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Anti-HA tag antibody [EPR22819-101] ab236632 is a rabbit monoclonal antibody that is used in HA tag western blotting, IHC, immunofluorescence and flow cytometry.

- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation
- One antibody for all your HA tag staining, use in HA tag western blotting, IHC, immunofluorescence and flow cytometry
Same trusted quality, new lower price


Images

Immunoprecipitation - Anti-HA tag antibody [EPR22819-101] (AB236632), expandable thumbnail
  • Western blot - Anti-HA tag antibody [EPR22819-101] (AB236632), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-HA tag antibody [EPR22819-101] (AB236632), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [EPR22819-101] (AB236632), expandable thumbnail
  • Western blot - Anti-HA tag antibody [EPR22819-101] (AB236632), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ChIPIPWBICC/IFFlow Cyt (Intra)IHC-P
Tag
Not recommended
Tested
Tested
Tested
Tested
Tested

Not recommended
Not recommended

Species

Tag

Dilution info

-

Notes

-

Tested
Tested

Species

Tag

Dilution info

1/30

Notes

-

Tested
Tested

Species

Tag

Dilution info

1/1000

Notes

-

Tested
Tested

Species

Tag

Dilution info

1/1000

Notes

-

Tested
Tested

Species

Tag

Dilution info

1/600

Notes

-

Tested
Tested

Species

Tag

Dilution info

-

Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization either through clathrin-dependent endocytosis or through clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.

Alternative names

Hemagglutinin, HA

Recommended products

Anti-HA tag antibody [EPR22819-101] ab236632 is a rabbit monoclonal antibody that is used in HA tag western blotting, IHC, immunofluorescence and flow cytometry.

- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation
- One antibody for all your HA tag staining, use in HA tag western blotting, IHC, immunofluorescence and flow cytometry
Same trusted quality, new lower price

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR22819-101

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Immunoprecipitation - Anti-HA tag antibody [EPR22819-101] (ab236632), expandable thumbnail

    Immunoprecipitation - Anti-HA tag antibody [EPR22819-101] (ab236632)

    HA tag was immunoprecipitated from 0.35 mg HEK-293T (human embryonic kidney) transfected with TIM 4 (WT) expression vector containing a HA-GFP-tag and PDGFR TM domain whole cell lysate 10μg with ab236632 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab236632. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/2000 dilution.

    Lane 1: HEK-293T (human embryonic kidney) transfected with TIM 4 (WT) expression vector containing a HA-GFP-tag and PDGFR TM domain whole cell lysate 10μg.

    Lane 2: ab236632 IP in HEK-293T transfected with TIM 4 (WT) expression vector containing a HA-GFP-tag and PDGFR TM domain whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab236632 in HEK-293T transfected with TIM 4 (WT) expression vector containing a HA-GFP-tag and PDGFR TM domain whole cell lysate.

    Blocking and dilution buffer and concentration/ 5% NFDM/TBST.

    Exposure time: 3 seconds.

    All lanes: Immunoprecipitation - Anti-HA tag antibody [EPR22819-101] (ab236632)

    Predicted band size: 64 kDa

  • Western blot - Anti-HA tag antibody [EPR22819-101] (ab236632), expandable thumbnail

    Western blot - Anti-HA tag antibody [EPR22819-101] (ab236632)

    Antibodies used in the control panels are (Anti-TIM 4 antibody), Anti-HA tag antibody - ChIP Grade ab9110 (Anti-HA tag antibody - ChIP Grade) and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Anti-GAPDH antibody [EPR16891] - Loading Control).

    Exposure time: 3 minutes.

    Blocking and Diluting Buffer and concentration: 5% NFDM/TBST.

