Goat Polyclonal H2R antibody. Suitable for WB, Flow Cyt, ICC/IF and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human Histamine H2 receptor aa 300-350.
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Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human Histamine H2 receptor aa 300-350. The exact immunogen used to generate this antibody is proprietary information. Database link P25021
Consider this alternative
Reactivity data
Select an application| WB | Flow Cyt | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Dog | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes A 1 hour primary incubation is recommended for this product. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Dog | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Dog | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Dog | Dilution info - | Notes - |
Associated Products
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Target data
Function
The H2 subclass of histamine receptors mediates gastric acid secretion. Also appears to regulate gastrointestinal motility and intestinal secretion. Possible role in regulating cell growth and differentiation. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and, through a separate G protein-dependent mechanism, the phosphoinositide/protein kinase (PKC) signaling pathway (By similarity).
Alternative names
Histamine H2 receptor, H2R, HH2R, Gastric receptor I, HRH2
Recommended products
Goat Polyclonal H2R antibody. Suitable for WB, Flow Cyt, ICC/IF and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human Histamine H2 receptor aa 300-350.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human Histamine H2 receptor aa 300-350. The exact immunogen used to generate this antibody is proprietary information. Database link P25021
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
H2R also known as histamine H2 receptor is a G-protein-coupled receptor with a mass of approximately 40 kDa. It is expressed mainly in the gastric parietal cells as well as in the heart brain and various immune cells. H2R modulates gastric acid secretion by engaging in the interaction with histamine which activates the receptor. This activation includes changes in intracellular cyclic AMP levels and subsequent cellular responses.
- Biological function summary
The histamine H2 receptor plays a significant role in the mediation of gastric acid secretion cardiac muscle contraction and immune response modulation. Although it functions independently it does interact with other receptors giving a context like mast cells which secrete histamine. These receptors play an important role in stimulating gastric parietal cells to produce acid as well as having involvement in the modulation of heart rate and force of contraction.
- Pathways
H2R participates in the GPCR signaling pathway and adenylate cyclase activation both of which are critical for physiological responses in gastric acid secretion and modulation of cardiac function. H2R interacts with proteins such as adenylate cyclase and other H receptors like H1 receptor contributing to the regulation of varied responses across tissues like the stomach heart and brain.
- Associated diseases and disorders
H2R is strongly related to peptic ulcers and gastroesophageal reflux disease (GERD). In these conditions excessive secretion of gastric acid due to H2R overactivity can lead to mucosal damage and discomfort. Moreover other histamine receptors like H1 receptor might influence inflammatory processes and allergy drawing connections that are relevant to understanding histamine's overall impacts in the body.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-H2R antibody (ab39964)
Detected by chemiluminescence.
All lanes: Western blot - Anti-H2R antibody (ab39964) at 1 µg/mL
All lanes: Human tonsil tissue lysates (35µg protein in RIPA buffer)
Predicted band size: 40 kDa
Immunocytochemistry/ Immunofluorescence - Anti-H2R antibody (ab39964)
Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Flow Cytometry - Anti-H2R antibody (ab39964)
Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (1µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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