AB249422

Anti-H Cadherin antibody [EPR9621] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal H Cadherin antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.

View Alternative Names

CDHH, CDH13, Cadherin-13, Heart cadherin, P105, Truncated cadherin, H-cadherin, T-cad, T-cadherin

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H Cadherin antibody [EPR9621] - BSA and Azide free (AB249422)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H Cadherin antibody [EPR9621] - BSA and Azide free (AB249422)

This data was developed using ab167407, the same antibody clone in a different buffer formulation.

ab167407 showing +ve staining in Human normal kidney.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H Cadherin antibody [EPR9621] - BSA and Azide free (AB249422)

This data was developed using ab167407, the same antibody clone in a different buffer formulation.

ab167407 showing -ve staining in Human normal colon.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H Cadherin antibody [EPR9621] - BSA and Azide free (AB249422)

This data was developed using ab167407, the same antibody clone in a different buffer formulation.

ab167407 showing -ve staining in Human normal liver.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H Cadherin antibody [EPR9621] - BSA and Azide free (AB249422)

This data was developed using ab167407, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human brain tissue, labeling H Cadherin with ab167407 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H Cadherin antibody [EPR9621] - BSA and Azide free (AB249422)

This data was developed using ab167407, the same antibody clone in a different buffer formulation.

ab167407 showing -ve staining in Human normal pancreas.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H Cadherin antibody [EPR9621] - BSA and Azide free (AB249422)

This data was developed using ab167407, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling H Cadherin with ab167407 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-H Cadherin antibody [EPR9621] - BSA and Azide free (AB249422)

Double bands observed should be isoforms.

Lane 1:

Western blot - Anti-H Cadherin antibody [EPR9621] - BSA and Azide free (ab249422) at 1/1000 dilution

Lane 2:

Western blot - Anti-H Cadherin antibody [EPR9621] - BSA and Azide free (ab249422) at 1/5000 dilution

All lanes:

Human heart tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 78 kDa

false

Exposure time: 5s

Unconjugated

Anti-H Cadherin antibody [EPR9621]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9621

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

ab249422 is the carrier-free version of ab167407.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

H Cadherin also known as Cadherin-13 or T-cadherin is a unique member of the cadherin family of cell adhesion molecules. It weighs approximately 95 kDa. Unlike classical cadherins H Cadherin lacks the transmembrane and cytoplasmic domains anchoring instead to the membrane via a glycosylphosphatidylinositol (GPI) anchor. H Cadherin expresses in various tissues including endothelial cells cardiac muscle and neurons suggesting its versatile role in different biological contexts.

Biological function summary

The protein plays a role in cell-cell interactions and tissue organization without forming typical adherens junctions. Researchers have identified H Cadherin as a part of the significant complex involved in neural network formation vascular patterning and cardiac tissue integrity. In neurons its expression modulates axon growth and guidance. In the cardiovascular system it contributes to heart function and blood vessel maintenance through its adhesive properties and involvement in signaling pathways.

Pathways

Several biological networks incorporate H Cadherin as an important element. Notably it participates in the Wnt signaling and PI3K/AKT pathways. H Cadherin interacts with proteins such as β-catenin in the Wnt pathway influencing cellular responses like proliferation and differentiation. In the PI3K/AKT pathway the protein may affect cell survival and metabolism. These interactions illustrate H Cadherin's involvement in important cellular processes.

H Cadherin has significant associations with cardiovascular diseases and certain cancers. Altered expression levels or mutations in H Cadherin can disrupt cellular adhesion and signaling contributing to the progression of atherosclerosis and tumor formation. In cancer linkages between H Cadherin and other cadherin family members like E-cadherin suggest complex interactions that can modulate tumor cell behavior influencing invasion and metastasis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. May act as a negative regulator of neural cell growth.

See full target information CDH13

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-H Cadherin antibody [EPR9621] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H Cadherin antibody [EPR9621] - BSA and Azide free (AB249422)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H Cadherin antibody [EPR9621] - BSA and Azide free (AB249422)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H Cadherin antibody [EPR9621] - BSA and Azide free (AB249422)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H Cadherin antibody [EPR9621] - BSA and Azide free (AB249422)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H Cadherin antibody [EPR9621] - BSA and Azide free (AB249422)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H Cadherin antibody [EPR9621] - BSA and Azide free (AB249422)

Western blot - Anti-H Cadherin antibody [EPR9621] - BSA and Azide free (AB249422)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Assay Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise