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Rabbit Polyclonal HA tag antibody. Suitable for ELISA, WB, ICC/IF and reacts with Tag samples. Cited in 17 publications.


Images

Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody (AB20084), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Preservative: 0.1% Sodium azide
Constituents: PBS

Form

Liquid

Clonality

Polyclonal

Consider this alternative

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ELISAWBICC/IF
Tag
Expected
Expected
Tested

Expected
Expected

Species

Tag

Dilution info

-

Notes

The anti HA diluted 1/70,000 gave an OD=1.0 in a 15 minute reaction against peptide conjugated with a different carrier than used for anti peptide purification.

Expected
Expected

Species

Tag

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Tag

Dilution info

-

Notes

-

Associated Products

Select an associated product type

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Alternative names

Recommended products

Rabbit Polyclonal HA tag antibody. Suitable for ELISA, WB, ICC/IF and reacts with Tag samples. Cited in 17 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Purification technique

Affinity purification Immunogen

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

The antibody ab20084 is able to recognise the HA epitope when used to tag other proteins.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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1 product image

  • Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody (ab20084), expandable thumbnail
    Image from Wu CY et al., PLoS One. 2010 Mar 22;5(3):e9784. doi: 10.1371/journal.pone.0009784.; Fig 1.; March 22, 2010, PLoS ONE 5(3): e9784.

    Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody (ab20084)

    Immunofluorescence analysis of influenza virus-like particle producer cells, staining Hemagglutinin (red) with ab20084.


    Cells were fixed in paraformaldehyde for 10 min and permeabilized in 0.05% Triton-X 100 for 1 min. After blocking with 1% gelatin, the cells were incubated with primary antibody, followed by incubation with Cy3-conjugated goat anti-rabbit IgG as the secondary antibody.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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