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AB249697

Anti-Guanylyl Cyclase alpha 1/GUCY1A3 antibody [EPR12270] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal Guanylyl Cyclase alpha 1/GUCY1A3 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

GUC1A3, GUCSA3, GUCY1A3, GUCY1A1, Guanylate cyclase soluble subunit alpha-1, GCS-alpha-1, Guanylate cyclase soluble subunit alpha-3, Soluble guanylate cyclase large subunit, GCS-alpha-3

1 Images
Western blot - Anti-Guanylyl Cyclase alpha 1/GUCY1A3 antibody [EPR12270] - BSA and Azide free (AB249697)
  • WB

Supplier Data

Western blot - Anti-Guanylyl Cyclase alpha 1/GUCY1A3 antibody [EPR12270] - BSA and Azide free (AB249697)

This data was developed using ab172480, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Guanylyl Cyclase alpha 1/GUCY1A3 antibody [EPR12270] (<a href='/en-us/products/primary-antibodies/guanylyl-cyclase-alpha-1-gucy1a3-antibody-epr12270-ab172480'>ab172480</a>) at 1/1000 dilution

Lane 1:

Human fetal kidney tissue lysate at 10 µg

Lane 2:

Human fetal lung tissue lysate at 10 µg

Predicted band size: 77 kDa

false

  • Unconjugated

    Anti-Guanylyl Cyclase alpha 1/GUCY1A3 antibody [EPR12270]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12270

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

ab249697 is the carrier-free version of ab172480.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Guanylyl Cyclase alpha 1 also known as GUCY1A3 is a subunit of the soluble guanylyl cyclase enzyme often referred to simply as guanylyl cyclase. This enzyme plays an important mechanical role by synthesizing cyclic guanosine monophosphate (cGMP) from guanosine triphosphate (GTP). The GUCY1A3 subunit exists primarily as part of a heterodimer typically pairing with the beta subunit GUCY1B3 to form the active enzyme complex. The protein has an approximate mass of 70 kDa and is widely expressed in various tissues with abundant expression in vascular smooth muscle cells platelets and neurons.
Biological function summary

The guanylyl cyclase enzyme containing GUCY1A3 acts as a signal transducer in nitric oxide (NO) signaling pathways. The enzyme activity governed by GUCY1A3 produces cGMP in response to NO which then functions as a secondary messenger. This secondary messenger plays an important role in vasodilation inhibiting platelet aggregation and neurotransmission. The guanylyl cyclase-GUCY1A3 complex significantly influences vascular tone and hence blood pressure regulation.

Pathways

GUCY1A3 integrates firmly into critical biological routes like the nitric oxide-cGMP pathway and the cGMP-PKG signaling pathway. In the nitric oxide-cGMP pathway GUCY1A3-related enzymatic activity leads to smooth muscle relaxation a pivotal process in cardiovascular physiology. Through its involvement in the cGMP-PKG pathway GUCY1A3 influences signal transductions that affect myocardial contractility and neuronal signaling. Interactions with other proteins such as PKG (protein kinase G) serve as downstream signaling processes initiated by cGMP.

Alterations in GUCY1A3 function associate with cardiovascular diseases like hypertension and pulmonary arterial hypertension. Disruption in GUCY1A3 activity affects normal NO-cGMP signaling leading to inappropriate blood pressure control and vascular anomalies. Moreover genetic mutations or dysregulation of GUCY1A3 have links to thrombosis primarily due to its role in modulating platelet activity through the NO-cGMP pathway. The protein connexin to disorders typically involves its interactions with soluble guanylyl cyclase subunit beta (GUCY1B3) as imbalances in their complex formation often result in impaired vascular homeostasis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information GUCY1A1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oxidative medicine and cellular longevity 2021:8379962 PubMed34630853

2021

circ_0023461 Silencing Protects Cardiomyocytes from Hypoxia-Induced Dysfunction through Targeting miR-370-3p/PDE4D Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Ren,Buying Li,Liqing Jiang,Zhiheng Liu,Fan Wu,Yi Zhang,Jincheng Liu,Weixun Duan
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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