AB114857

Anti-GW182 antibody

Name: Anti-GW182 antibody (ab114857) | Abcam
Brand: Abcam Limited
SKU: AB114857

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Polyclonal GW182 antibody. Suitable for IP and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human TNRC6A aa 1200-1300.

View Alternative Names

CAGH26, KIAA1460, TNRC6, TNRC6A, Trinucleotide repeat-containing gene 6A protein, CAG repeat protein 26, EMSY interactor protein, GW182 autoantigen, Glycine-tryptophan protein of 182 kDa, Protein GW1

1 Images

Unknown

Immunoprecipitation - Anti-GW182 antibody (AB114857)

Detection of GW182 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab114857 at 10 µg/mg lysate for IP (Lane 1). For WB detection an anti-GW182 antibody which recognizes a upstream epitope was used at 0.4 µg/ml. Lane 2 represents control IgG IP. Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-GW182 antibody (ab114857)

Predicted band size: 210 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human TNRC6A aa 1200-1300. The exact immunogen used to generate this antibody is proprietary information.

Q8NDV7

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "10 µg/mg of lysate", "IP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Chicken": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Turkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GW182 also known as TNRC6A serves a mechanical role as an important component in the microRNA-induced silencing complex (miRISC). This protein weighs about 182 kilodaltons and provides a platform for microRNA function. It is expressed in the cytoplasm of many cell types. GW182 interacts with Argonaute proteins which then assists in gene silencing by recruiting these proteins to mRNA targets.

Biological function summary

GW182 is involved in RNA-mediated post-transcriptional gene silencing. As part of the miRISC complex GW182 facilitates the repression or degradation of target mRNAs. This protein assists in binding with components such as PABP (poly(A)-binding protein) to regulate gene expression. This role influences cellular processes like development differentiation and response to stress.

Pathways

The actions of GW182 are linked to the RNA interference pathway and the microRNA-mediated gene silencing pathway. In these pathways GW182 collaborates closely with proteins like Argonaute (AGO) to execute its gene-silencing responsibilities. By addressing specific mRNA targets these interactions enable precise control of gene expression.

Irregularities in GW182 function connect to diseases involving dysregulated microRNA activity such as certain cancers and neurological disorders. Cancer involves aberrant expression of miRNAs which affects GW182's regulatory capacity. Another protein Dicer works alongside GW182 in generating functional miRNAs relevant in cancer pathways indicating how essential balance in this network maintains cellular health.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Plays a role in RNA-mediated gene silencing by both micro-RNAs (miRNAs) and short interfering RNAs (siRNAs). Required for miRNA-dependent repression of translation and for siRNA-dependent endonucleolytic cleavage of complementary mRNAs by argonaute family proteins. As a scaffolding protein, associates with argonaute proteins bound to partially complementary mRNAs, and can simultaneously recruit CCR4-NOT and PAN deadenylase complexes.

See full target information TNRC6A

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in physiology 8:482 PubMed28744224

2017

Downregulated Translation Initiation Signaling Predisposes Low-Birth-Weight Neonatal Pigs to Slower Rates of Muscle Protein Synthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Chen,Sydney R McCauley,Sally E Johnson,Robert P Rhoads,Samer W El-Kadi

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GW182 antibody

Immunoprecipitation - Anti-GW182 antibody (AB114857)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise