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AB42108

Anti-GUCY1A2 antibody

4

(1 Review)

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(10 Publications)

Rabbit Polyclonal GUCY1A2 antibody. Suitable for WB, IHC-P and reacts with Recombinant full length protein - Rat, Mouse samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human GUCY1A2 aa 650 to C-terminus.

View Alternative Names

GUC1A2, GUCSA2, GUCY1A2, Guanylate cyclase soluble subunit alpha-2, GCS-alpha-2

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1A2 antibody (AB42108)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1A2 antibody (AB42108)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse large intestine staining GUCY1A2 with ab42108 at a 1/100 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnificiation.

Western blot - Anti-GUCY1A2 antibody (AB42108)
  • WB

Unknown

Western blot - Anti-GUCY1A2 antibody (AB42108)

All lanes:

Western blot - Anti-GUCY1A2 antibody (ab42108) at 1/750 dilution

All lanes:

Soluble Guanylyl Cyclase alpha 2 Antibody Positive Control (Affinity Purified Recombinant full length protein)

Predicted band size: 82 kDa

Observed band size: 98 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human GUCY1A2 aa 650 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P33402

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/750", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/150", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant full length protein - Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/750", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The GUCY1A2 protein also known as guanylate cyclase soluble subunit alpha-2 is an enzyme important in the conversion of GTP to cyclic GMP. This protein has a molecular mass of about 77 kDa. GUCY1A2 is expressed in various tissues including the brain heart and lung supporting diverse physiological functions. The enzyme functions as part of a larger heterodimeric complex binding with another subunit to become active.
Biological function summary

This enzyme plays an essential role in the nitric oxide (NO) signaling pathway. GUCY1A2 as a subunit of soluble guanylate cyclase (sGC) complex binds with nitric oxide to produce cyclic GMP a secondary messenger that influences many physiological processes. These processes include vascular smooth muscle relaxation and neurotransmission both of which depend on the synthesis of cyclic GMP. The balance and regulation of this pathway are critical to maintaining normal cell functions.

Pathways

GUCY1A2 engages significantly in the NO-cGMP signaling pathway and the vascular smooth muscle contraction pathway. Through its role in the NO-cGMP signaling pathway GUCY1A2 collaborates with proteins like sGC subunit beta-1 (GUCY1B1) to facilitate cyclic GMP production. This pathway plays an important function in regulating vascular tone and neuronal signaling both of which are important for homeostasis.

Dysregulation of GUCY1A2 function associates with conditions like pulmonary hypertension and cardiovascular diseases. In pulmonary hypertension the improper production or response to NO impairs normal cGMP synthesis contributing to increased vascular resistance. The protein's interaction with GUCY1B1 becomes relevant here as alterations in GUCY1A2 impact the heterodimeric guanylate cyclase's activity leading to heightened disease risk. Understanding this relationship is key to developing targeted therapeutics for related disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Has guanylyl cyclase on binding to the beta-1 subunit.. Isoform 2 acts as a negative regulator of guanylyl cyclase activity as it forms non-functional heterodimers with the beta subunits.
See full target information GUCY1A2
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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

World journal of surgical oncology 21:61 PubMed36823639

2023

Identification of critical prognosis signature associated with lymph node metastasis of stomach adenocarcinomas.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohui Wang,Wei Zhang,Yulin Guo,Yifei Zhang,Xiaofeng Bai,Yibin Xie

Evidence-based complementary and alternative medicine : eCAM 2021:5579129 PubMed34055010

2021

Network Pharmacology Prediction and Pharmacological Verification Mechanism of Yeju Jiangya Decoction on Hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Wang,Mao He,Yuzhong Du,Suhong Chen,Guiyuan Lv

The European journal of neuroscience 51:1881-1899 PubMed32115781

2020

A role for nitric oxide in serotonin neurons of the midbrain raphe nuclei.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah E Gartside,Abdurrahman Ercan Yurttaser,Amy L Burns,Nebojša Jovanović,Katie J Smith,Nana Shika Amegashiti,Bas M J Olthof

The Journal of neuroscience : the official journal 39:876-887 PubMed30530507

2018

Puncta of Neuronal Nitric Oxide Synthase (nNOS) Mediate NMDA Receptor Signaling in the Auditory Midbrain.

Applications

Unspecified application

Species

Unspecified reactive species

Bas M J Olthof,Sarah E Gartside,Adrian Rees

Frontiers in cellular neuroscience 12:369 PubMed30405353

2018

Modulation of Hyperpolarization-Activated Inward Current and Thalamic Activity Modes by Different Cyclic Nucleotides.

Applications

Unspecified application

Species

Unspecified reactive species

Maia Datunashvili,Rahul Chaudhary,Mehrnoush Zobeiri,Annika Lüttjohann,Evanthia Mergia,Arnd Baumann,Sabine Balfanz,Björn Budde,Gilles van Luijtelaar,Hans-Christian Pape,Doris Koesling,Thomas Budde

Nitric oxide : biology and chemistry 78:1-10 PubMed29698689

2018

Nitric oxide induces epidermal stem cell de-adhesion by targeting integrin β1 and Talin via the cGMP signalling pathway.

Applications

WB

Species

Unspecified reactive species

Rixing Zhan,Fan Wang,Ying Wu,Ying Wang,Wei Qian,Menglong Liu,Tengfei Liu,Weifeng He,Hui Ren,Gaoxing Luo

Nature communications 6:8482 PubMed26442659

2015

Cardiovascular and pharmacological implications of haem-deficient NO-unresponsive soluble guanylate cyclase knock-in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Robrecht Thoonen,Anje Cauwels,Kelly Decaluwe,Sandra Geschka,Robert E Tainsh,Joris Delanghe,Tino Hochepied,Lode De Cauwer,Elke Rogge,Sofie Voet,Patrick Sips,Richard H Karas,Kenneth D Bloch,Marnik Vuylsteke,Johannes-Peter Stasch,Johan Van de Voorde,Emmanuel S Buys,Peter Brouckaert

American journal of physiology. Heart and circulat 297:H654-63 PubMed19502556

2009

sGC(alpha)1(beta)1 attenuates cardiac dysfunction and mortality in murine inflammatory shock models.

Applications

WB

Species

Mouse

Emmanuel S Buys,Anje Cauwels,Michael J Raher,Jonathan J Passeri,Ion Hobai,Sharon M Cawley,Kristen M Rauwerdink,Helene Thibault,Patrick Y Sips,Robrecht Thoonen,Marielle Scherrer-Crosbie,Fumito Ichinose,Peter Brouckaert,Kenneth D Bloch

Naunyn-Schmiedeberg's archives of pharmacology 378:493-502 PubMed18563392

2008

Role of the soluble guanylyl cyclase alpha1/alpha2 subunits in the relaxant effect of CO and CORM-2 in murine gastric fundus.

Applications

WB

Species

Mouse

Ole De Backer,Ellen Elinck,Patrick Sips,Emmanuel Buys,Peter Brouckaert,Romain A Lefebvre

American journal of physiology. Lung cellular and molecular physiology 293:L1261-70 PubMed17827249

2007

Maturational changes in the regulation of pulmonary vascular tone by nitric oxide in neonatal rats.

Applications

Unspecified application

Species

Unspecified reactive species

Louis G Chicoine,Michael L Paffett,Mark R Girton,Matthew J Metropoulus,Mandar S Joshi,John Anthony Bauer,Leif D Nelin,Thomas C Resta,Benjimen R Walker
View all publications
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