AB39964

Anti-H2R antibody

Name: Anti-H2R antibody (ab39964) | Abcam
Brand: Abcam Limited
SKU: AB39964
Price: 525 USD
Availability: InStock

Be the first to review this product! Submit a review

(1 Publication)

Goat Polyclonal H2R antibody. Suitable for WB, Flow Cyt, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human Histamine H2 receptor aa 300-350.

View Alternative Names

Histamine H2 receptor, H2R, HH2R, Gastric receptor I, HRH2

3 Images

Flow Cytometry - Anti-H2R antibody (AB39964)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-H2R antibody (AB39964)

Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (1µg/mL). IgG control : Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-H2R antibody (AB39964)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-H2R antibody (AB39964)

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control : Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Supplier Data

Western blot - Anti-H2R antibody (AB39964)

Detected by chemiluminescence.

All lanes:

Western blot - Anti-H2R antibody (ab39964) at 1 µg/mL

All lanes:

Human tonsil tissue lysates (35µg protein in RIPA buffer)

Predicted band size: 40 kDa

false

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt, ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human Histamine H2 receptor aa 300-350. The exact immunogen used to generate this antibody is proprietary information.

P25021

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "A 1 hour primary incubation is recommended for this product.", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "10 µg/mL", "FlowCyt-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.5% BSA, 0.5% Tris buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

H2R also known as histamine H2 receptor is a G-protein-coupled receptor with a mass of approximately 40 kDa. It is expressed mainly in the gastric parietal cells as well as in the heart brain and various immune cells. H2R modulates gastric acid secretion by engaging in the interaction with histamine which activates the receptor. This activation includes changes in intracellular cyclic AMP levels and subsequent cellular responses.

Biological function summary

The histamine H2 receptor plays a significant role in the mediation of gastric acid secretion cardiac muscle contraction and immune response modulation. Although it functions independently it does interact with other receptors giving a context like mast cells which secrete histamine. These receptors play an important role in stimulating gastric parietal cells to produce acid as well as having involvement in the modulation of heart rate and force of contraction.

Pathways

H2R participates in the GPCR signaling pathway and adenylate cyclase activation both of which are critical for physiological responses in gastric acid secretion and modulation of cardiac function. H2R interacts with proteins such as adenylate cyclase and other H receptors like H1 receptor contributing to the regulation of varied responses across tissues like the stomach heart and brain.

H2R is strongly related to peptic ulcers and gastroesophageal reflux disease (GERD). In these conditions excessive secretion of gastric acid due to H2R overactivity can lead to mucosal damage and discomfort. Moreover other histamine receptors like H1 receptor might influence inflammatory processes and allergy drawing connections that are relevant to understanding histamine's overall impacts in the body.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

G-protein coupled receptor for histamine, primarily mediating gastric acid secretion. Predominantly expressed in the gastric mucosa, couples to G(s) G alpha proteins upon histamine binding, leading to activation of adenylate cyclase and increased intracellular cyclic AMP (cAMP) levels (PubMed : 38647423, PubMed : 39333117). This signaling cascade stimulates parietal cells to secrete hydrochloric acid, playing a key role in digestive physiology. Also expressed in other tissues, including the heart and central nervous system, where it may contribute to cardiac stimulation and modulate neurotransmitter release (By similarity).

See full target information Histamine H2 receptor

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

PloS one 17:e0277956 PubMed36525420

2022

Anti-GRP-R monoclonal antibody antitumor therapy against neuroblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jingbo Qiao,Junquan Liu,Jillian C Jacobson,Rachael A Clark,Sora Lee,Li Liu,Zhiqiang An,Ningyan Zhang,Dai H Chung

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-H2R antibody

Flow Cytometry - Anti-H2R antibody (AB39964)

Immunocytochemistry/ Immunofluorescence - Anti-H2R antibody (AB39964)

Western blot - Anti-H2R antibody (AB39964)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise