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AB97669

Anti-GULP antibody

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(1 Publication)

Rabbit Polyclonal GULP antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human GULP1 aa 200 to C-terminus.

View Alternative Names

CED6, GULP, GULP1, PTB domain-containing engulfment adapter protein 1, Cell death protein 6 homolog, PTB domain adapter protein CED-6, Protein GULP

2 Images
Western blot - Anti-GULP antibody (AB97669)
  • WB

Unknown

Western blot - Anti-GULP antibody (AB97669)

10% SDS-PAGE.

All lanes:

Western blot - Anti-GULP antibody (ab97669) at 1/1000 dilution

Lane 1:

HepG2 whole cell lysate at 30 µg

Lane 2:

MOLT4 whole cell lysate at 30 µg

Predicted band size: 34 kDa

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Western blot - Anti-GULP antibody (AB97669)
  • WB

Unknown

Western blot - Anti-GULP antibody (AB97669)

10% SDS-PAGE.

All lanes:

Western blot - Anti-GULP antibody (ab97669) at 1/1000 dilution

All lanes:

NIH3T3 whole cell lysate at 30 µg

Predicted band size: 34 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human GULP1 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9UBP9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GULP also known as "CED-6" in some studies is a protein that contributes to several cellular processes. This protein has a molecular mass of approximately 34 kDa and demonstrates functional activity in various tissues although it most often appears in immune cells such as macrophages. GULP facilitates the engulfment and clearance of apoptotic cells by binding to internalizing receptors. These interactions are key for maintaining tissue homeostasis and preventing undesirable immune responses.
Biological function summary

The function of GULP extends beyond simple phagocytosis. It participates as part of a multi-protein complex that regulates cytoskeletal rearrangements necessary for cell movement and engulfment. This includes interactions with actin within the cells to drive membrane dynamics. The GULP protein is integral in coordinating intracellular signaling cascades that lead to efficient uptake and processing of cellular debris.

Pathways

The role of GULP is significant in the apoptotic cell clearance pathway. It regulates signals that engage with the Rho family of GTPases which modify cytoskeletal structures for phagocytosis. In addition GULP interacts with proteins like Ras-related C3 botulinum toxin substrate 1 (RAC1) another component in controlling actin cytoskeleton dynamics. These relationships place GULP strategically in the pathway networks that ensure cell renewal and clearance.

Defective function of GULP can contribute to autoimmune conditions such as systemic lupus erythematosus. In these diseases the failure to properly clear apoptotic cells may result in the production of autoantibodies against self-antigens. Another connection exists between GULP and cancer where impaired apoptotic cell clearance can facilitate the tumor microenvironment's evasion from immune surveillance involving proteins like MET tyrosine kinase receptor. These insights affirm the importance of understanding GULP's role in maintaining healthy cellular environments.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May function as an adapter protein. Required for efficient phagocytosis of apoptotic cells. Modulates cellular glycosphingolipid and cholesterol transport. May play a role in the internalization and endosomal trafficking of various LRP1 ligands, such as PSAP. Increases cellular levels of GTP-bound ARF6.
See full target information GULP1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular biology of the cell 25:1925-36 PubMed24743597

2014

The adaptor protein GULP promotes Jedi-1-mediated phagocytosis through a clathrin-dependent mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Chelsea S Sullivan,Jami L Scheib,Zhong Ma,Rajan P Dang,Johanna M Schafer,Francis E Hickman,Frances M Brodsky,Kodi S Ravichandran,Bruce D Carter
View all publications
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