AB154841

Anti-GUCY1B3 antibody [EPR8822]

RabMAb
Recombinant
What is this?

4

(2 Reviews)

|

(14 Publications)

Rabbit Recombinant Monoclonal GUCY1B3 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 14 publications.

View Alternative Names

GUC1B3, GUCSB3, GUCY1B3, GUCY1B1, Guanylate cyclase soluble subunit beta-1, GCS-beta-1, Guanylate cyclase soluble subunit beta-3, Soluble guanylate cyclase small subunit, GCS-beta-3

9 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody [EPR8822] (AB154841)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody [EPR8822] (AB154841)

Immunohistochemical analysis of paraffin embedded Human heart tissue using unpurified ab154841 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody [EPR8822] (AB154841)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody [EPR8822] (AB154841)

Immunohistochemical analysis of paraffin embedded Human endometrial carcinoma tissue using unpurified ab154841 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody [EPR8822] (AB154841)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody [EPR8822] (AB154841)

Immunohistochemical analysis of paraffin embedded Human normal kidney tissue using unpurified ab154841 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody [EPR8822] (AB154841)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody [EPR8822] (AB154841)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human thyroid gland carcinoma tissue, labeling GUCY1B3 using unpurified ab154841 at a 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody [EPR8822] (AB154841)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody [EPR8822] (AB154841)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue, labeling GUCY1B3 using unpurified ab154841 at a 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody [EPR8822] (AB154841)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody [EPR8822] (AB154841)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue sections labeling GUCY1B3 with Purified ab154841 at 1 : 400 dilution (2.54 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1 : 0 dilution. PBS instead of the primary antibody was used as the negative control.

Western blot - Anti-GUCY1B3 antibody [EPR8822] (AB154841)

WB

Unknown

Western blot - Anti-GUCY1B3 antibody [EPR8822] (AB154841)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-GUCY1B3 antibody [EPR8822] (ab154841) at 1/5000 dilution

Lane 1:

Human fetal lung lysates at 20 µg

Lane 2:

Mouse brain lysates at 20 µg

Lane 3:

Rat brain lysates at 20 µg

Lane 4:

Rat spleen lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 71 kDa

false

Western blot - Anti-GUCY1B3 antibody [EPR8822] (AB154841)

WB

Lab

Western blot - Anti-GUCY1B3 antibody [EPR8822] (AB154841)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-GUCY1B3 antibody [EPR8822] (ab154841) at 1/2000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse heart tissue lysate at 20 µg

Lane 3:

Mouse kidney tissue lysate at 20 µg

Lane 4:

Mouse spleen tissue lysate at 20 µg

Lane 5:

Rat brain tissue lysate at 20 µg

Lane 6:

Rat heart tissue lysate at 20 µg

Lane 7:

Rat kidney tissue lysate at 20 µg

Lane 8:

Rat spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 71 kDa

Observed band size: 65 kDa

false

Exposure time: 3min

Western blot - Anti-GUCY1B3 antibody [EPR8822] (AB154841)

WB

Unknown

Western blot - Anti-GUCY1B3 antibody [EPR8822] (AB154841)

All lanes:

Western blot - Anti-GUCY1B3 antibody [EPR8822] (ab154841) at 1/1000 dilution

Lane 1:

Human placenta lysate at 10 µg

Lane 2:

Human fetal brain lysate at 10 µg

Lane 3:

Human fetal lung lysate at 10 µg

Predicted band size: 71 kDa

false

Carrier free

Anti-GUCY1B3 antibody [EPR8822] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8822

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/400", "IHCP-species-notes": "<p><strong>For unpurified use at 1/100 -1/250. </strong></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GUCY1B3 also known as guanylate cyclase soluble subunit beta-1 serves as a subunit of the soluble guanylate cyclase (sGC) complex. This protein acts in converting GTP to cGMP which plays an important role in intracellular signaling. GUCY1B3 has a molecular mass of approximately 70 kDa. The expression of GUCY1B3 occurs in a variety of tissues with higher levels observed in smooth muscle cells and neurons.

Biological function summary

The GUCY1B3 protein functions as part of the soluble guanylate cyclase complex which directly responds to nitric oxide (NO) signaling. This NO binding leads to the production of cyclic GMP a secondary messenger involved in numerous biological responses. These responses include vasodilation and neurotransmission. GUCY1B3's activity regulates essential cellular processes through these mechanisms.

Pathways

The sGC complex involving GUCY1B3 participates in the nitric oxide signaling pathway which is significant for cardiovascular function. Additionally GUCY1B3 works alongside GUCY1A3 another subunit of the sGC complex to facilitate this signaling cascade. The resulting cGMP acts in downstream pathways like the cGMP-dependent protein kinase G (PKG) pathway which controls vascular tone and cardiac function.

GUCY1B3 plays a role in complications such as hypertension and pulmonary hypertension. Disruption in NO signaling involving GUCY1B3 can result in impaired vasodilation contributing to these conditions. Moreover alterations in the function of GUCY1A3 the partner in the sGC complex can affect the same pathways further implicating GUCY1B3 in the pathology of cardiovascular disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Mediates responses to nitric oxide (NO) by catalyzing the biosynthesis of the signaling molecule cGMP.

