Anti-HA tag antibody

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody (AB137838)

Immunofluorescence analysis of transfected paraformaldehyde-fixed HeLa cells, labelling HA-recombinant protein using ab137838 antibody at 1/500 dilution. Right panel costained with Hoechst 33342.

• IP

Supplier Data

Immunoprecipitation - Anti-HA tag antibody (AB137838)

Immunoprecipitation of HA tag protein from 293T whole cell extracts using 5 μg of ab137838. Western blot analysis was performed using HA tag antibody diluted at 1 : 2500. A HRP-conjugated anti rabbit IgG antibody) was used to detect the primary antibody.

All lanes:

Immunoprecipitation - Anti-HA tag antibody (ab137838) at 1/2500 dilution

Lane 1:

HA tag protein from 293T whole cell extracts (Input)

Lane 2:

Control IgG (-)

Lane 3:

HA tag protein from 293T whole cell extracts (+)

Secondary

All lanes:

HRP-conjugated anti rabbit IgG antibody

false

• WB

Supplier Data

Western blot - Anti-HA tag antibody (AB137838)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-HA tag antibody (ab137838) at 1/3000 dilution

Lane 1:

Non-transfected (-) 293T whole cell extracts at 30 µg

Lane 2:

Transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

• ELISA

Supplier Data

ELISA - Anti-HA tag antibody (AB137838)

An ELISA plate is coated with 50 μL of a HA-Tag conjugated BSA at concentration of 5 μg/mL. The peptide conjugated carrier protein is coated and detected with ab137838 at concentration ranged from 3.1 to 200 ng/mL.