Anti-HA tag antibody [12CA5] is a mouse monoclonal antibody that is provided in a PBS only formulation free from BSA and azide. Formulated to be conjugation-ready. It is used to detect HA tag in ChIP, ICC/IF, IP, Western blot.
- Trusted by researchers since 2002
- Cited in over 70 publications
Images
![Western blot - Anti-HA tag antibody [12CA5] (AB1424), expandable thumbnail](https://content.abcam.com/products/images/ha-tag-antibody-12ca5-ab1424--western-blot-img137353.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-HA tag antibody [12CA5] (AB1424)")
![Immunoprecipitation - Anti-HA tag antibody [12CA5] (AB1424), expandable thumbnail](https://content.abcam.com/products/images/ha-tag-antibody-12ca5-ab1424--immunoprecipitation-img82.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-HA tag antibody [12CA5] (AB1424)")
![Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [12CA5] (AB1424), expandable thumbnail](https://content.abcam.com/products/images/ha-tag-antibody-12ca5-ab1424--immunocytochemistry-immunofluorescence-img114349.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [12CA5] (AB1424)")
![ChIP - Anti-HA tag antibody [12CA5] (AB1424), expandable thumbnail](https://content.abcam.com/products/images/ha-tag-antibody-12ca5-ab1424--chip-img21068.jpg/_jcr_content/renditions/webp-475.webp "ChIP - Anti-HA tag antibody [12CA5] (AB1424)")
Publications
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- Molecular neurobiology 61:1726-17362023Deubiquitylating Enzyme OTUB1 Facilitates Neuronal Survival After Intracerebral Hemorrhage Via Inhibiting NF-κB-triggered Apoptotic Cascades.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiabing Shen,Xiaoli Xue,Huimin Yuan,Yan Song,Jinglei Wang,Ronghui Cui,Kaifu KePubMed 37775718
- Cell death & disease 14:5182023LMO2 promotes the development of AML through interaction with transcription co-regulator LDB1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLihui Lu,Jianwei Wang,Fang Fang,Ailian Guo,Shuting Jiang,Yanfang Tao,Yongping Zhang,Yan Li,Kunlong Zhang,Zimu Zhang,Ran Zhuo,Xinran Chu,Xiaolu Li,Yuanyuan Tian,Li Ma,Xu Sang,Yanling Chen,Juanjuan Yu,Yang Yang,Haibo Cao,Jizhao Gao,Jun Lu,Shaoyan Hu,Jian Pan,Hailong HePubMed 37573405
- Cellular oncology (Dordrecht) 46:1493-15072023TRIM17-mediated ubiquitination and degradation of RBM38 promotes cisplatin resistance in non-small cell lung cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTian Zhong,Jing Zhang,Xingren Liu,Hongmin LiPubMed 37219768
- International journal of molecular sciences 24:2023The Histone Deacetylases Hst1 and Rpd3 Integrate De Novo NAD Metabolism with Phosphate Sensing in .Applications:Unspecified applicationReactive species:Unspecified reactive speciesBenjamin Groth,Yi-Ching Lee,Chi-Chun Huang,Matilda McDaniel,Katie Huang,Lan-Hsuan Lee,Su-Ju LinPubMed 37175754
- Nature 616:168-1752023RHOJ controls EMT-associated resistance to chemotherapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaud Debaugnies,Sara Rodríguez-Acebes,Jeremy Blondeau,Marie-Astrid Parent,Manuel Zocco,Yura Song,Viviane de Maertelaer,Virginie Moers,Mathilde Latil,Christine Dubois,Katia Coulonval,Francis Impens,Delphi Van Haver,Sara Dufour,Akiyoshi Uemura,Panagiota A Sotiropoulou,Juan Méndez,Cédric BlanpainPubMed 36949199
- Frontiers in pharmacology 14:11386582023Platycodin-D exerts its anti-cancer effect by promoting c-Myc protein ubiquitination and degradation in gastric cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQianqian Xu,Guangzhao Pan,Zhonglan Wang,Lingling Wang,Yancheng Tang,Jinyun Dong,Jiang-Jiang QinPubMed 36950011
- Journal of experimental & clinical cancer research : CR 42:52023KLF14 regulates the growth of hepatocellular carcinoma cells via its modulation of iron homeostasis through the repression of iron-responsive element-binding protein 2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHui Zhou,Junru Chen,Mingjie Fan,Huajian Cai,Yufei Dong,Yue Qiu,Qianqian Zhuang,Zhaoying Lei,Mengyao Li,Xue Ding,Peng Yan,Aifu Lin,Shusen Zheng,Qingfeng YanPubMed 36600258
- iScience 26:1057452023Centrosomal protein 120 promotes centrosome amplification and gastric cancer progression via USP54-mediated deubiquitination of PLK4.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChenggang Zhang,Xianxiong Ma,Guanxin Wei,Xiuxian Zhu,Peng Hu,Xiang Chen,Dianshi Wang,Yuan Li,Tuo Ruan,Weikang Zhang,Kaixiong Tao,Chuanqing WuPubMed 36590171
- International journal of molecular sciences 23:2022Extends Life Span and Promotes Fitness and Stress Resistance in .Applications:Unspecified applicationReactive species:Unspecified reactive speciesRubing Xue,Ke Yang,Fuhui Xiao,Liping Yang,Guijun Chen,Yongxuan Li,Yunshuang Ye,Kangning Chen,Sheryl T Smith,Gonghua Li,Qingpeng Kong,Jumin ZhouPubMed 36430913
- Cell death & disease 13:7902022Phosphorylation of PFKFB4 by PIM2 promotes anaerobic glycolysis and cell proliferation in endometriosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChao Lu,Pengyun Qiao,Ruihai Fu,Yadi Wang,Jiayi Lu,Xi Ling,Lu Liu,Yujun Sun,Chune Ren,Zhenhai YuPubMed 36109523
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Storage buffer
Constituents: 1% Dibasic monohydrogen potassium phosphate
- Form
- Liquid
- Clonality
- Monoclonal
Reactivity data
Select an application| IP | ChIP | WB | ICC/IF | |
|---|---|---|---|---|
| Tag | Tested | Tested | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info 10 µg/mL | Notes - |
ChIP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info 3 µg/mL | Notes Use 3 μg per 25 μg chromatin (see figure legend). |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info 1 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info 10 µg/mL | Notes - |
Associated Products
Select an associated product type
8 products for Alternative Product
Target data
Alternative names
HA epitope tag, HA2, Hemagglutinin, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain
Recommended products
Anti-HA tag antibody [12CA5] is a mouse monoclonal antibody that is provided in a PBS only formulation free from BSA and azide. Formulated to be conjugation-ready. It is used to detect HA tag in ChIP, ICC/IF, IP, Western blot.