    All lanes: Western blot - Anti-HA tag antibody [EPR22819-101] (ab236632) at 1/1000 dilution

    Lane 1: HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a HA-myc-His-GFP-tag and PDGFR TM domain, whole cell lysate at 10 µg

    Lane 2: HEK-293T transfected with TIM 4 (WT) expression vector containing a HA-myc-His-GFP-tag and PDGFR TM domain, whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 64 kDa

    Observed band size: 37 kDa, 78 kDa

  • Flow Cytometry (Intracellular) - Anti-HA tag antibody [EPR22819-101] (ab236632), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-HA tag antibody [EPR22819-101] (ab236632)

    Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HEK-293T (human embryonic kidney) transfected with TATA-box-binding protein (WT) expression vector containing HA-tag (Right) / HEK-293T (Left) cells labeling HA tag with ab236632 at 1/600 dilution. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [EPR22819-101] (ab236632), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [EPR22819-101] (ab236632)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) transfected with HA-tagged TATA-box-binding protein (WT) expression vector labeling HA tag with ab236632 at 1/1000 (0.6 μg/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000, (2 μg/ml) dilution (Green). Confocal image showing nuclear staining in HEK-293T cells transfected with HA-tagged TATA-box-binding protein (WT) expression vector is observed. Anti-HA.11 Epitope Tag antibody (Alexa Fluor® 647) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution.

  • Western blot - Anti-HA tag antibody [EPR22819-101] (ab236632), expandable thumbnail

    Western blot - Anti-HA tag antibody [EPR22819-101] (ab236632)

    Rfx Cas13d may cleave to two fragments close to C-term, and this antibody can recognize the full length and N-terminal fragment. HA tag antibody can recognize the full length and N-terminal fragment. His-tag antibody can recognize full length and C-terminal fragment because the His-tag of this overexpression vector located on C-term, HA-myc-tag of this overexpression vector located on N-term.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    In Western blot, Anti-HA tag antibody [EPR22819-101] - (ab236632) staining at 1/1000 dilution.

    In Western blot, Anti-6XHis tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

    All lanes: Western blot - Anti-Rfx Cas13d (N-term) antibody [EPR23965-31] (Anti-Rfx Cas13d (N-term) antibody [EPR23965-31] ab314741) at 1/1000 dilution

    Lane 1: 293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a myc-His-tag®, whole cell lysate at 10 µg

    Lane 2: 293T cells transfected with a Ruminococcus flavefaciens cas13d expression vector containing a HA-myc-tag (N-term) and a His-tag® (C-term), whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 110 kDa, 70 kDa, 36 kDa, 40 kDa

    Exposure time: 6s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HA tag antibody [EPR22819-101] (ab236632), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HA tag antibody [EPR22819-101] (ab236632)

    Immunohistochemical analysis of paraffin-embedded human tonsil with ab236632 at 1/5000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: No staining on human tonsil.

    The section was incubated with ab236632 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND RX instrument.

    Counterstained with Hematoxylin.

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HA tag antibody [EPR22819-101] (ab236632), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HA tag antibody [EPR22819-101] (ab236632)

    Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a HA Tag expression vector, (B) HEK-293T transfected with empty vector
    labelling HA tag with ab236632 at 1/5000 (0.124 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on HEK-293T transfected with a HA Tag expression vector (Image A) and no staining on (B) HEK-293T transfected with empty vector (Image B)


    The section was incubated with ab236632 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HA tag antibody [EPR22819-101] (ab236632), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HA tag antibody [EPR22819-101] (ab236632)

    Immunohistochemical analysis of paraffin-embedded Rat spleen with ab236632 at 1/5000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: No staining on Rat spleen.

    The section was incubated with ab236632 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND RX instrument.

    Counterstained with Hematoxylin.

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HA tag antibody [EPR22819-101] (ab236632), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HA tag antibody [EPR22819-101] (ab236632)

    Immunohistochemical analysis of paraffin-embedded Mouse spleen with ab236632 at 1/5000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: No staining on Mouse spleen.

    The section was incubated with ab236632 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND RX instrument.

    Counterstained with Hematoxylin.

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

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Product protocols

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