AB45966

Anti-H-FABP antibody

(8 Reviews)

(13 Publications)

Rabbit Polyclonal H-FABP antibody. Suitable for WB and reacts with Recombinant full length protein - Human, Human samples. Cited in 13 publications.

View Alternative Names

FABP11, MDGI, FABP3, Fatty acid-binding protein 3, Heart-type fatty acid-binding protein, Mammary-derived growth inhibitor, Muscle fatty acid-binding protein, H-FABP, M-FABP

2 Images

Project3297****

Western blot - Anti-H-FABP antibody (AB45966)

ab45966 does not cross-react with small intestine or liver FABP.

All lanes:

Western blot - Anti-H-FABP antibody (ab45966) at 1 µg/mL

Lane 1:

Human liver tissue lysate - total protein (<a href='/en-us/products/unavailable/human-liver-tissue-lysate-total-protein-ab29889'>ab29889</a>) at 10 µg

Lane 2:

Human heart tissue lysate - total protein (<a href='/en-us/products/unavailable/human-heart-tissue-lysate-total-protein-ab29431'>ab29431</a>) at 10 µg

Lane 3:

Human skeletal muscle tissue lysate - total protein (<a href='/en-us/products/unavailable/human-skeletal-muscle-tissue-lysate-total-protein-ab29330'>ab29330</a>) at 10 µg

Lane 4:

Human small intestine tissue lysate - total protein (<a href='/en-us/products/unavailable/human-small-intestine-tissue-lysate-total-protein-ab29276'>ab29276</a>) at 10 µg

Secondary

All lanes:

Rabbit IgG secondary antibody (ab28446) at 1/10000 dilution

Predicted band size: 15 kDa

Observed band size: 15 kDa

false

Unknown

Western blot - Anti-H-FABP antibody (AB45966)

ab45966 recognizes the full length tagged recombinant protein (ab109160) which has an expected molecular weight of 16 kDa.

All lanes:

Western blot - Anti-H-FABP antibody (ab45966) at 1 µg/mL

All lanes:

Recombinant Human Cardiac FABP protein (<a href='/en-us/products/unavailable/recombinant-human-cardiac-fabp-protein-ab109160'>ab109160</a>) at 1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 15 kDa,22 kDa

true

Exposure time: 8min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

ab45966 reacts with H-FABP, but does not react with liver or intestinal forms of this protein.

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 98.98% PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

H-FABP also known as heart-type fatty acid binding protein is a small protein with a molecular mass of approximately 14.5 kDa. This protein is primarily found in the cytoplasm of cardiac muscle cells but it also can be detected in skeletal muscles and other tissues at lower concentrations. H-FABP binds long-chain fatty acids and is important for the intracellular transport and metabolism of these molecules. The protein is often used as a biomarker for cardiac injury due to its rapid release into the bloodstream following muscle damage.

Biological function summary

Fatty acid binding largely defines H-FABP's role facilitating the transport of fatty acids within cells which is key for energy production. It is not part of a larger protein complex but plays into broader lipid metabolism processes. The efficiency of H-FABP in fatty acid binding and transport helps maintain cellular energy homeostasis especially in tissues with high fatty acid metabolism like the heart.

Pathways

H-FABP integrates into the fatty acid metabolic pathways and the beta-oxidation pathway. It is associated with the transport and utilization of fatty acids linking it to the peroxisome proliferator-activated receptor (PPAR) signaling pathway an important regulator of lipid metabolism. Proteins such as CD36 and PPARα interact with H-FABP within these pathways influencing lipid storage and energy balance.

Disruptions in H-FABP levels or function correlate with cardiovascular diseases including myocardial infarction. Elevated blood levels of H-FABP provide early markers of cardiac cell injury. Additionally alterations can also relate to metabolic disorders like obesity where lipid metabolism gets impaired. In these contexts proteins such as troponin and creatine kinase may also serve as biomarkers elucidating the relationship between H-FABP and myocardial stress or damage.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

FABPs are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters.