- Trusted by researchers since 2002
- Cited in over 70 publications
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Storage buffer
Constituents: 1% Dibasic monohydrogen potassium phosphate
- Form
- Liquid
- Clonality
- Monoclonal
- Carrier free
- Yes
- Clone number
- 12CA5
- Purification technique
- Affinity purification
- Specificity
The antibody detects HA-tag fused to the C-terminus or N-terminus of targeted proteins in transfected cells.The antibody works in ChIP but less well than ab9110 (see ChIP data).
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-HA tag antibody [12CA5] (ab1424) is a mouse monoclonal antibody provided in a carrier-free, conjugation ready format.
ab1424 is validated in ChIP, ICC/IF, IP and WB.
Carrier-free antibodies are compatible with our best-in-class range of Lightning-Link® conjugation kits.
Anti-HA tag antibody [12CA5] (ab1424) specifically detects HA tag and is sold in 200 µg selling sizes.
Quality and Validation
Antibody clone 12CA5 has been cited over 75 times in peer reviewed journals and is trusted by the scientific community.
Anti-HA tag antibody [12CA5] (ab1424) has 12 independent reviews from customers.
This product is manufactured by BioVision, an Abcam company and was previously called 3996 HA-Tag Antibody (Clone 12CA5[2]).
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-HA tag antibody [12CA5] (ab1424), expandable thumbnail](https://content.abcam.com/products/images/ha-tag-antibody-12ca5-ab1424--western-blot-img137353.jpg "Western blot - Anti-HA tag antibody [12CA5] (ab1424)")
This image is courtesy of an anonymous customer reviewWestern blot - Anti-HA tag antibody [12CA5] (ab1424)
Blocked with 5% milk for 30 min at 25°C
All lanes: Western blot - Anti-HA tag antibody [12CA5] (ab1424) at 1/1000 dilution
All lanes: HEK293T whole cell lysate over-expressing HA-tagged Rab6 at 20 µg
SecondaryAll lanes: HRP-conjugated goat anti-mouse polyclonal IgG at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 64 kDa
Exposure time: 5s
![Immunoprecipitation - Anti-HA tag antibody [12CA5] (ab1424), expandable thumbnail](https://content.abcam.com/products/images/ha-tag-antibody-12ca5-ab1424--immunoprecipitation-img82.jpg "Immunoprecipitation - Anti-HA tag antibody [12CA5] (ab1424)")
Data courtesy of an anonymous customer reviewImmunoprecipitation - Anti-HA tag antibody [12CA5] (ab1424)
Recombinant HA-tagged Rab6 was immunoprecipitated from 1 mg transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with 2 μL ab1424.
Lane 1: Rab6-HA transfected HEK-293T whole cell extract (Input)
Lane 2: ab1424 IP in Rab6-HA transfected HEK-293T whole cell extract.
All lanes: Immunoprecipitation - Anti-HA tag antibody [12CA5] (ab1424)
Developed using the ECL technique.
Predicted band size: 64 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [12CA5] (ab1424), expandable thumbnail](https://content.abcam.com/products/images/ha-tag-antibody-12ca5-ab1424--immunocytochemistry-immunofluorescence-img114349.jpg "Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [12CA5] (ab1424)")
This image was kindly supplied as part of the review submitted by one of our customers.Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [12CA5] (ab1424)
This picture shows baby hamster kidney cell expressing HA-tag CFTR protein. This type of cells were grown on coverslip and immunofluorescence was performed. Dilution of 1:200 was used and incubated for 2hrs with antibody.
![ChIP - Anti-HA tag antibody [12CA5] (ab1424), expandable thumbnail](https://content.abcam.com/products/images/ha-tag-antibody-12ca5-ab1424--chip-img21068.jpg "ChIP - Anti-HA tag antibody [12CA5] (ab1424)")
ChIP - Anti-HA tag antibody [12CA5] (ab1424)
Xenopus laevis oocytes were injected with mRNA for HA-tagged human BORIS. Chromatin was prepared according to the Abcam X-ChIP protocol. Oocytes were fixed with formaldehyde for 10min. The ChIP was performed with 25μg of chromatin, 3μg of ab1424 (anti-HA, light blue) and 3μg of ab18337 (anti-BORIS, dark blue), and 20μl of Protein A/G sepharose beads. A non-specific antibody was used as a control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach).
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