AB104870

Anti-H1N1 Influenza A virus Nucleocapsid protein antibody

Name: Anti-H1N1 Influenza A virus Nucleocapsid protein antibody (ab104870) | Abcam
Brand: Abcam Limited
SKU: AB104870
Price: 525 USD
Availability: InStock

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(16 Publications)

Rabbit Polyclonal NCAP antibody. Suitable for ELISA, WB, ICC/IF and reacts with Influenza A samples. Cited in 16 publications.

View Alternative Names

Nucleoprotein, Nucleocapsid protein, Protein N, NP

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Influenza A

Applications

WB, ICC/IF, ELISA

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Influenza A": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "Dilute 1:200 - 1:2000", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purity

Whole antiserum

Storage buffer

Constituents: Whole serum

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The H1N1 Influenza A virus nucleocapsid protein also known as the nucleoprotein (NP) plays a central role in the encapsidation of the viral RNA genome. With a molecular mass of approximately 56 kDa NP forms ribonucleoprotein (RNP) complexes that are essential in the virus's replication and transcription processes. The NP is expressed in the cytoplasm of infected host cells where it binds to viral RNA facilitating the formation of helical RNP structures. These structures are critical for the stability and function of the viral genome.

Biological function summary

The NP participates in the virus life cycle by enabling RNA synthesis and interacting with other viral proteins. As part of the RNP complex it collaborates with the RNA polymerase complex made up of PA PB1 and PB2 proteins. This interaction is essential for the transcription and replication of viral RNA. Additionally NP is known to activate host cell signaling pathways that modulate immune response helping the virus evade immune detection and destruction.

Pathways

The NP protein interacts mainly with the viral replication and transcription mechanisms. The replication-transcription pathway features the synchronization of NP with the RNA polymerase complex ensuring the efficient synthesis of new viral genome copies. The NP also interacts with signaling pathways that involve host proteins like importins which regulate the nuclear import of the RNP complex important for its function.

NP is intimately associated with influenza outbreaks especially those caused by the H1N1 flu virus. This protein's interaction with host immune pathways can alter the immune response contributing to the virus's pathogenicity. Its relation to influenza is also marked by its role in viral replication where it works alongside the M1 matrix protein to export progeny RNPs from the nucleus. Understanding NP is important in the development of flu antibodies as it presents a valuable target for vaccines and antiviral treatments against H1N1 influenza.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the host nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals that are responsible for the active RNP import into the nucleus through cellular importin alpha/beta pathway. Later in the infection, nclear export of RNPs are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that nucleoprotein binds directly host exportin-1/XPO1 and plays an active role in RNPs nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmasks nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus.

See full target information NP

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Publications (16)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed39941149

2025

Discovery of TRPV4-Targeting Small Molecules with Anti-Influenza Effects Through Machine Learning and Experimental Validation.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Sun,Jiajing Wu,Beilei Shen,Hengzheng Yang,Huizi Cui,Weiwei Han,Rongbo Luo,Shijun Zhang,He Li,Bingshuo Qian,Lingjun Fan,Junkui Zhang,Tiecheng Wang,Xianzhu Xia,Fang Yan,Yuwei Gao

Journal of medical virology 96:e29906 PubMed39262090

2024

Broad-spectrum antiviral effect of MoringaA-loaded exosomes against IAV by mediating the GCN5-TFEB-autolysosome pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Chunmei Lv,Ruidong Li,Dandan Yang,Shunqiang Song,Xu Cheng,Tingting Chen,Lei Chen,Yongai Xiong

International journal of nanomedicine 19:7399-7414 PubMed39071500

2024

Covalent Organic Framework-Based Theranostic Platforms for Restricting H1N1 Influenza Virus Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Luo-Gang Ding,Xiang Ji,Yue-Yue Liu,Min Shi,Jian-Da Li,Fei Liu,Yu-Yu Zhang,Jiang Yu,Jia-Qiang Wu

Nature communications 14:8398 PubMed38110357

2023

Intranasal mask for protecting the respiratory tract against viral aerosols.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoming Hu,Shuang Wang,Shaotong Fu,Meng Qin,Chengliang Lyu,Zhaowen Ding,Yan Wang,Yishu Wang,Dongshu Wang,Li Zhu,Tao Jiang,Jing Sun,Hui Ding,Jie Wu,Lingqian Chang,Yimin Cui,Xiaocong Pang,Youchun Wang,Weijin Huang,Peidong Yang,Limin Wang,Guanghui Ma,Wei Wei

Chinese medicine 18:144 PubMed37919750

2023

A potential Chinese medicine monomer against influenza A virus and influenza B virus: isoquercitrin.

Applications

Unspecified application

Species

Unspecified reactive species

Rongbo Luo,Chaoxiang Lv,Tiecheng Wang,Xiuwen Deng,Mingwei Sima,Jin Guo,Jing Qi,Weiyang Sun,Beilei Shen,Yuanguo Li,Donghui Yue,Yuwei Gao

iScience 26:107698 PubMed37680489

2023

Viral sensing by epithelial cells involves PKR- and caspase-3-dependent generation of gasdermin E pores.

Applications

Unspecified application

Species

Unspecified reactive species

Coralie Guy,Marcin Baran,Pau Ribó-Molina,Bernadette G van den Hoogen,Andrew G Bowie

International journal of obesity (2005) 47:1088-1099 PubMed37587162

2023

Thoracic adipose tissue contributes to severe virus infection of the lung.

Applications

Unspecified application

Species

Unspecified reactive species

Franziska Hornung,Luise Schulz,Nilay Köse-Vogel,Antje Häder,Jana Grießhammer,Daniel Wittschieber,Angelina Autsch,Christina Ehrhardt,Gita Mall,Bettina Löffler,Stefanie Deinhardt-Emmer

International journal of molecular sciences 24: PubMed36768720

2023

Inhibition of EZH2 Causes Retrotransposon Derepression and Immune Activation in Porcine Lung Alveolar Macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Liangliang Zhang,Jian Jin,Weiyun Qin,Jing Jiang,Wenbin Bao,Ming-An Sun

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 9:e2204246 PubMed36253095

2022

A Versatile Biomimic Nanotemplating Fluidic Assay for Multiplex Quantitative Monitoring of Viral Respiratory Infections and Immune Responses in Saliva and Blood.

Applications

Unspecified application

Species

Unspecified reactive species

Roozbeh Siavash Moakhar,Carolina Del Real Mata,Mahsa Jalali,Houda Shafique,Alireza Sanati,Justin de Vries,Julia Strauss,Tamer AbdElFatah,Fahimeh Ghasemi,Myles McLean,Imman I Hosseini,Yao Lu,Sripadh Guptha Yedire,Sahar Sadat Mahshid,Mohammad Amin Tabatabaiefar,Chen Liang,Sara Mahshid

Scientific reports 12:8515 PubMed35595813

2022

ILDR1 promotes influenza A virus replication through binding to PLSCR1.

Applications

Unspecified application

Species

Unspecified reactive species

Yueyue Liu,Shuqian Lin,Yunhui Xie,Lu Zhao,Haibo Du,Shifa Yang,Bin Yin,Guiming Li,Zengcheng Zhao,Zhongli Huang,Zhigang Xu,Jiaqiang Wu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-H1N1 Influenza A virus Nucleocapsid protein antibody

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Reactivity data

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (16)

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