AB24824

Anti-GUCY1B3 antibody

(1 Review)

(8 Publications)

Rabbit Polyclonal GUCY1B3 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 8 publications.

View Alternative Names

GUC1B3, GUCSB3, GUCY1B3, GUCY1B1, Guanylate cyclase soluble subunit beta-1, GCS-beta-1, Guanylate cyclase soluble subunit beta-3, Soluble guanylate cyclase small subunit, GCS-beta-3

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-GUCY1B3 antibody (AB24824)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-GUCY1B3 antibody (AB24824)

ICC/IF image of ab24824 stained SKNSH cells. The cells were Both and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24824, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody (AB24824)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody (AB24824)

Human cerebellum stained with Anti GUCY1B3 ab24824 at 1/100 dilution for 30 min at RT.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GUCY1B3 also known as guanylate cyclase soluble subunit beta-1 serves as a subunit of the soluble guanylate cyclase (sGC) complex. This protein acts in converting GTP to cGMP which plays an important role in intracellular signaling. GUCY1B3 has a molecular mass of approximately 70 kDa. The expression of GUCY1B3 occurs in a variety of tissues with higher levels observed in smooth muscle cells and neurons.

Biological function summary

The GUCY1B3 protein functions as part of the soluble guanylate cyclase complex which directly responds to nitric oxide (NO) signaling. This NO binding leads to the production of cyclic GMP a secondary messenger involved in numerous biological responses. These responses include vasodilation and neurotransmission. GUCY1B3's activity regulates essential cellular processes through these mechanisms.

Pathways

The sGC complex involving GUCY1B3 participates in the nitric oxide signaling pathway which is significant for cardiovascular function. Additionally GUCY1B3 works alongside GUCY1A3 another subunit of the sGC complex to facilitate this signaling cascade. The resulting cGMP acts in downstream pathways like the cGMP-dependent protein kinase G (PKG) pathway which controls vascular tone and cardiac function.

GUCY1B3 plays a role in complications such as hypertension and pulmonary hypertension. Disruption in NO signaling involving GUCY1B3 can result in impaired vasodilation contributing to these conditions. Moreover alterations in the function of GUCY1A3 the partner in the sGC complex can affect the same pathways further implicating GUCY1B3 in the pathology of cardiovascular disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Mediates responses to nitric oxide (NO) by catalyzing the biosynthesis of the signaling molecule cGMP.

See full target information GUCY1B1

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

iScience 24:102976 PubMed34485858

2021

Podoplanin drives dedifferentiation and amoeboid invasion of melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Charlotte M de Winde,Samantha L George,Eva Crosas-Molist,Yukti Hari-Gupta,Abbey B Arp,Agnesska C Benjamin,Lindsey J Millward,Spyridon Makris,Alexander Carver,Valerio Imperatore,Víctor G Martínez,Victoria Sanz-Moreno,Sophie E Acton

Journal of cell science 134: PubMed34184727

2021

Fibroblastic reticular cell response to dendritic cells requires coordinated activity of podoplanin, CD44 and CD9.

Applications

Unspecified application

Species

Unspecified reactive species

Charlotte M de Winde,Spyridon Makris,Lindsey J Millward,Jesús A Cantoral-Rebordinos,Agnesska C Benjamin,Víctor G Martínez,Sophie E Acton

Frontiers in physiology 11:700 PubMed32655417

2020

Is Innervation of the Neuromuscular Junction at the Diaphragm Modulated by sGC/cGMP Signaling?

Applications

Unspecified application

Species

Unspecified reactive species

Nadežda Lukáčová,L'udmila Hricová,Alexandra Kisucká,Štefánia Papcunová,Katarína Bimbová,Mária Bačová,Jaroslav Pavel,Martin Marsala,Ivo Vanický,Zuzana Dzurjašková,Stanislav Matéffy,Viktória Lukáčová,Andrea Stropkovská,Ján Gálik

Journal of molecular and cellular cardiology 137:119-131 PubMed31669609

2019

Diastolic dysfunction is initiated by cardiomyocyte impairment ahead of endothelial dysfunction due to increased oxidative stress and inflammation in an experimental prediabetes model.

Applications

Unspecified application

Species

Unspecified reactive species

Mark T Waddingham,Takashi Sonobe,Hirotsugu Tsuchimochi,Amanda J Edgley,Vijayakumar Sukumaran,Yi Ching Chen,Sarabjit S Hansra,Daryl O Schwenke,Keiji Umetani,Kohki Aoyama,Naoto Yagi,Darren J Kelly,Shahrooz Gaderi,Melissa Herwig,Detmar Kolijn,Andreas Mügge,Walter J Paulus,Takeshi Ogo,Mikiyasu Shirai,Nazha Hamdani,James T Pearson

Canadian journal of physiology and pharmacology 95:356-364 PubMed28103056

2017

Age-related changes to vascular protease-activated receptor 2 in metabolic syndrome: a relationship between oxidative stress, receptor expression, and endothelium-dependent vasodilation.

Applications

Unspecified application

Species

Unspecified reactive species

Kana Maruyama,Satomi Kagota,John J McGuire,Hirokazu Wakuda,Noriko Yoshikawa,Kazuki Nakamura,Kazumasa Shinozuka

Nitric oxide : biology and chemistry 35:165-74 PubMed24149190

2013

Nitric oxide is less effective at inhibiting neointimal hyperplasia in spontaneously hypertensive rats.

Applications

IHC-P

Species

Rat

Nick D Tsihlis,Ashley K Vavra,Janet Martinez,Vanessa R Lee,Melina R Kibbe

Circulation 126:830-9 PubMed22806632

2012

Low myocardial protein kinase G activity in heart failure with preserved ejection fraction.

Applications

IHC-P

Species

Human

Loek van Heerebeek,Nazha Hamdani,Inês Falcão-Pires,Adelino F Leite-Moreira,Mark P V Begieneman,Jean G F Bronzwaer,Jolanda van der Velden,Ger J M Stienen,Gerrit J Laarman,Aernout Somsen,Freek W A Verheugt,Hans W M Niessen,Walter J Paulus

The European journal of neuroscience 28:813-21 PubMed18671741

2008

Oxytocin induces penile erection when injected into the ventral tegmental area of male rats: role of nitric oxide and cyclic GMP.

Applications

IHC-FoFr

Species

Rat

Salvatora Succu,Fabrizio Sanna,Cristina Cocco,Tiziana Melis,Antonio Boi,Gian-Luca Ferri,Antonio Argiolas,Maria Rosaria Melis

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GUCY1B3 antibody

Immunocytochemistry/ Immunofluorescence - Anti-GUCY1B3 antibody (AB24824)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody (AB24824)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Product promise