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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Rabbit anti-HA tag antibody ab9110 is a rabbit polyclonal antibody that is used in HA tag western blotting, immunofluorescence and flow cytometry.
Tried and trusted by researchers since 2002
Anti-HA tag antibody ab9110 is cited in over 1300 publications
Same trusted quality, New lower price!
IgG
Rabbit
Preservative: 0.1% Sodium azide
Constituents: PBS
Liquid
Polyclonal
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Species Tag | Dilution info - | Notes ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
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HA epitope tag, HA2, Hemagglutinin, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain
Rabbit anti-HA tag antibody ab9110 is a rabbit polyclonal antibody that is used in HA tag western blotting, immunofluorescence and flow cytometry.
Tried and trusted by researchers since 2002
Anti-HA tag antibody ab9110 is cited in over 1300 publications
Same trusted quality, New lower price!
HA epitope tag, HA2, Hemagglutinin, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain
IgG
Rabbit
Preservative: 0.1% Sodium azide
Constituents: PBS
Liquid
Polyclonal
Affinity purification Immunogen
ELISA: The anti HA diluted 1:70.000 gave an O.D.=1.0 in a 15 minute reaction against peptide conjugated with a different carrier than used for anti peptide purification. HRP conjugated Goat anti rabbit IgG was used and TMB was the substrate.
Antibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
The HA tag also known as the hemagglutinin tag is a small peptide sequence derived from the human influenza virus hemagglutinin protein. Researchers typically use it in molecular biology to facilitate protein detection and purification. The tag consists of nine amino acids with a sequence YPYDVPDYA and a molecular weight of approximately 1.1 kDa. Scientists usually express the HA tag in recombinant proteins when they introduce it into cells or organisms allowing easy identification of the fused protein by specific antibodies.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
ab9110 was diluted to 4 μg/mg lysate and incubated with a nuclear lysate of HEK293T cells transiently expressing HA-tagged protein and a Protein A matrix for 2 hours a 23°C to achieve immunoprecipitation. 1000 μg of lysate was present in the input.
A HRP-conjugated anti-rabbit HA monoclonal antibody diluted 1/1000 was used for the Western Blot step.
All lanes: Immunoprecipitation - Anti-HA tag antibody - ChIP Grade (AB9110)
Predicted band size: 64 kDa
Xenopus laevis oocytes were injected with mRNA for HA-tagged human BORIS. Chromatin was prepared according to the Abcam X-ChIP protocol. Oocytes were fixed with formaldehyde for 10min. The ChIP was performed with 25μg of chromatin, 20μl of Protein A/G sepharose beads, and 3μg of ab9110 (anti-HA, light blue) or, 3μg of ab18337 (anti-Boris, dark blue). A non-specific antibody was used as a control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach).
Chromatin was prepared according to the X-ChIP protocol. Mouse embryonic stem whole cell lysate treated with disuccinimidyl glutarate (cross-linking agent). ChIP was performed using ab9110 at 1/200 dilution for 16 hours at 4°C in RIPA diluent. The bound DNA was quantitated by real-time PCR. Negative control: The parent cell line. Positive control: A cell line, which stably express an HA-tagged RARgamma protein.
ab9110 staining HA-tagged proteins in HeLa cells by ICC/IF (Immunocytochemistry/immunoflurescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% saponin and blocked with 3% serum for 30 minutes at 37°C. Samples were incubated with primary antibody (2 μg/ml) in 1x PBS for 1 hour at 37°C. An Alexa Fluor® 488-conjugated Goat polyclonal to rabbit was used as secondary antibody.
This image is courtesy of an Abreview
All lanes: Western blot - Anti-HA tag antibody - ChIP Grade (AB9110) at 1/4000 dilution
Lane 1: 15ug untransfected wcl lysate
Lane 2: 293FT cells transfected with 15kDa HA tagged Vpr (an HIV1 accessory protein)
All lanes: HRP conjugated Goat anti-Rabbit
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 64 kDa
Exposure time: 5s
Arrb2 depends on Gβγ to induce membrane translocation of PKCα
Xenopus embryos were injected with 500 pg pkcα-gfp RNA and co-injected as indicated above the images. Animal Caps were prepared at stage 10 and immunostained as indicated. Nuclei were stained with Hoechst 33258 (blue). Images show representative results from at least two independent experiments with a minimum of six Animal Caps per experiment. Scale bars: 50 μm.
The inhibitory effect of Arrb2 MO1 (E) on PKCα-GFP membrane translocation was rescued by (F) co-injection of HA-Gβ and HA-Gγ mRNA (anti-HA (red): F′, merge: F").
HA was detected with ab9110.
(After Figure 2 of Seitz et al)
This image was kindly supplied as part of the review submitted by Kasper Fugger. Immunofluorescence staining of U-2 cells expressing HA-tagged protein with ab9110.
ab 9110 at a 1/200 dilution staining the mouse olineu cell line (oligodendrocyte precursor cell) by immunocytochemistry. The antibody was incubated with the cells for 30 minutes and then detected using a Cy5 conjugated goat anti-rabbit antibody.
This image is courtesy of an Abreview submitted by Katarina Trajkovic on 15 March 2006
Immunofluorescent analysis of 4% paraformaldehyde (PFA) fixed, permeablised with 0.1% Triton X-100 CHO cells transfected with GFP-HA constructs (CHO-GFP-HA) labelling HA tag with ab9110 at 5μg/ml, followed by Donkey Anti-Rabbit IgG(H&L), Alexa 594 conjugated antibody at 2.5μg/ml (Red). Nucleus was counterstained with DAPI (Blue). Parallel staining in parental CHO cell line as negative control.
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