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AB133585

Anti-H-FABP antibody [EPR6126]

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(7 Publications)

Rabbit Recombinant Monoclonal H-FABP antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 7 publications.

View Alternative Names

FABP11, MDGI, FABP3, Fatty acid-binding protein 3, Heart-type fatty acid-binding protein, Mammary-derived growth inhibitor, Muscle fatty acid-binding protein, H-FABP, M-FABP

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H-FABP antibody [EPR6126] (AB133585)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H-FABP antibody [EPR6126] (AB133585)

Immunohistochemical analysis of paraffin embedded Human heart tissue labelling H-FABP with ab133585 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-H-FABP antibody [EPR6126] (AB133585)
  • WB

Unknown

Western blot - Anti-H-FABP antibody [EPR6126] (AB133585)

All lanes:

Western blot - Anti-H-FABP antibody [EPR6126] (ab133585) at 1/1000 dilution

Lane 1:

Fetal muscle tissue lysate at 10 µg

Lane 2:

Fetal heart tissue lysate at 10 µg

Secondary

All lanes:

HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

Predicted band size: 15 kDa

false

OI-RD Scanning - Anti-H-FABP antibody [EPR6126] (AB133585)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-H-FABP antibody [EPR6126] (AB133585)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Carrier free

    Anti-H-FABP antibody [EPR6126] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6126

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

H-FABP also known as heart-type fatty acid binding protein is a small protein with a molecular mass of approximately 14.5 kDa. This protein is primarily found in the cytoplasm of cardiac muscle cells but it also can be detected in skeletal muscles and other tissues at lower concentrations. H-FABP binds long-chain fatty acids and is important for the intracellular transport and metabolism of these molecules. The protein is often used as a biomarker for cardiac injury due to its rapid release into the bloodstream following muscle damage.
Biological function summary

Fatty acid binding largely defines H-FABP's role facilitating the transport of fatty acids within cells which is key for energy production. It is not part of a larger protein complex but plays into broader lipid metabolism processes. The efficiency of H-FABP in fatty acid binding and transport helps maintain cellular energy homeostasis especially in tissues with high fatty acid metabolism like the heart.

Pathways

H-FABP integrates into the fatty acid metabolic pathways and the beta-oxidation pathway. It is associated with the transport and utilization of fatty acids linking it to the peroxisome proliferator-activated receptor (PPAR) signaling pathway an important regulator of lipid metabolism. Proteins such as CD36 and PPARα interact with H-FABP within these pathways influencing lipid storage and energy balance.

Disruptions in H-FABP levels or function correlate with cardiovascular diseases including myocardial infarction. Elevated blood levels of H-FABP provide early markers of cardiac cell injury. Additionally alterations can also relate to metabolic disorders like obesity where lipid metabolism gets impaired. In these contexts proteins such as troponin and creatine kinase may also serve as biomarkers elucidating the relationship between H-FABP and myocardial stress or damage.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

FABPs are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters.
See full target information FABP3
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular and molecular medicine 27:2956-2969 PubMed37654004

2023

Effects of early exercise on cardiac function and lipid metabolism pathway in heart failure.

Applications

Unspecified application

Species

Unspecified reactive species

Sérgio Luiz Borges de Souza,Gustavo Augusto Ferreira Mota,Vitor Loureiro da Silva,Danielle Fernandes Vileigas,Paula Grippa Sant'Ana,Cristina Schmitt Gregolin,Rebeca Lopes Figueira,Sabrina Setembre Batah,Alexandre Todorovic Fabro,Gilson Masahiro Murata,Silmeia Garcia Zanati Bazan,Marina Politi Okoshi,Antonio Carlos Cicogna

Scientific reports 13:2933 PubMed36806358

2023

Molecular mechanism of valine and its metabolite in improving triglyceride synthesis of porcine intestinal epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mengmeng Xu,Long Che,Lizhu Niu,Liuzhen Wang,Mengyun Li,Dongfeng Jiang,Hongyu Deng,Wen Chen,Zongyong Jiang

Cancer communications (London, England) 42:287-313 PubMed35184419

2022

Essential roles of exosome and circRNA_101093 on ferroptosis desensitization in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Zhang,Yunhua Xu,Lifang Ma,Keke Yu,Yongjie Niu,Xin Xu,Yi Shi,Susu Guo,Xiangfei Xue,Yikun Wang,Shiyu Qiu,Jiangtao Cui,Hong Wang,Xiaoting Tian,Yayou Miao,Fanyu Meng,Yongxia Qiao,Yongchun Yu,Jiayi Wang

Scientific reports 9:18050 PubMed31792287

2019

Landscape of heart proteome changes in a diet-induced obesity model.

Applications

Unspecified application

Species

Unspecified reactive species

Danielle F Vileigas,Victoria M Harman,Paula P Freire,Cecília L C Marciano,Paula G Sant'Ana,Sérgio L B de Souza,Gustavo A F Mota,Vitor L da Silva,Dijon H S Campos,Carlos R Padovani,Katashi Okoshi,Robert J Beynon,Lucilene D Santos,Antonio C Cicogna

Biology of reproduction 101:126-137 PubMed30985894

2019

Valine increases milk fat synthesis in mammary gland of gilts through stimulating AKT/MTOR/SREBP1 pathway†.

Applications

Unspecified application

Species

Unspecified reactive species

Long Che,Mengmeng Xu,Kaiguo Gao,Cui Zhu,Li Wang,Xuefen Yang,Xiaolu Wen,Hao Xiao,Zongyong Jiang,De Wu

Journal of cellular biochemistry 118:1316-1329 PubMed27859593

2017

Placental-Specific Overexpression of sFlt-1 Alters Trophoblast Differentiation and Nutrient Transporter Expression in an IUGR Mouse Model.

Applications

Unspecified application

Species

Unspecified reactive species

Elisabeth Kühnel,Veronika Kleff,Violeta Stojanovska,Stephanie Kaiser,Ralph Waldschütz,Florian Herse,Torsten Plösch,Elke Winterhager,Alexandra Gellhaus

Journal of cellular biochemistry 117:1594-603 PubMed26590355

2015

Increased Rat Placental Fatty Acid, but Decreased Amino Acid and Glucose Transporters Potentially Modify Intrauterine Programming.

Applications

Unspecified application

Species

Unspecified reactive species

Eva Nüsken,Alexandra Gellhaus,Elisabeth Kühnel,Isabelle Swoboda,Maria Wohlfarth,Christina Vohlen,Holm Schneider,Jörg Dötsch,Kai-Dietrich Nüsken
View all publications
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Product promise

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