AB9111

Anti-HA tag antibody

Name: Anti-HA tag antibody (ab9111) | Abcam
Brand: Abcam Limited
SKU: AB9111
Price: 595 USD
Availability: InStock
Rating: 2 (2 reviews)

(2 Reviews)

(21 Publications)

Anti-HA tag antibody (ab9111) is a chicken polyclonal antibody detecting HA tag in Western Blot, ICC/IF, ELISA.

- Over 20 publications
- Trusted since 2002

View Alternative Names

HA epitope tag, HA2, Hemagglutinin, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody (AB9111)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody (AB9111)

Immunofluorescent analysis of 4% paraformaldehyde (PFA) - fixed, permeabilized with 0.1% Triton X-100 CHO cells transfected with GFP-HA constructs (CHO-GFP-HA) labelling HA tag with ab9111 at 5μg/mL, followed by Goat Anti- Chicken IgY (H&L), Alexa594 conjugated antibody at 2.5 μg/mL (Red). Nucleus was counterstained with DAPI (Blue).

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody (AB9111)

Immunofluorescent analysis of 4% paraformaldehyde (PFA)-fixed, permeabilised with 0.1% Triton X-100 in cells transfected with GFP-HA contrsucts (as CHO-GFP-HA) or parental non- transfected CHO cells labelling with chicken anti-HA tag (ab9111) at 5μg/ml, followed by Goat Anti-Chicken IgY (H&L), Alexa594 conjugated antibody at 2.5μg/ml (Red). Nucleus was counterstained with DAPI (Blue).

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody (AB9111)

Immunofluorescent analysis of 4% paraformaldehyde (PFA) -fixed, permeabilized with 0.1% Triton X-100 CHO cells transfected with 12 tags constructs (CHO-12 tags) labelling HA tag with ab9111 at 1/250 dilution (4μg/mL), followed by Goat Anti-Chicken IgY (H&L), Alexa594 conjugated antibody at 1/500 dilution (2 μg/mL) (Red). Nucleus was counterstained with DAPI (white). Bottom panel images showed Parallel staining in parental CHO cell line.

Key facts

Host species

Chicken

Clonality

Polyclonal

Isotype

Unknown

Light chain type

unknown

Carrier free

Applications

WB, ICC/IF, ELISA

applications

Specificity

ELISA: this antibody diluted at 1:20,000 gave an O.D.= 1.0 in a 15 minute reaction against peptide conjugated to a different carrier than used for anti peptide purification. HRP conjugated goat anti chicken IgG (IgY) was used and TMB was the substrate.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "4-5 µg/mL", "ICCIF-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-HA tag antibody (ab9111) is a chicken polyclonal antibody and is validated for use in Western Blot (WB), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA. Tagged protein detection
HA tag antibodies are used to visualize proteins labelled with this tag in a variety of applications.

Trusted by the scientific community
Anti-HA tag (ab9111) was first used in a scientific publication in 2002 and has been cited over 20 times in peer-reviewed journals.

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Properties and storage information

Form

Liquid

Purity

IgY fraction

Purification notes

Antibodies were immunoaffinity purified using the peptide immobilized on a solid support.

Storage buffer

pH: 7.2 Preservative: 0.1% Sodium azide

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information HA tag

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Publications (21)

Recent publications for all applications. Explore the full list and refine your search

Acta neuropathologica communications 11:200 PubMed38111057

2023

Targeting RACK1 to alleviate TDP-43 and FUS proteinopathy-mediated suppression of protein translation and neurodegeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Beibei Zhao,Catherine M Cowan,Juliane A Coutts,Darren D Christy,Ananya Saraph,Shawn C C Hsueh,Stephen S Plotkin,Ian R Mackenzie,Johanne M Kaplan,Neil R Cashman

Nature communications 14:6492 PubMed37838698

2023

Mitigating a TDP-43 proteinopathy by targeting ataxin-2 using RNA-targeting CRISPR effector proteins.

Applications

Unspecified application

Species

Unspecified reactive species

M Alejandra Zeballos C,Hayden J Moore,Tyler J Smith,Jackson E Powell,Najah S Ahsan,Sijia Zhang,Thomas Gaj

Journal of virology 96:e0090022 PubMed36040180

2022

Evidence for Viral mRNA Export from Ebola Virus Inclusion Bodies by the Nuclear RNA Export Factor NXF1.

Applications

Unspecified application

Species

Unspecified reactive species

Lisa Wendt,Janine Brandt,Dmitry S Ushakov,Bianca S Bodmer,Matthew J Pickin,Allison Groseth,Thomas Hoenen

Science advances 8:eabk2485 PubMed35044815

2022

Targeted gene silencing in the nervous system with CRISPR-Cas13.

Applications

Unspecified application

Species

Unspecified reactive species

Jackson E Powell,Colin K W Lim,Ramya Krishnan,Tristan X McCallister,Christian Saporito-Magriña,Maria A Zeballos,Garrett D McPheron,Thomas Gaj

Epigenetics & chromatin 14:35 PubMed34321103

2021

Epigenetic rewriting at centromeric DNA repeats leads to increased chromatin accessibility and chromosomal instability.

Applications

Unspecified application

Species

Unspecified reactive species

Sheldon Decombe,François Loll,Laura Caccianini,Kévin Affannoukoué,Ignacio Izeddin,Julien Mozziconacci,Christophe Escudé,Judith Lopes

PLoS biology 19:e3001323 PubMed34228711

2021

Synaptotagmin-7-mediated activation of spontaneous NMDAR currents is disrupted in bipolar disorder susceptibility variants.

Applications

Unspecified application

Species

Unspecified reactive species

Qiu-Wen Wang,Ying-Han Wang,Bing Wang,Yun Chen,Si-Yao Lu,Jun Yao

Molecular pharmacology 98:677-685 PubMed33008920

2020

N-Terminal Targeting of Regulator of G Protein Signaling Protein 2 for F-Box Only Protein 44-Mediated Proteasomal Degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Harrison J McNabb,Stephanie Gonzalez,Christine S Muli,Benita Sjögren

Molecular therapy : the journal of the American So 28:1177-1189 PubMed31991108

2020

Treatment of a Mouse Model of ALS by In Vivo Base Editing.

Applications

Unspecified application

Species

Unspecified reactive species

Colin K W Lim,Michael Gapinske,Alexandra K Brooks,Wendy S Woods,Jackson E Powell,M Alejandra Zeballos C,Jackson Winter,Pablo Perez-Pinera,Thomas Gaj

Nature communications 10:5490 PubMed31792216

2019

A cancer rainbow mouse for visualizing the functional genomics of oncogenic clonal expansion.

Applications

Unspecified application

Species

Unspecified reactive species

Peter G Boone,Lauren K Rochelle,Joshua D Ginzel,Veronica Lubkov,Wendy L Roberts,P J Nicholls,Cheryl Bock,Mei Lang Flowers,Richard J von Furstenberg,Barry R Stripp,Pankaj Agarwal,Alexander D Borowsky,Robert D Cardiff,Larry S Barak,Marc G Caron,H Kim Lyerly,Joshua C Snyder

eLife 8: PubMed31577228

2019

Matrix metalloproteinase 1 modulates invasive behavior of tracheal branches during entry into flight muscles.

Applications

Unspecified application

Species

Unspecified reactive species

Julia Sauerwald,Wilko Backer,Till Matzat,Frank Schnorrer,Stefan Luschnig

View all publications

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