AB108540

Anti-H2R antibody [EPR5630]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal H2R antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Histamine H2 receptor, H2R, HH2R, Gastric receptor I, HRH2

1 Images

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Western blot - Anti-H2R antibody [EPR5630] (AB108540)

All lanes:

Western blot - Anti-H2R antibody [EPR5630] (ab108540) at 1/10000 dilution

Lane 1:

Human fetal brain lysate at 10 µg

Lane 2:

SH-SY5Y cell lysate at 10 µg

Lane 3:

Human adrenal gland lysate at 10 µg

Predicted band size: 40 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5630

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

H2R also known as histamine H2 receptor is a G-protein-coupled receptor with a mass of approximately 40 kDa. It is expressed mainly in the gastric parietal cells as well as in the heart brain and various immune cells. H2R modulates gastric acid secretion by engaging in the interaction with histamine which activates the receptor. This activation includes changes in intracellular cyclic AMP levels and subsequent cellular responses.

Biological function summary

The histamine H2 receptor plays a significant role in the mediation of gastric acid secretion cardiac muscle contraction and immune response modulation. Although it functions independently it does interact with other receptors giving a context like mast cells which secrete histamine. These receptors play an important role in stimulating gastric parietal cells to produce acid as well as having involvement in the modulation of heart rate and force of contraction.

Pathways

H2R participates in the GPCR signaling pathway and adenylate cyclase activation both of which are critical for physiological responses in gastric acid secretion and modulation of cardiac function. H2R interacts with proteins such as adenylate cyclase and other H receptors like H1 receptor contributing to the regulation of varied responses across tissues like the stomach heart and brain.

H2R is strongly related to peptic ulcers and gastroesophageal reflux disease (GERD). In these conditions excessive secretion of gastric acid due to H2R overactivity can lead to mucosal damage and discomfort. Moreover other histamine receptors like H1 receptor might influence inflammatory processes and allergy drawing connections that are relevant to understanding histamine's overall impacts in the body.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

G-protein coupled receptor for histamine, primarily mediating gastric acid secretion. Predominantly expressed in the gastric mucosa, couples to G(s) G alpha proteins upon histamine binding, leading to activation of adenylate cyclase and increased intracellular cyclic AMP (cAMP) levels (PubMed : 38647423, PubMed : 39333117). This signaling cascade stimulates parietal cells to secrete hydrochloric acid, playing a key role in digestive physiology. Also expressed in other tissues, including the heart and central nervous system, where it may contribute to cardiac stimulation and modulate neurotransmitter release (By similarity).

See full target information Histamine H2 receptor

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:1312 PubMed40885864

2025

A single-cell atlas of mouse central nervous system immune cells reveals unique infection-stage immune signatures during the progression of meningitis caused by Streptococcus suis.

Applications

Unspecified application

Species

Unspecified reactive species

Xuan Jiang,Jikun Mei,Junhui Zhu,Yanyan Tian,Tong Wu,Ziheng Li,Zengshuai Wu,Tamim Abdelaal,Fengyang Li,Na Li,Liancheng Lei

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-H2R antibody [EPR5630]

Western blot - Anti-H2R antibody [EPR5630] (AB108540)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise