AB20084

Anti-HA tag antibody

Lab Essentials
What is this?

4

(2 Reviews)

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(21 Publications)

Rabbit Polyclonal HA tag antibody. Suitable for ELISA, WB, ICC/IF and reacts with Tag samples. Cited in 21 publications.

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody (AB20084)

ICC/IF

PubMed

Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody (AB20084)

Immunofluorescence analysis of influenza virus-like particle producer cells, staining Hemagglutinin (red) with ab20084.

Cells were fixed in paraformaldehyde for 10 min and permeabilized in 0.05% Triton-X 100 for 1 min. After blocking with 1% gelatin, the cells were incubated with primary antibody, followed by incubation with Cy3-conjugated goat anti-rabbit IgG as the secondary antibody.

Image from Wu CY et al., PLoS One. 2010 Mar 22;5(3):e9784. doi: 10.1371/journal.pone.0009784.; Fig 1.; March 22, 2010, PLoS ONE 5(3): e9784.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Applications

WB, ICC/IF, ELISA

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p>The anti HA diluted 1/70,000 gave an OD=1.0 in a 15 minute reaction against peptide conjugated with a different carrier than used for anti peptide purification.</p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }

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Product details

The antibody ab20084 is able to recognise the HA epitope when used to tag other proteins.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.1% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information HA tag

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Publications (21)

Recent publications for all applications. Explore the full list and refine your search

EMBO reports 25:3486-3505 PubMed38890452

2024

PHF2-mediated H3K9me balance orchestrates heterochromatin stability and neural progenitor proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Samuel Aguirre,Stella Pappa,Núria Serna-Pujol,Natalia Padilla,Simona Iacobucci,A Silvina Nacht,Guillermo P Vicent,Albert Jordan,Xavier de la Cruz,Marian A Martínez-Balbás

PLoS pathogens 20:e1012034 PubMed38814986

2024

A novel ilarvirus protein CP-RT is expressed via stop codon readthrough and suppresses RDR6-dependent RNA silencing.

Applications

Unspecified application

Species

Unspecified reactive species

Nina Lukhovitskaya,Katherine Brown,Lei Hua,Adrienne E Pate,John P Carr,Andrew E Firth

iScience 26:108080 PubMed37860693

2023

The SARS-CoV-2 protein ORF3c is a mitochondrial modulator of innate immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Hazel Stewart,Yongxu Lu,Sarah O'Keefe,Anusha Valpadashi,Luis Daniel Cruz-Zaragoza,Hendrik A Michel,Samantha K Nguyen,George W Carnell,Nina Lukhovitskaya,Rachel Milligan,Yasmin Adewusi,Irwin Jungreis,Valeria Lulla,David A Matthews,Stephen High,Peter Rehling,Edward Emmott,Jonathan L Heeney,Andrew D Davidson,James R Edgar,Geoffrey L Smith,Andrew E Firth

Nature communications 13:3263 PubMed35672304

2022

JMJD3 intrinsically disordered region links the 3D-genome structure to TGFβ-dependent transcription activation.

Applications

Unspecified application

Species

Unspecified reactive species

Marta Vicioso-Mantis,Raquel Fueyo,Claudia Navarro,Sara Cruz-Molina,Wilfred F J van Ijcken,Elena Rebollo,Álvaro Rada-Iglesias,Marian A Martínez-Balbás

Cell reports 36:109636 PubMed34469722

2021

Patient-specific iPSCs carrying an SFTPC mutation reveal the intrinsic alveolar epithelial dysfunction at the inception of interstitial lung disease.

Applications

Unspecified application

Species

Unspecified reactive species

Konstantinos-Dionysios Alysandratos,Scott J Russo,Anton Petcherski,Evan P Taddeo,Rebeca Acín-Pérez,Carlos Villacorta-Martin,J C Jean,Surafel Mulugeta,Luis R Rodriguez,Benjamin C Blum,Ryan M Hekman,Olivia T Hix,Kasey Minakin,Marall Vedaie,Seunghyi Kook,Andrew M Tilston-Lunel,Xaralabos Varelas,Jennifer A Wambach,F Sessions Cole,Aaron Hamvas,Lisa R Young,Marc Liesa,Andrew Emili,Susan H Guttentag,Orian S Shirihai,Michael F Beers,Darrell N Kotton

Nature communications 11:4070 PubMed32792502

2020

A hidden gene in astroviruses encodes a viroporin.

Applications

Unspecified application

Species

Unspecified reactive species

Valeria Lulla,Andrew E Firth

Cell reports 31:107685 PubMed32460012

2020

TGF-β-Induced Phosphorylation of Usp9X Stabilizes Ankyrin-G and Regulates Dendritic Spine Development and Maintenance.

Applications

Unspecified application

Species

Unspecified reactive species

Sehyoun Yoon,Euan Parnell,Peter Penzes

ACS pharmacology & translational science 3:296-304 PubMed32296769

2020

Distinct Patterns of Internalization of Different Calcitonin Gene-Related Peptide Receptors.

Applications

Unspecified application

Species

Unspecified reactive species

Joseph J Gingell,Tayla A Rees,Erica R Hendrikse,Andrew Siow,David Rennison,John Scotter,Paul W R Harris,Margaret A Brimble,Christopher S Walker,Debbie L Hay

Pharmaceutical biology 57:701-709 PubMed31613681

2019

K-Ras-ERK1/2 accelerates lung cancer cell development via mediating H3 through the MDM2-GCN5-SIRT7 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Ziming Cheng,Xiufeng Li,Shizhen Hou,Yubing Wu,Yi Sun,Bing Liu

Neuron 101:1150-1165.e8 PubMed30713030

2019

Vasoactive Intestinal Polypeptide-Expressing Interneurons in the Hippocampus Support Goal-Oriented Spatial Learning.

Applications

Unspecified application

Species

Unspecified reactive species

Gergely Farkas Turi,Wen-Ke Li,Spyridon Chavlis,Ioanna Pandi,Justin O'Hare,James Benjamin Priestley,Andres Daniel Grosmark,Zhenrui Liao,Max Ladow,Jeff Fang Zhang,Boris Valery Zemelman,Panayiota Poirazi,Attila Losonczy

View all publications

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Product promise

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