Guanylin influences several physiological processes important for maintaining homeostasis in the gut. This peptide forms part of the guanylin family of peptides which also includes uroguanylin. It functions by modulating ion and water transport in the intestines playing a role in the regulation of fluid balance and intestinal pH. Through its action guanylin promotes the secretion of chloride and bicarbonate while inhibiting the absorption of sodium therefore facilitating bowel movement and fluid homeostasis.

Pathways

Guanylin is an important component in signaling through the cGMP pathway important for maintaining intestinal fluidity and pH balance. It plays a role in activating the guanylate cyclase C pathway leading to downstream signaling events involving protein kinase G (PKG) and cystic fibrosis transmembrane conductance regulator (CFTR) channels regulating electrolyte transport. This pathway is important for maintaining gastrointestinal motility and the barrier function of the intestine.

Reduced guanylin activity has been associated with conditions like colorectal cancer and cystic fibrosis. In colorectal cancer the loss of guanylin expression leads to disrupted cGMP signaling contributing to tumorigenesis. In cystic fibrosis although guanylin itself is not directly defective it interacts with CFTR which is dysfunctional in this disorder influencing fluid transport and contributing to characteristic symptoms. Understanding guanylin's role provides insights into therapeutic targets for these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Endogenous activator of intestinal guanylate cyclase. It stimulates this enzyme through the same receptor binding region as the heat-stable enterotoxins.
See full target information GUCA2A
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

World journal of gastrointestinal oncology 16:787-797 PubMed38577466

2024

High patatin like phospholipase domain containing 8 expression as a biomarker for poor prognosis of colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Peng-Yang Zhou,De-Xiang Zhu,Yi-Jiao Chen,Qing-Yang Feng,Yi-Hao Mao,Ao-Bo Zhuang,Jian-Min Xu
View all publications
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