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AB13834

Anti-HA tag antibody

5

(3 Reviews)

|

(15 Publications)

Rabbit Polyclonal HA tag antibody. Suitable for WB and reacts with Tag samples. Cited in 15 publications.
1 Images
Western blot - Anti-HA tag antibody (AB13834)
  • WB

Supplier Data

Western blot - Anti-HA tag antibody (AB13834)

Western blot using ab13834 on 293 cells transfected with a 55 kDa HA-tagged minigene (ab20896). Lane 1 : ab13834 Lane 2 : ab13834 with blocking peptide ab13835. Primary : Rabbit polyclonal to HA tag (1/500) Secondary : Alexa Fluor 680 Goat anti Rabbit IgG (1/5000). Lysates at 10µg/lane.

All lanes:

Western blot - Anti-HA tag antibody (ab13834) at 1/500 dilution

Lane 1:

HEK-293 (Human) Whole Cell Lysate - expressing HA tag protein (<a href='/en-us/products/unavailable/hek-293-human-whole-cell-lysate-expressing-ha-tag-protein-ab20896'>ab20896</a>) at 10 µg

Lane 2:

HEK-293 (Human) Whole Cell Lysate - expressing HA tag protein (<a href='/en-us/products/unavailable/hek-293-human-whole-cell-lysate-expressing-ha-tag-protein-ab20896'>ab20896</a>) at 10 µg with HA tag peptide (<a href='/en-us/products/proteins-peptides/ha-tag-peptide-ab13835'>ab13835</a>)

Secondary

All lanes:

Alexa Fluor 680 Goat-anti-Rabbit IgG at 1/5000 dilution

Predicted band size: 64 kDa

Observed band size: 55 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Publications (15)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed37047592

2023

NF-κB Transcriptional Activity Indispensably Mediates Hypoxia-Reoxygenation Stress-Induced microRNA-210 Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Gurdeep Marwarha,Katrine Hordnes Slagsvold,Morten Andre Høydal

Microbiology spectrum 10:e0150922 PubMed36326498

2022

Ubiquitination of SARS-CoV-2 ORF7a Prevents Cell Death Induced by Recruiting BclXL To Activate ER Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Zhixin Liu,Yanan Fu,Yanping Huang,Feng Zeng,Jingjing Rao,Xiao Xiao,Xiaoguang Sun,Hao Jin,Jian Li,Jing Yang,Weixing Du,Long Liu

The Plant journal : for cell and molecular biology 112:966-981 PubMed36168109

2022

OsDWD1 E3 ligase-mediated OsNPR1 degradation suppresses basal defense in rice.

Applications

Unspecified application

Species

Unspecified reactive species

Changhyun Choi,Jong Hee Im,Jinjeong Lee,Soon Il Kwon,Woe-Yeon Kim,Sang Ryeol Park,Duk-Ju Hwang

International journal of molecular sciences 23: PubMed36012628

2022

GSK3β Inhibition Is the Molecular Pivot That Underlies the Mir-210-Induced Attenuation of Intrinsic Apoptosis Cascade during Hypoxia.

Applications

Unspecified application

Species

Unspecified reactive species

Gurdeep Marwarha,Øystein Røsand,Katrine Hordnes Slagsvold,Morten Andre Høydal

Journal of integrative plant biology 64:1706-1723 PubMed35848532

2022

SICKLE represses photomorphogenic development of Arabidopsis seedlings via HY5- and PIF4-mediated signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Li,Haojie Li,Hongmei Lian,Pengyu Song,Yulong Wang,Jie Duan,Zhaoqing Song,Yan Cao,Dongqing Xu,Jigang Li,Huiyong Zhang

Nature communications 12:6412 PubMed34741010

2021

Crosstalk between CST and RPA regulates RAD51 activity during replication stress.

Applications

Unspecified application

Species

Unspecified reactive species

Kai-Hang Lei,Han-Lin Yang,Hao-Yen Chang,Hsin-Yi Yeh,Dinh Duc Nguyen,Tzu-Yu Lee,Xinxing Lyu,Megan Chastain,Weihang Chai,Hung-Wen Li,Peter Chi

iScience 24:103228 PubMed34746697

2021

MYB70 modulates seed germination and root system development in .

Applications

Unspecified application

Species

Unspecified reactive species

Jinpeng Wan,Ruling Wang,Ping Zhang,Liangliang Sun,Qiong Ju,Haodong Huang,Shiyou Lü,Lam-Son Tran,Jin Xu

The Journal of biological chemistry 294:9873-9887 PubMed31076507

2019

Heat shock protein DNAJA1 stabilizes PIWI proteins to support regeneration and homeostasis of planarian .

Applications

Unspecified application

Species

Unspecified reactive species

Chen Wang,Zhen-Zhen Yang,Fang-Hao Guo,Shuo Shi,Xiao-Shuai Han,An Zeng,Haifan Lin,Qing Jing

Cell cycle (Georgetown, Tex.) 15:1961-74 PubMed27152953

2016

Proteomic analysis of Rac1 signaling regulation by guanine nucleotide exchange factors.

Applications

Unspecified application

Species

Unspecified reactive species

Hadir Marei,Alejandro Carpy,Boris Macek,Angeliki Malliri

eLife 5:e10647 PubMed26949256

2016

SOX2 O-GlcNAcylation alters its protein-protein interactions and genomic occupancy to modulate gene expression in pluripotent cells.

Applications

Unspecified application

Species

Unspecified reactive species

Samuel A Myers,Sailaja Peddada,Nilanjana Chatterjee,Tara Friedrich,Kiichrio Tomoda,Gregor Krings,Sean Thomas,Jason Maynard,Michael Broeker,Matthew Thomson,Katherine Pollard,Shinya Yamanaka,Alma L Burlingame,Barbara Panning
View all publications

Product promise

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