Chicken Polyclonal HA tag antibody. Suitable for ICC/IF, ELISA, WB and reacts with Tag samples. Cited in 21 publications.
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- Acta neuropathologica communications 11:2002023Targeting RACK1 to alleviate TDP-43 and FUS proteinopathy-mediated suppression of protein translation and neurodegeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBeibei Zhao,Catherine M Cowan,Juliane A Coutts,Darren D Christy,Ananya Saraph,Shawn C C Hsueh,Stephen S Plotkin,Ian R Mackenzie,Johanne M Kaplan,Neil R CashmanPubMed 38111057
- Nature communications 14:64922023Mitigating a TDP-43 proteinopathy by targeting ataxin-2 using RNA-targeting CRISPR effector proteins.Applications:Unspecified applicationReactive species:Unspecified reactive speciesM Alejandra Zeballos C,Hayden J Moore,Tyler J Smith,Jackson E Powell,Najah S Ahsan,Sijia Zhang,Thomas GajPubMed 37838698
- Journal of virology 96:e00900222022Evidence for Viral mRNA Export from Ebola Virus Inclusion Bodies by the Nuclear RNA Export Factor NXF1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLisa Wendt,Janine Brandt,Dmitry S Ushakov,Bianca S Bodmer,Matthew J Pickin,Allison Groseth,Thomas HoenenPubMed 36040180
- Science advances 8:eabk24852022Targeted gene silencing in the nervous system with CRISPR-Cas13.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJackson E Powell,Colin K W Lim,Ramya Krishnan,Tristan X McCallister,Christian Saporito-Magriña,Maria A Zeballos,Garrett D McPheron,Thomas GajPubMed 35044815
- Epigenetics & chromatin 14:352021Epigenetic rewriting at centromeric DNA repeats leads to increased chromatin accessibility and chromosomal instability.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSheldon Decombe,François Loll,Laura Caccianini,Kévin Affannoukoué,Ignacio Izeddin,Julien Mozziconacci,Christophe Escudé,Judith LopesPubMed 34321103
- PLoS biology 19:e30013232021Synaptotagmin-7-mediated activation of spontaneous NMDAR currents is disrupted in bipolar disorder susceptibility variants.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQiu-Wen Wang,Ying-Han Wang,Bing Wang,Yun Chen,Si-Yao Lu,Jun YaoPubMed 34228711
- Molecular pharmacology 98:677-6852020N-Terminal Targeting of Regulator of G Protein Signaling Protein 2 for F-Box Only Protein 44-Mediated Proteasomal Degradation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHarrison J McNabb,Stephanie Gonzalez,Christine S Muli,Benita SjögrenPubMed 33008920
- Molecular therapy : the journal of the American So 28:1177-11892020Treatment of a Mouse Model of ALS by In Vivo Base Editing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesColin K W Lim et. al.PubMed 31991108
- Nature communications 10:54902019A cancer rainbow mouse for visualizing the functional genomics of oncogenic clonal expansion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPeter G Boone et. al.PubMed 31792216
- eLife 8:2019Matrix metalloproteinase 1 modulates invasive behavior of tracheal branches during entry into flight muscles.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJulia Sauerwald et. al.PubMed 31577228
Key facts
- Isotype
- Unknown
- Host species
- Chicken
- Storage buffer
pH: 7.2
Preservative: 0.1% Sodium azide- Form
- Liquid
- Clonality
- Polyclonal
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Reactivity data
Select an application| ICC/IF | ELISA | WB | |
|---|---|---|---|
| Tag | Tested | Expected | Expected |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info 4-5 µg/mL | Notes - |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info Use at an assay dependent concentration. | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
Target data
Alternative names
HA epitope tag, HA2, Hemagglutinin, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain
Recommended products
Chicken Polyclonal HA tag antibody. Suitable for ICC/IF, ELISA, WB and reacts with Tag samples. Cited in 21 publications.
Key facts
- Isotype
- Unknown
- Host species
- Chicken
- Storage buffer
pH: 7.2
Preservative: 0.1% Sodium azide- Form
- Liquid
- Clonality
- Polyclonal
- Purity
- IgY fraction
- Specificity
ELISA: this antibody diluted at 1:20,000 gave an O.D.= 1.0 in a 15 minute reaction against peptide conjugated to a different carrier than used for anti peptide purification. HRP conjugated goat anti chicken IgG (IgY) was used and TMB was the substrate.
- Light chain type
- unknown
- Concentration
- Purification notes
Antibodies were immunoaffinity purified using the peptide immobilized on a solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody (ab9111)
Immunofluorescent analysis of 4% paraformaldehyde (PFA)-fixed, permeabilised with 0.1% Triton X-100 in cells transfected with GFP-HA contrsucts (as CHO-GFP-HA) or parental non- transfected CHO cells labelling with chicken anti-HA tag (ab9111) at 5μg/ml, followed by Goat Anti-Chicken IgY (H&L), Alexa594 conjugated antibody at 2.5μg/ml (Red). Nucleus was counterstained with DAPI (Blue).
Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody (ab9111)
Immunofluorescent analysis of 4% paraformaldehyde (PFA) - fixed, permeabilized with 0.1% Triton X-100 CHO cells transfected with GFP-HA constructs (CHO-GFP-HA) labelling HA tag with ab9111 at 5μg/mL, followed by Goat Anti- Chicken IgY (H&L), Alexa594 conjugated antibody at 2.5 μg/mL (Red). Nucleus was counterstained with DAPI (Blue).
Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody (ab9111)
Immunofluorescent analysis of 4% paraformaldehyde (PFA) -fixed, permeabilized with 0.1% Triton X-100 CHO cells transfected with 12 tags constructs (CHO-12 tags) labelling HA tag with ab9111 at 1/250 dilution (4μg/mL), followed by Goat Anti-Chicken IgY (H&L), Alexa594 conjugated antibody at 1/500 dilution (2 μg/mL) (Red). Nucleus was counterstained with DAPI (white). Bottom panel images showed Parallel staining in parental CHO cell line.
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