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AB170895

Anti-H6PD/GDH antibody [EPR12338(B)]

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(13 Publications)

Rabbit Recombinant Monoclonal H6PD/GDH antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 13 publications.

View Alternative Names

GDH, H6PD, GDH/6PGL endoplasmic bifunctional protein

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H6PD/GDH antibody [EPR12338(B)] (AB170895)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H6PD/GDH antibody [EPR12338(B)] (AB170895)

Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling H6PD/GDH with ab170895 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H6PD/GDH antibody [EPR12338(B)] (AB170895)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H6PD/GDH antibody [EPR12338(B)] (AB170895)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling H6PD/GDH with ab170895 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-H6PD/GDH antibody [EPR12338(B)] (AB170895)
  • WB

Supplier Data

Western blot - Anti-H6PD/GDH antibody [EPR12338(B)] (AB170895)

All lanes:

Western blot - Anti-H6PD/GDH antibody [EPR12338(B)] (ab170895) at 1/1000 dilution

Lane 1:

Fetal liver lysate at 10 µg

Lane 2:

MCF-7 lysate at 10 µg

Lane 3:

Rat liver lysate at 10 µg

Lane 4:

HepG2 lysate at 10 µg

Lane 5:

Mouse liver lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 89 kDa

false

  • Carrier free

    Anti-H6PD/GDH antibody [EPR12338(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12338(B)

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Secondary Antibodies

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Primary Antibodies

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Primary Antibodies

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Primary Antibodies

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  • 5
  • 104
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

H6PD also known as glucose dehydrogenase (GDH) or G.D.H. is an enzyme with an important mechanical role in cellular metabolism. It has a molecular mass of approximately 92 kDa. This enzyme participates in the reversible oxidation of glucose and other hexose sugars an action that results in the generation of NADPH. H6PD is expressed mainly in the endoplasmic reticulum of various tissues including liver adrenal gland and adipose tissue where it facilitates the pentose phosphate pathway and other metabolic functions.
Biological function summary

H6PD influences multiple metabolic processes by maintaining cellular redox balance through NADPH production. It plays a role in carbohydrate metabolism and aids in lipid biosynthesis and detoxification processes. Although H6PD does not form a complex it works closely with 11β-hydroxysteroid dehydrogenase type 1 an enzyme involved in corticosteroid metabolism. This interaction supports the regulation of local cortisol concentrations via the interconversion of active and inactive forms.

Pathways

Several metabolic pathways integrate the function of H6PD including the pentose phosphate pathway and the gluconeogenesis pathway. In the pentose phosphate pathway H6PD contributes to the generation of ribose-5-phosphate a precursor for nucleotide synthesis. Related proteins like glucose-6-phosphate dehydrogenase (G6PD) also share roles in these pathways working together to ensure the proper supply of reducing agents like NADPH needed for anabolic reactions.

H6PD has associations with metabolic disorders such as cortisone reductase deficiency and type 2 diabetes. Its role in cortisone metabolism connects it closely with 11β-hydroxysteroid dehydrogenase type 1 which can lead to deregulation of corticosteroid activity and contribute to insulin resistance and obesity. Understanding the connections between H6PD function and metabolic syndrome aids in revealing the complex landscape of metabolic control and its clinical implications.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Bifunctional enzyme localized in the lumen of the endoplasmic reticulum that catalyzes the first two steps of the oxidative branch of the pentose phosphate pathway/shunt, an alternative to glycolysis and a major source of reducing power and metabolic intermediates for biosynthetic processes (By similarity). Has a hexose-6-phosphate dehydrogenase activity, with broad substrate specificity compared to glucose-6-phosphate 1-dehydrogenase/G6PD, and catalyzes the first step of the pentose phosphate pathway (PubMed : 12858176, PubMed : 18628520, PubMed : 23132696). In addition, acts as a 6-phosphogluconolactonase and catalyzes the second step of the pentose phosphate pathway (By similarity). May have a dehydrogenase activity for alternative substrates including glucosamine 6-phosphate and glucose 6-sulfate (By similarity). The main function of this enzyme is to provide reducing equivalents such as NADPH to maintain the adequate levels of reductive cofactors in the oxidizing environment of the endoplasmic reticulum (PubMed : 12858176, PubMed : 18628520, PubMed : 23132696). By producing NADPH that is needed by reductases of the lumen of the endoplasmic reticulum like corticosteroid 11-beta-dehydrogenase isozyme 1/HSD11B1, indirectly regulates their activity (PubMed : 18628520).
See full target information H6PD
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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

