AB167407

Anti-H Cadherin antibody [EPR9621]

RabMAb
Recombinant
What is this?

5

(2 Reviews)

|

(6 Publications)

Rabbit Recombinant Monoclonal H Cadherin antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 6 publications.

View Alternative Names

CDHH, CDH13, Cadherin-13, Heart cadherin, P105, Truncated cadherin, H-cadherin, T-cad, T-cadherin

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H Cadherin antibody [EPR9621] (AB167407)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H Cadherin antibody [EPR9621] (AB167407)

ab167407 showing +ve staining in Human normal kidney.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H Cadherin antibody [EPR9621] (AB167407)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H Cadherin antibody [EPR9621] (AB167407)

ab167407 showing -ve staining in Human normal colon.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H Cadherin antibody [EPR9621] (AB167407)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H Cadherin antibody [EPR9621] (AB167407)

ab167407 showing -ve staining in Human normal liver.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H Cadherin antibody [EPR9621] (AB167407)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H Cadherin antibody [EPR9621] (AB167407)

Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling H Cadherin with ab167407 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H Cadherin antibody [EPR9621] (AB167407)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H Cadherin antibody [EPR9621] (AB167407)

ab167407 showing -ve staining in Human normal pancreas.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H Cadherin antibody [EPR9621] (AB167407)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H Cadherin antibody [EPR9621] (AB167407)

Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling H Cadherin with ab167407 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-H Cadherin antibody [EPR9621] (AB167407)

WB

Supplier Data

Western blot - Anti-H Cadherin antibody [EPR9621] (AB167407)

Double bands observed should be isoforms

All lanes:

Western blot - Anti-H Cadherin antibody [EPR9621] (ab167407) at 1/2195 dilution

All lanes:

Human Heart at 20 µg

Secondary

All lanes:

Goat anti-rabbit IgG (HRP) with minimal cross reactivity with human IgG at 1/2000 dilution

Predicted band size: 78 kDa

false

Exposure time: 5s

Carrier free

Anti-H Cadherin antibody [EPR9621] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9621

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

H Cadherin also known as Cadherin-13 or T-cadherin is a unique member of the cadherin family of cell adhesion molecules. It weighs approximately 95 kDa. Unlike classical cadherins H Cadherin lacks the transmembrane and cytoplasmic domains anchoring instead to the membrane via a glycosylphosphatidylinositol (GPI) anchor. H Cadherin expresses in various tissues including endothelial cells cardiac muscle and neurons suggesting its versatile role in different biological contexts.

Biological function summary

The protein plays a role in cell-cell interactions and tissue organization without forming typical adherens junctions. Researchers have identified H Cadherin as a part of the significant complex involved in neural network formation vascular patterning and cardiac tissue integrity. In neurons its expression modulates axon growth and guidance. In the cardiovascular system it contributes to heart function and blood vessel maintenance through its adhesive properties and involvement in signaling pathways.

Pathways

Several biological networks incorporate H Cadherin as an important element. Notably it participates in the Wnt signaling and PI3K/AKT pathways. H Cadherin interacts with proteins such as β-catenin in the Wnt pathway influencing cellular responses like proliferation and differentiation. In the PI3K/AKT pathway the protein may affect cell survival and metabolism. These interactions illustrate H Cadherin's involvement in important cellular processes.

H Cadherin has significant associations with cardiovascular diseases and certain cancers. Altered expression levels or mutations in H Cadherin can disrupt cellular adhesion and signaling contributing to the progression of atherosclerosis and tumor formation. In cancer linkages between H Cadherin and other cadherin family members like E-cadherin suggest complex interactions that can modulate tumor cell behavior influencing invasion and metastasis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. May act as a negative regulator of neural cell growth.

See full target information CDH13

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Open medicine (Warsaw, Poland) 19:20240904 PubMed38463522

2024

Serum levels of HMW adiponectin and its receptors are associated with cytokine levels and clinical characteristics in chronic obstructive pulmonary disease.

Applications

Unspecified application

Species

Unspecified reactive species

Li Lu,Mengyu Cheng

International journal of molecular sciences 24: PubMed37762507

2023

T-Cadherin Deficiency Is Associated with Increased Blood Pressure after Physical Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Vladimir S Popov,Ilya B Brodsky,Maria N Balatskaya,Alexander V Balatskiy,Ilia D Ozhimalov,Maria A Kulebyakina,Ekaterina V Semina,Mikhail S Arbatskiy,Viktoria S Isakova,Polina S Klimovich,Veronika Y Sysoeva,Natalia I Kalinina,Vsevolod A Tkachuk,Kseniya A Rubina

Head & neck 45:1162-1171 PubMed36939297

2023

High PD-L1 expression associates with low T-cadherin expression and poor prognosis in human papillomavirus-negative head and neck squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Qiuju Wang,Yanzhen Zhao,Yan Chen,Yibo Chen,Xiaoyu Song,Li Zhang,Qiao He,Bo Ye,Lichun Wu,Xinyue Huang,Dongsheng Wang

Stress (Amsterdam, Netherlands) 23:125-135 PubMed31347429

2019

Myocardial fibrosis, inflammation, and altered cardiac gene expression profiles in rats exposed to a predator-based model of posttraumatic stress disorder.

Applications

Unspecified application

Species

Unspecified reactive species

Boyd R Rorabaugh,Nathaniel W Mabe,Sarah L Seeley,Thorne S Stoops,Kasey E Mucher,Connor P Ney,Cassandra S Goodman,Brooke J Hertenstein,Austen E Rush,Charis D Kasler,Aaron M Sargeant,Phillip R Zoladz

Tissue engineering. Part C, Methods 25:168-175 PubMed30747044

2019

Optimization of CRISPR/Cas9 Technology to Knock Out Genes of Interest in Aneuploid Cell Lines.

Applications

Unspecified application

Species

Unspecified reactive species

Daniyar T Dyikanov,Petr A Vasiluev,Karina D Rysenkova,Natalia A Aleksandrushkina,Pyotr A Tyurin-Kuzmin,Konstantin Y Kulebyakin,Yury P Rubtsov,Anna A Shmakova,Maria N Evseeva,Alexander V Balatskiy,Ekaterina V Semina,Alexandra I Rostovtseva,Pavel I Makarevich,Maxim N Karagyaur

eLife 7: PubMed30475207

2018

FoxA1 and FoxA2 drive gastric differentiation and suppress squamous identity in NKX2-1-negative lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Soledad A Camolotto,Shrivatsav Pattabiraman,Timothy L Mosbruger,Alex Jones,Veronika K Belova,Grace Orstad,Mitchell Streiff,Lydia Salmond,Chris Stubben,Klaus H Kaestner,Eric L Snyder

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com