JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB156763

Anti-H-protein antibody [OTI3G1]

1

(1 Review)

|

(1 Publication)

Mouse Monoclonal H-protein antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, African green monkey samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MYBPH.

View Alternative Names

Myosin-binding protein H, MyBP-H, H-protein, MYBPH

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H-protein antibody [OTI3G1] (AB156763)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H-protein antibody [OTI3G1] (AB156763)

Paraffin embedded Human lung carcinoma tissue labeling H-protein with ab156763 at 1/150 in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H-protein antibody [OTI3G1] (AB156763)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H-protein antibody [OTI3G1] (AB156763)

Paraffin embedded Human pancreas tissue labeling H-protein with ab156763 at 1/150 in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H-protein antibody [OTI3G1] (AB156763)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H-protein antibody [OTI3G1] (AB156763)

Paraffin embedded Human colon adenocarcinoma tissue labeling H-protein with ab156763 at 1/150 in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-H-protein antibody [OTI3G1] (AB156763)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-H-protein antibody [OTI3G1] (AB156763)

Immunofluorescence analysis of COS7 cells transiently transfected by pCMV6-ENTRY H-protein labeling H-protein with ab156763 at 1/100.

Western blot - Anti-H-protein antibody [OTI3G1] (AB156763)
  • WB

Unknown

Western blot - Anti-H-protein antibody [OTI3G1] (AB156763)

All lanes:

Western blot - Anti-H-protein antibody [OTI3G1] (ab156763) at 1/4000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control at 5 µg

Lane 2:

HEK-293T cells transfected with pCMV6-ENTRY H-protein at 5 µg

Predicted band size: 52 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI3G1

Isotype

IgG2a

Carrier free

No

Reacts with

Human, African green monkey

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human MYBPH.

Q13203

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/4000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "African green monkey": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" } } }
style
left-column

Product details

The clone number has been updated from 3G1 to OTI3G1, both clone numbers name the same clone.
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The H-protein also known as GCSh (Glycine Cleavage System H) protein plays an important role in the glycine cleavage system. It has a molecular mass of approximately 14 kDa. This protein is expressed in various tissues with significant expression in the liver and kidneys. Mechanically H-protein functions as a carrier protein facilitating the transfer of a methylamine group during the glycine decarboxylation process. This action is important in the conversion of glycine a non-essential amino acid to carbon dioxide ammonia NADH and methylene tetrahydrofolate.
Biological function summary

The function of H-protein is essential for cellular energy production and provision of one-carbon units for metabolism. H-protein is part of the glycine cleavage complex which includes P-protein T-protein and L-protein. This complex collectively breaks down glycine an important step for energy generation and maintaining low levels of toxic glycine in mitochondria. This process also contributes to the pool of one-carbon units necessary for various biosynthetic pathways impacting nucleotide synthesis and methylation reactions.

Pathways

The glycine cleavage system which includes H-protein is integrated into two key biological pathways: amino acid metabolism and mitochondrial energy production. In amino acid metabolism it collaborates with other proteins like P-protein to facilitate the breakdown of glycine thereby regulating levels of this amino acid. In mitochondrial energy production the activity of H-protein ensures the removal of excess glycine which can affect mitochondrial function and energy balance if accumulated. This dynamic further connects it to wider metabolic cycles impacting overall cellular health and efficiency.

Disruptions in H-protein function are linked to non-ketotic hyperglycinemia and possibly a few cases of metabolic disorders involving glycine. Non-ketotic hyperglycinemia results from mutations affecting the glycine cleavage system including defective activities in GCS-related proteins like P-protein which leads to elevated glycine levels and neurological issues. Studies also suggest that altered H-protein activity may contribute to other mitochondrial disorders as its efficient function is important for maintaining mitochondrial health thereby indirectly connecting with proteins involved in mitochondrial dynamics and metabolism.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Binds to myosin; probably involved in interaction with thick myofilaments in the A-band.
See full target information MYBPH
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 12:450 PubMed33958582

2021

Single cell transcriptional zonation of human psoriasis skin identifies an alternative immunoregulatory axis conducted by skin resident cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yuge Gao,Xinyu Yao,Yumeng Zhai,Li Li,Huini Li,Xianqi Sun,Pei Yu,Tiankuo Xue,Yuzhen Li,Yizhou Hu
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB22048

Anti-TLR4 antibody [76B357.1]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR4 antibody [76B357.1] (AB22048)

  • 4
  • 29
Primary Antibodies

AB241578

Anti-UTP18 antibody

Immunoprecipitation - Anti-UTP18 antibody (AB241578)

  • 0
  • 0
Primary Antibodies

AB240699

Anti-ZNF592 antibody

Immunoprecipitation - Anti-ZNF592 antibody (AB240699)

  • 0
  • 0
Primary Antibodies

AB236428

Anti-ZNF558 antibody [OTI2C4]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF558 antibody [OTI2C4] (AB236428)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com