Anti-HA tag antibody [12CA5]

• WB

AbReview31775****

Western blot - Anti-HA tag antibody [12CA5] (AB1424)

Blocked with 5% milk for 30 min at 25°C

All lanes:

Western blot - Anti-HA tag antibody [12CA5] (ab1424) at 1/1000 dilution

All lanes:

HEK293T whole cell lysate over-expressing HA-tagged Rab6 at 20 µg

Secondary

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HRP-conjugated goat anti-mouse polyclonal IgG at 1/5000 dilution

Predicted band size: 64 kDa

true

Exposure time: 5s

This image is courtesy of an anonymous Abreview

• IP

AbReview34087****

Immunoprecipitation - Anti-HA tag antibody [12CA5] (AB1424)

Recombinant HA-tagged Rab6 was immunoprecipitated from 1 mg transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with 2 μL ab1424.

Lane 1 : Rab6-HA transfected HEK-293T whole cell extract (Input)

Lane 2 : ab1424 IP in Rab6-HA transfected HEK-293T whole cell extract.

All lanes:

Immunoprecipitation - Anti-HA tag antibody [12CA5] (ab1424)

Predicted band size: 64 kDa

true

Data courtesy of an anonymous AbReview

• ICC/IF

AbReview2795****

Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [12CA5] (AB1424)

This picture shows baby hamster kidney cell expressing HA-tag CFTR protein. This type of cells were grown on coverslip and immunofluorescence was performed. Dilution of 1 : 200 was used and incubated for 2hrs with antibody.

This image was kindly supplied as part of the review submitted by one of our customers.

• ChIP

Unknown

ChIP - Anti-HA tag antibody [12CA5] (AB1424)

Xenopus laevis oocytes were injected with mRNA for HA-tagged human BORIS. Chromatin was prepared according to the Abcam X-ChIP protocol. Oocytes were fixed with formaldehyde for 10min. The ChIP was performed with 25μg of chromatin, 3μg of ab1424 (anti-HA, light blue) and 3μg of ab18337 (anti-BORIS, dark blue), and 20μl of Protein A/G sepharose beads. A non-specific antibody was used as a control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach).