Name: Anti-GUCY1B3 antibody [EPR8822]
SKU: ab154841
Rating: 4 (2 reviews)

Rabbit Recombinant Monoclonal GUCY1B3 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 12 publications.

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody [EPR8822] (AB154841), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	WB
Human	Tested	Tested
Mouse	Expected	Tested
Rat	Expected	Tested

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/400	Notes The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. For unpurified use at 1/100 -1/250. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000 - 1/10000	Notes -
Species Rat	Dilution info 1/1000 - 1/10000	Notes -
Species Human	Dilution info 1/1000 - 1/10000	Notes -

Associated Products

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Target data

See full target information GUCY1B1

Function

Mediates responses to nitric oxide (NO) by catalyzing the biosynthesis of the signaling molecule cGMP.

Alternative names

GUC1B3, GUCSB3, GUCY1B3, GUCY1B1, Guanylate cyclase soluble subunit beta-1, GCS-beta-1, Guanylate cyclase soluble subunit beta-3, Soluble guanylate cyclase small subunit, GCS-beta-3

Recommended products

Rabbit Recombinant Monoclonal GUCY1B3 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 12 publications.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR8822
Purification technique: Affinity purification Protein A
Specificity: The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

GUCY1B3 also known as guanylate cyclase soluble subunit beta-1 serves as a subunit of the soluble guanylate cyclase (sGC) complex. This protein acts in converting GTP to cGMP which plays an important role in intracellular signaling. GUCY1B3 has a molecular mass of approximately 70 kDa. The expression of GUCY1B3 occurs in a variety of tissues with higher levels observed in smooth muscle cells and neurons.

Biological function summary

The GUCY1B3 protein functions as part of the soluble guanylate cyclase complex which directly responds to nitric oxide (NO) signaling. This NO binding leads to the production of cyclic GMP a secondary messenger involved in numerous biological responses. These responses include vasodilation and neurotransmission. GUCY1B3's activity regulates essential cellular processes through these mechanisms.

Pathways

The sGC complex involving GUCY1B3 participates in the nitric oxide signaling pathway which is significant for cardiovascular function. Additionally GUCY1B3 works alongside GUCY1A3 another subunit of the sGC complex to facilitate this signaling cascade. The resulting cGMP acts in downstream pathways like the cGMP-dependent protein kinase G (PKG) pathway which controls vascular tone and cardiac function.

Associated diseases and disorders

GUCY1B3 plays a role in complications such as hypertension and pulmonary hypertension. Disruption in NO signaling involving GUCY1B3 can result in impaired vasodilation contributing to these conditions. Moreover alterations in the function of GUCY1A3 the partner in the sGC complex can affect the same pathways further implicating GUCY1B3 in the pathology of cardiovascular disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody [EPR8822] (ab154841)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue sections labeling GUCY1B3 with Purified ab154841 at 1:400 dilution (2.54 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
Western blot - Anti-GUCY1B3 antibody [EPR8822] (ab154841)
Blocking and diluting buffer : 5% NFDM/TBST
All lanes: Western blot - Anti-GUCY1B3 antibody [EPR8822] (ab154841) at 1/5000 dilution
Lane 1: Human fetal lung lysates at 20 µg
Lane 2: Mouse brain lysates at 20 µg
Lane 3: Rat brain lysates at 20 µg
Lane 4: Rat spleen lysates at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 24 kDa, 38 kDa, 52 kDa, 60 kDa, 68 kDa, 70 kDa, 71 kDa
Observed band size: 38 kDa, 80 kDa
Western blot - Anti-GUCY1B3 antibody [EPR8822] (ab154841)
All lanes: Western blot - Anti-GUCY1B3 antibody [EPR8822] (ab154841) at 1/1000 dilution
Lane 1: Human placenta lysate at 10 µg
Lane 2: Human fetal brain lysate at 10 µg
Lane 3: Human fetal lung lysate at 10 µg
Predicted band size: 71 kDa
Western blot - Anti-GUCY1B3 antibody [EPR8822] (ab154841)
Blocking and diluting buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-GUCY1B3 antibody [EPR8822] (ab154841) at 1/2000 dilution
Lane 1: Mouse brain tissue lysate at 20 µg
Lane 2: Mouse heart tissue lysate at 20 µg
Lane 3: Mouse kidney tissue lysate at 20 µg
Lane 4: Mouse spleen tissue lysate at 20 µg
Lane 5: Rat brain tissue lysate at 20 µg
Lane 6: Rat heart tissue lysate at 20 µg
Lane 7: Rat kidney tissue lysate at 20 µg
Lane 8: Rat spleen tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 71 kDa
Observed band size: 65 kDa
Exposure time: 3min
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody [EPR8822] (ab154841)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue, labeling GUCY1B3 using unpurified ab154841 at a 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody [EPR8822] (ab154841)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human thyroid gland carcinoma tissue, labeling GUCY1B3 using unpurified ab154841 at a 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody [EPR8822] (ab154841)
Immunohistochemical analysis of paraffin embedded Human normal kidney tissue using unpurified ab154841 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody [EPR8822] (ab154841)
Immunohistochemical analysis of paraffin embedded Human heart tissue using unpurified ab154841 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody [EPR8822] (ab154841)
Immunohistochemical analysis of paraffin embedded Human endometrial carcinoma tissue using unpurified ab154841 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.