See full target information GUCY1B1

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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

World journal of gastrointestinal oncology 17:104522 PubMed40487942

2025

Simvastatin inhibits proliferation and migration, promotes oxidative stress and ferroptosis in colon cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Liu,Hao Ge,Zhi-Min Fan,Ting Lu,Lei He,Meng Li,Hao-Ran Zhao,Qiang Leng

The EMBO journal 43:1519-1544 PubMed38528180

2024

Pericyte signaling via soluble guanylate cyclase shapes the vascular niche and microenvironment of tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Zhu,Wu Yang,Jianyun Ma,Hao He,Zhen Liu,Xiaolan Zhu,Xueyang He,Jing He,Zhan Chen,Xiaoliang Jin,Xiaohong Wang,Kaiwen He,Wu Wei,Junhao Hu

Frontiers in pharmacology 14:1112123 PubMed36744261

2023

Stimulation of soluble guanylate cyclase by vericiguat reduces skeletal muscle atrophy of mice following chemotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Bo-Ang Hu,Yu-Lin Li,Hai-Tao Han,Bin Lu,Xu Jia,Lu Han,Wei-Xuan Ma,Ping Zhu,Zhi-Hao Wang,Wei Zhang,Ming Zhong,Lei Zhang

Physiological reports 11:e15549 PubMed36597221

2023

β3 adrenergic agonism: A novel pathway which improves right ventricular-pulmonary arterial hemodynamics in pulmonary arterial hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Keyvan Karimi Galougahi,Yunjia Zhang,Vivian Kienzle,Chia-Chi Liu,Lake-Ee Quek,Sanjay Patel,Edmund Lau,Rachael L Cordina,Gemma A Figtree,David S Celermajer

The Journal of experimental medicine 220: PubMed36350314

2022

Activating NO-sGC crosstalk in the mouse vascular niche promotes vascular integrity and mitigates acute lung injury.

Applications

Unspecified application

Species

Unspecified reactive species

Hao He,Wu Yang,Nan Su,Chuankai Zhang,Jianing Dai,Feng Han,Mahak Singhal,Wenjuan Bai,Xiaolan Zhu,Jing Zhu,Zhen Liu,Wencheng Xia,Xiaoting Liu,Chonghe Zhang,Kai Jiang,Wenhui Huang,Dan Chen,Zhaoyin Wang,Xueyang He,Frank Kirchhoff,Zhenyu Li,Cong Liu,Jingning Huan,Xiaohong Wang,Wu Wei,Jing Wang,Hellmut G Augustin,Junhao Hu

Nature communications 12:6652 PubMed34789728

2021

Soluble guanylate cyclase signalling mediates etoposide resistance in progressing small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Maximilian W Schenk,Sam Humphrey,A S Md Mukarram Hossain,Mitchell Revill,Sarah Pearsall,Alice Lallo,Stewart Brown,Samuel Bratt,Melanie Galvin,Tine Descamps,Cong Zhou,Simon P Pearce,Lynsey Priest,Michelle Greenhalgh,Anshuman Chaturvedi,Alastair Kerr,Fiona Blackhall,Caroline Dive,Kristopher K Frese

British journal of pharmacology 179:2490-2504 PubMed33963547

2021

Angiotensin II augments renal vascular smooth muscle soluble GC expression via an AT receptor-forkhead box subclass O transcription factor signalling axis.

Applications

Unspecified application

Species

Unspecified reactive species

Joseph C Galley,Scott A Hahn,Megan P Miller,Brittany G Durgin,Edwin K Jackson,Sean D Stocker,Adam C Straub

Cancers 12: PubMed33322158

2020

Dual Pharmacological Targeting of HDACs and PDE5 Inhibits Liver Disease Progression in a Mouse Model of Biliary Inflammation and Fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Alex Claveria-Cabello,Leticia Colyn,Iker Uriarte,Maria Ujue Latasa,Maria Arechederra,Jose M Herranz,Laura Alvarez,Jesus M Urman,Maria L Martinez-Chantar,Jesus M Banales,Bruno Sangro,Krista Rombouts,Julen Oyarzabal,Jose J G Marin,Carmen Berasain,Matias A Avila,Maite G Fernandez-Barrena

International journal of molecular sciences 21: PubMed32992747

2020

Multi-Compartment, Early Disruption of cGMP and cAMP Signalling in Cardiac Myocytes from the Model of Duchenne Muscular Dystrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Marcella Brescia,Ying-Chi Chao,Andreas Koschinski,Jakub Tomek,Manuela Zaccolo

JCI insight 5: PubMed31184598

2019

Conditional deletion of smooth muscle Cullin-3 causes severe progressive hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Larry N Agbor,Anand R Nair,Jing Wu,Ko-Ting Lu,Deborah R Davis,Henry L Keen,Frederick W Quelle,James A McCormick,Jeffrey D Singer,Curt D Sigmund

View all publications

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Product promise

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