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Rabbit anti-HA tag antibody ab9110 is a rabbit polyclonal antibody that is used in HA tag western blotting, immunofluorescence and flow cytometry.

Tried and trusted by researchers since 2002
Anti-HA tag antibody ab9110 is cited in over 1300 publications
Same trusted quality, New lower price!


Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Preservative: 0.1% Sodium azide
Constituents: PBS

Form

Liquid

Clonality

Polyclonal

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPChIPFlow CytELISAWBICC/IFChIP/Chip
Tag
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Tested
Tested

Species

Tag

Dilution info

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Notes

-

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Tested

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Tag

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Tag

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Notes

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species

Tag

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Tag

Dilution info

-

Notes

-

Tested
Tested

Species

Tag

Dilution info

-

Notes

-

Expected
Expected

Species

Tag

Dilution info

Use at an assay dependent concentration.

Notes

-

Alternative names

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Rabbit anti-HA tag antibody ab9110 is a rabbit polyclonal antibody that is used in HA tag western blotting, immunofluorescence and flow cytometry.

Tried and trusted by researchers since 2002
Anti-HA tag antibody ab9110 is cited in over 1300 publications
Same trusted quality, New lower price!

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Purification technique

Affinity purification Immunogen

Specificity

ELISA: The anti HA diluted 1:70.000 gave an O.D.=1.0 in a 15 minute reaction against peptide conjugated with a different carrier than used for anti peptide purification. HRP conjugated Goat anti rabbit IgG was used and TMB was the substrate.

Concentration
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Purification notes

Antibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

Activity summary

The HA tag also known as the hemagglutinin tag is a small peptide sequence derived from the human influenza virus hemagglutinin protein. Researchers typically use it in molecular biology to facilitate protein detection and purification. The tag consists of nine amino acids with a sequence YPYDVPDYA and a molecular weight of approximately 1.1 kDa. Scientists usually express the HA tag in recombinant proteins when they introduce it into cells or organisms allowing easy identification of the fused protein by specific antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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9 product images

  • Immunoprecipitation - Anti-HA tag antibody - ChIP Grade (ab9110), expandable thumbnail
    This image is courtesy of an anonymous customer review

    Immunoprecipitation - Anti-HA tag antibody - ChIP Grade (ab9110)

    ab9110 was diluted to 4 μg/mg lysate and incubated with a nuclear lysate of HEK293T cells transiently expressing HA-tagged protein and a Protein A matrix for 2 hours a 23°C to achieve immunoprecipitation. 1000 μg of lysate was present in the input.

    A HRP-conjugated anti-rabbit HA monoclonal antibody diluted 1/1000 was used for the Western Blot step.

    All lanes: Immunoprecipitation - Anti-HA tag antibody - ChIP Grade (AB9110)

    Predicted band size: 64 kDa

  • ChIP - Anti-HA tag antibody - ChIP Grade (ab9110), expandable thumbnail

    ChIP - Anti-HA tag antibody - ChIP Grade (ab9110)

    Xenopus laevis oocytes were injected with mRNA for HA-tagged human BORIS. Chromatin was prepared according to the Abcam X-ChIP protocol. Oocytes were fixed with formaldehyde for 10min. The ChIP was performed with 25μg of chromatin, 20μl of Protein A/G sepharose beads, and 3μg of ab9110 (anti-HA, light blue) or, 3μg of ab18337 (anti-Boris, dark blue). A non-specific antibody was used as a control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach).

  • ChIP - Anti-HA tag antibody - ChIP Grade (ab9110), expandable thumbnail
    This image is courtesy of a customer review submitted by Mr. Dan Stummer

    ChIP - Anti-HA tag antibody - ChIP Grade (ab9110)

    Chromatin was prepared according to the X-ChIP protocol. Mouse embryonic stem whole cell lysate treated with disuccinimidyl glutarate (cross-linking agent). ChIP was performed using ab9110 at 1/200 dilution for 16 hours at 4°C in RIPA diluent. The bound DNA was quantitated by real-time PCR. Negative control: The parent cell line. Positive control: A cell line, which stably express an HA-tagged RARgamma protein.

  • Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody - ChIP Grade (ab9110), expandable thumbnail
    This image is courtesy of a customer review

    Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody - ChIP Grade (ab9110)

    ab9110 staining HA-tagged proteins in HeLa cells by ICC/IF (Immunocytochemistry/immunoflurescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% saponin and blocked with 3% serum for 30 minutes at 37°C. Samples were incubated with primary antibody (2 μg/ml) in 1x PBS for 1 hour at 37°C. An Alexa Fluor® 488-conjugated Goat polyclonal to rabbit was used as secondary antibody.

  • Western blot - Anti-HA tag antibody - ChIP Grade (ab9110), expandable thumbnail

    Western blot - Anti-HA tag antibody - ChIP Grade (ab9110)

    This image is courtesy of an Abreview

    All lanes: Western blot - Anti-HA tag antibody - ChIP Grade (AB9110) at 1/4000 dilution

    Lane 1: 15ug untransfected wcl lysate

    Lane 2: 293FT cells transfected with 15kDa HA tagged Vpr (an HIV1 accessory protein)

    Secondary

    All lanes: HRP conjugated Goat anti-Rabbit

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 64 kDa

    Exposure time: 5s

  • Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody - ChIP Grade (ab9110), expandable thumbnail
    Seitz, K. et al Send to PLoS One. 2014 Jan 29;9(1):e87132. doi: 10.1371/journal.pone.0087132. eCollection 2014 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody - ChIP Grade (ab9110)

    Arrb2 depends on Gβγ to induce membrane translocation of PKCα

    Xenopus embryos were injected with 500 pg pkcα-gfp RNA and co-injected as indicated above the images. Animal Caps were prepared at stage 10 and immunostained as indicated. Nuclei were stained with Hoechst 33258 (blue). Images show representative results from at least two independent experiments with a minimum of six Animal Caps per experiment. Scale bars: 50 μm.

    The inhibitory effect of Arrb2 MO1 (E) on PKCα-GFP membrane translocation was rescued by (F) co-injection of HA-Gβ and HA-Gγ mRNA (anti-HA (red): F′, merge: F").

    HA was detected with ab9110.

    (After Figure 2 of Seitz et al)

  • Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody - ChIP Grade (ab9110), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody - ChIP Grade (ab9110)

    This image was kindly supplied as part of the review submitted by Kasper Fugger. Immunofluorescence staining of U-2 cells expressing HA-tagged protein with ab9110.

  • Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody - ChIP Grade (ab9110), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody - ChIP Grade (ab9110)

    ab 9110 at a 1/200 dilution staining the mouse olineu cell line (oligodendrocyte precursor cell) by immunocytochemistry. The antibody was incubated with the cells for 30 minutes and then detected using a Cy5 conjugated goat anti-rabbit antibody.

    This image is courtesy of an Abreview submitted by Katarina Trajkovic on 15 March 2006

  • Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody - ChIP Grade (ab9110), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody - ChIP Grade (ab9110)

    Immunofluorescent analysis of 4% paraformaldehyde (PFA) fixed, permeablised with 0.1% Triton X-100 CHO cells transfected with GFP-HA constructs (CHO-GFP-HA) labelling HA tag with ab9110 at 5μg/ml, followed by Donkey Anti-Rabbit IgG(H&L), Alexa 594 conjugated antibody at 2.5μg/ml (Red). Nucleus was counterstained with DAPI (Blue). Parallel staining in parental CHO cell line as negative control.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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