See full target information FABP3

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Animal nutrition (Zhongguo xu mu shou yi xue hui) 13:126-136 PubMed37123620

2023

Sodium acetate promotes fat synthesis by suppressing TATA element modulatory factor 1 in bovine mammary epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Chaochao Luo,Nan Li,Qingzhu Wang,Chunjiang Li

Autophagy 17:2766-2782 PubMed33143524

2020

Autophagy receptor OPTN (optineurin) regulates mesenchymal stem cell fate and bone-fat balance during aging by clearing FABP3.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng-Zhao Liu,Chun-Gu Hong,Wen-Bao Hu,Meng-Lu Chen,Ran Duan,Hong-Ming Li,Tao Yue,Jia Cao,Zhen-Xing Wang,Chun-Yuan Chen,Xiong-Ke Hu,Ben Wu,Hao-Ming Liu,Yi-Juan Tan,Jiang-Hua Liu,Zhong-Wei Luo,Yan Zhang,Shan-Shan Rao,Ming-Jie Luo,Hao Yin,Yi-Yi Wang,Kun Xia,Lang Xu,Si-Yuan Tang,Rong-Gui Hu,Hui Xie

Frontiers in physiology 11:1090 PubMed32982800

2020

Inhibition of miR-27b Regulates Lipid Metabolism in Skeletal Muscle of Obese Rats During Hypoxic Exercise by Increasing PPARγ Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Xuebing Wang,Yingli Lu,Lei Zhu,Haibo Zhang,Lianshi Feng

The Journal of physiology 598:2469-2489 PubMed32338384

2020

Placental fatty acid transport across late gestation in a baboon model of intrauterine growth restriction.

Applications

Unspecified application

Species

Unspecified reactive species

Stephanie S Chassen,Veronique Ferchaud-Roucher,Claire Palmer,Cun Li,Thomas Jansson,Peter W Nathanielsz,Theresa L Powell

International journal of molecular sciences 20: PubMed31336948

2019

Hypoxia Modulates Effects of Fatty Acids on NES2Y Human Pancreatic β-cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jan Šrámek,Vlasta Němcová-Fürstová,Jan Polák,Jan Kovář

Biochemical and biophysical research communications 516:89-95 PubMed31200956

2019

New evidence of exercise training benefits in myostatin-deficient mice: Effect on lipidomic abnormalities.

Applications

Unspecified application

Species

Unspecified reactive species

Narjes Baati,Christine Feillet-Coudray,Gilles Fouret,Barbara Vernus,Bénédicte Goustard,Maxence Jollet,Christelle Bertrand-Gaday,Charles Coudray,Jérôme Lecomte,Anne Bonnieu,Christelle Koechlin-Ramonatxo

Cellular physiology and biochemistry : internation 52:94-108 PubMed30790507

2019

Linoleic Acid Increases Prostaglandin E2 Release and Reduces Mitochondrial Respiration and Cell Viability in Human Trophoblast-Like Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Nirajan Shrestha,James S M Cuffe,Olivia J Holland,Anthony V Perkins,Andrew J McAinch,Deanne H Hryciw

Journal of animal science and biotechnology 8:72 PubMed28883917

2017

Betaine affects muscle lipid metabolism via regulating the fatty acid uptake and oxidation in finishing pig.

Applications

Species

Pig

Sisi Li,Haichao Wang,Xinxia Wang,Yizhen Wang,Jie Feng

Biochimica et biophysica acta. Molecular and cell 1862:1044-1055 PubMed28676454

2017

Myostatin deficiency is associated with lipidomic abnormalities in skeletal muscles.

Applications

Species

Mouse

Narjes Baati,Christine Feillet-Coudray,Gilles Fouret,Barbara Vernus,Bénédicte Goustard,Charles Coudray,Jérome Lecomte,Véronique Blanquet,Laetitia Magnol,Anne Bonnieu,Christelle Koechlin-Ramonatxo

Scientific reports 7:2645 PubMed28572619

2017

Fatty Acid Binding Protein 3 And Transzonal Projections Are Involved In Lipid Accumulation During In Vitro Maturation Of Bovine Oocytes.

Applications

Species

Unspecified reactive species

Maite Del Collado,Juliano Coelho da Silveira,Juliano Rodrigues Sangalli,Gabriella Mamede Andrade,Letícia Rabello da Silva Sousa,Luciano Andrade Silva,Flavio Vieira Meirelles,Felipe Perecin

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