AB182009

Anti-HA tag antibody [EPR4095]

RabMAb
Recombinant
Lab Essentials
What is this?

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(6 Publications)

Rabbit Recombinant Monoclonal HA tag antibody. Suitable for WB and reacts with Tag samples. Cited in 6 publications.

View Alternative Names

HA epitope tag, HA2, Hemagglutinin, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain

2 Images

Supplier Data

Western blot - Anti-HA tag antibody [EPR4095] (AB182009)

Blocking/ Dilution buffer : 5% NFDM /TBST.

All lanes:

Western blot - Anti-HA tag antibody [EPR4095] (ab182009) at 1/5000 dilution

All lanes:

multi-tag fusion protein, 1 ng

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 64 kDa

false

Lab

Western blot - Anti-HA tag antibody [EPR4095] (AB182009)

All lanes:

Western blot - Anti-HA tag antibody [EPR4095] (ab182009) at 1/10000 dilution

All lanes:

HA-tagged Human Glutaminase C overexpression lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 64 kDa

Observed band size: 57 kDa

false

Carrier free

Anti-HA tag antibody [EPR4095] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4095

Isotype

IgG

Carrier free

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information HA tag

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Translational oncology 51:102181 PubMed39541710

2024

Methionine restriction promotes the polarization of macrophages towards M1 and the immunotherapy effect of PD-L1/PD-1 blockades by inhibiting the secretion of MIF by gastric carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Xin,Jiang Liu,Jun-Yan Lai,He-Song Xu,Luo-Jun Fan,Yong-Hui Zou,Qi Zhou,Zhen-Qi Yue,Jin-Heng Gan

Frontiers in cell and developmental biology 10:833396 PubMed35669517

2022

Differential Degradation of TRA2A and PYCR2 Mediated by Ubiquitin E3 Ligase E4B.

Applications

Unspecified application

Species

Unspecified reactive species

Yao Lu,Bo Jiang,Kangli Peng,Shasha Li,Xiangnan Liu,Bufan Wang,Yuntian Chen,Tiepeng Wang,Bo Zhao

Cell cycle (Georgetown, Tex.) 20:1692-1707 PubMed34334113

2021

ZEB2, interacting with MDM2, contributes to the dysfuntion of brain microvascular endothelial cells and brain injury after intracerebral hemorrhage.

Applications

Unspecified application

Species

Unspecified reactive species

Qingbao Guo,Manli Xie,Miao Guo,Feiping Yan,Lihong Li,Rui Liu

EMBO reports 21:e51869 PubMed33274589

2020

Atg1 kinase regulates autophagosome-vacuole fusion by controlling SNARE bundling.

Applications

Unspecified application

Species

Unspecified reactive species

Saskia Barz,Franziska Kriegenburg,Anna Henning,Anuradha Bhattacharya,Hector Mancilla,Pablo Sánchez-Martín,Claudine Kraft

Journal of cell science 132: PubMed31649143

2019

Vac8 spatially confines autophagosome formation at the vacuole in .

Applications

Unspecified application

Species

Unspecified reactive species

David M Hollenstein,Rubén Gómez-Sánchez,Akif Ciftci,Franziska Kriegenburg,Muriel Mari,Raffaela Torggler,Mariya Licheva,Fulvio Reggiori,Claudine Kraft

The Journal of biological chemistry 290:3529-41 PubMed25519908

2014

Proteolytic activation of the protease-activated receptor (PAR)-2 by the glycosylphosphatidylinositol-anchored serine protease testisin.

Applications

Unspecified application

Species

Human

Kathryn H Driesbaugh,Marguerite S Buzza,Erik W Martin,Gregory D Conway,Joseph P Y Kao,Toni M Antalis

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HA tag antibody [EPR4095]

Western blot - Anti-HA tag antibody [EPR4095] (AB182009)

Western blot - Anti-HA tag antibody [EPR4095] (AB182009)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (6)

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