NPJ precision oncology 9:213 PubMed40595024

2025

Deciphering the YY1/ lncRNA BLACAT1/miR-605-3p axis in glioblastoma: implications for therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Han,Han Wang,Hong Xie,Xiuming Yu,Haixia Zhou,Zhigang Guo

Acta biochimica et biophysica Sinica 57:1670-1683 PubMed40264416

2025

Single-cell transcriptomic data reveal the cellular heterogeneity of glutamine metabolism in gastric premalignant lesions and early gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Qingfeng Ni,Jiawei Yu,Yuanjie Niu,Zhenwei Han,Boyang Hu,Yang Wang,Jianwei Zhu

Antioxidants (Basel, Switzerland) 13: PubMed39594539

2024

Redox Imbalance and Antioxidant Defenses Dysfunction: Key Contributors to Early Aging in Childhood Cancer Survivors.

Applications

Unspecified application

Species

Unspecified reactive species

Vanessa Cossu,Nadia Bertola,Chiara Fresia,Federica Sabatini,Silvia Ravera

Heliyon 10:e38718 PubMed39403483

2024

Mandatory role of endoplasmic reticulum in preserving NADPH regeneration in starved MDA-MB-231 breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sonia Carta,Vanessa Cossu,Francesca Vitale,Matteo Bauckneht,Maddalena Ghelardoni,Anna Maria Orengo,Serena Losacco,Daniela Gaglio,Silvia Bruno,Sabrina Chiesa,Silvia Ravera,Gianmario Sambuceti,Cecilia Marini

Molecular and cellular biochemistry 479:2973-2987 PubMed38082185

2023

Mandatory role of endoplasmic reticulum and its pentose phosphate shunt in the myocardial defense mechanisms against the redox stress induced by anthracyclines.

Applications

Unspecified application

Species

Unspecified reactive species

Gianmario Sambuceti,Vanessa Cossu,Francesca Vitale,Eva Bianconi,Sonia Carta,Consuelo Venturi,Sabrina Chiesa,Francesco Lanfranchi,Laura Emionite,Sebastiano Carlone,Luca Sofia,Francesca D'Amico,Tania Di Raimondo,Silvia Chiola,Anna Maria Orengo,Silvia Morbelli,Pietro Ameri,Matteo Bauckneht,Cecilia Marini

iScience 26:108137 PubMed37867937

2023

A fingerprint of 2-[F]FDG radiometabolites - How tissue-specific metabolism beyond 2-[F]FDG-6-P could affect tracer accumulation.

Applications

Unspecified application

Species

Unspecified reactive species

Eva-Maria Patronas,Theresa Balber,Anne Miller,Barbara Katharina Geist,Antje Michligk,Chrysoula Vraka,Maximilian Krisch,Nataliya Rohr-Udilova,Arvand Haschemi,Helmut Viernstein,Marcus Hacker,Markus Mitterhauser

Cell metabolism 35:1261-1279.e11 PubMed37141889

2023

Organism-wide, cell-type-specific secretome mapping of exercise training in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Wei,Nicholas M Riley,Xuchao Lyu,Xiaotao Shen,Jing Guo,Steffen H Raun,Meng Zhao,Maria Dolores Moya-Garzon,Himanish Basu,Alan Sheng-Hwa Tung,Veronica L Li,Wentao Huang,Amanda L Wiggenhorn,Katrin J Svensson,Michael P Snyder,Carolyn R Bertozzi,Jonathan Z Long

Antioxidants (Basel, Switzerland) 12: PubMed36670904

2022

Fundamental Role of Pentose Phosphate Pathway within the Endoplasmic Reticulum in Glutamine Addiction of Triple-Negative Breast Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Cecilia Marini,Vanessa Cossu,Sonia Carta,Elisa Greotti,Daniela Gaglio,Nadia Bertola,Sabrina Chiesa,Silvia Bruno,Francesca Vitale,Marcella Bonanomi,Danilo Porro,Mattia Riondato,Anna Maria Orengo,Matteo Bauckneht,Silvia Morbelli,Silvia Ravera,Gianmario Sambuceti

Cells 11: PubMed35954197

2022

Mutated Gene Role in the Modulation of Energy Metabolism and Mitochondrial Dynamics in Head and Neck Squamous Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Nadia Bertola,Paolo Degan,Enrico Cappelli,Silvia Ravera

Journal of translational medicine 20:120 PubMed35272674

2022

RUNX3-mediated circDYRK1A inhibits glutamine metabolism in gastric cancer by up-regulating microRNA-889-3p-dependent FBXO4.

Applications

Unspecified application

Species

Unspecified reactive species

Haofeng Liu,Qiu Xue,Hongzhou Cai,Xiaohui Jiang,Guangxin Cao,Tie Chen,Yuan Chen,Ding Wang
View all publications
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