Anti-HA tag antibody [HA-7] (ab49969) is a mouse monoclonal antibody that is used to detect HA tag in Western Blot, IP, ICC/IF, ELISA.
- Over 10 publications
- Trusted since 2007
Images
![Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA-7] (AB49969), expandable thumbnail](https://content.abcam.com/products/images/ha-tag-antibody-ha-7-ab49969--immunocytochemistry-immunofluorescence-img71713.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA-7] (AB49969)")
![Immunoprecipitation - Anti-HA tag antibody [HA-7] (AB49969), expandable thumbnail](https://content.abcam.com/products/images/ha-tag-antibody-ha-7-ab49969--immunoprecipitation-img89857.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-HA tag antibody [HA-7] (AB49969)")
![Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA-7] (AB49969), expandable thumbnail](https://content.abcam.com/products/images/ha-tag-antibody-ha-7-ab49969--immunocytochemistry-immunofluorescence-img42297.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA-7] (AB49969)")
Publications
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- Nature communications 14:51952023A super-enhancer-regulated RNA-binding protein cascade drives pancreatic cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCorina E Antal,Tae Gyu Oh,Stefan Aigner,En-Ching Luo,Brian A Yee,Tania Campos,Hervé Tiriac,Katherine L Rothamel,Zhang Cheng,Henry Jiao,Allen Wang,Nasun Hah,Elizabeth Lenkiewicz,Jan C Lumibao,Morgan L Truitt,Gabriela Estepa,Ester Banayo,Senada Bashi,Edgar Esparza,Ruben M Munoz,Jolene K Diedrich,Nicole M Sodir,Jasmine R Mueller,Cory R Fraser,Erkut Borazanci,David Propper,Daniel D Von Hoff,Christopher Liddle,Ruth T Yu,Annette R Atkins,Haiyong Han,Andrew M Lowy,Michael T Barrett,Dannielle D Engle,Gerard I Evan,Gene W Yeo,Michael Downes,Ronald M EvansPubMed 37673892
- Cell reports 42:1122442023A combinatorial approach to uncover an additional Integrator subunit.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSarah R Offley,Moritz M Pfleiderer,Avery Zucco,Angelique Fraudeau,Sarah A Welsh,Michal Razew,Wojciech P Galej,Alessandro GardiniPubMed 36920904
- Nature 595:404-4082021Mechanism of disease and therapeutic rescue of Dok7 congenital myasthenia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJulien Oury,Wei Zhang,Nadia Leloup,Akiko Koide,Alexis D Corrado,Gayatri Ketavarapu,Takamitsu Hattori,Shohei Koide,Steven J BurdenPubMed 34163073
- Oncology reports 46:2021Interaction between CD9 and PI3K‑p85 activates the PI3K/AKT signaling pathway in B‑lineage acute lymphoblastic leukemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYi-Fen Shi,Zi-Yang Huang,Yi-Sha Huang,Ru-Jiao Dong,Chong-Yun Xing,Kang Yu,Kam Tong Leung,Jian-Hua FengPubMed 34036396
- Cell chemical biology 26:1407-1416.e52019Split-miniSOG for Spatially Detecting Intracellular Protein-Protein Interactions by Correlated Light and Electron Microscopy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaniela Boassa et. al.PubMed 31378710
- PloS one 14:e02140352019Identification and characterization of putative Aeromonas spp. T3SS effectors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLuiz Thiberio Rangel et. al.PubMed 31163020
- The CRISPR journal 1:337-3472019Three-Component Repurposed Technology for Enhanced Expression: Highly Accumulable Transcriptional Activators via Branched Tag Arrays.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAtsushi Kunii,Yoshihiro Hara,Mitsumasa Takenaga,Naoko Hattori,Takuya Fukazawa,Toshikazu Ushijima,Takashi Yamamoto,Tetsushi SakumaPubMed 31021277
- Cell metabolism 29:1166-1181.e62019Deubiquitinases Maintain Protein Homeostasis and Survival of Cancer Cells upon Glutathione Depletion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIsaac S Harris et. al.PubMed 30799286
- Biochemistry and biophysics reports 16:115-1212018Onset of deaminase APOBEC3B induction in response to DNA double-strand breaks.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAtsuhiro Shimizu et. al.PubMed 30417129
- Nucleic acids research 46:4933-49492018Mbd2-CP2c loop drives adult-type globin gene expression and definitive erythropoiesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMin Young Kim,Ji Sook Kim,Seung Han Son,Chang Su Lim,Hea Young Eum,Dae Hyun Ha,Mi Ae Park,Eun Jung Baek,Buom-Yong Ryu,Ho Chul Kang,Vladimir N Uversky,Chul Geun KimPubMed 29547954
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS, 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Reactivity data
Select an application| IP | ELISA | WB | ICC/IF | |
|---|---|---|---|---|
| Tag | Tested | Expected | Expected | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info 0.5-2 µg/mL | Notes - |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info Use at an assay dependent concentration. | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info 0.25-1 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info 1-2 µg/mL | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Alternative names
HA epitope tag, HA2, Hemagglutinin, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain
Recommended products
Anti-HA tag antibody [HA-7] (ab49969) is a mouse monoclonal antibody that is used to detect HA tag in Western Blot, IP, ICC/IF, ELISA.
- Over 10 publications
- Trusted since 2007
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS, 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Clone number
- HA-7
- Purification technique
- Proprietary technique
- Concentration
- Purification notes
Purified from Tissue culture supernatant”.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-HA tag antibody [HA-7] (ab49969) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA.
Tagged protein detection
HA tag antibodies are used to visualize proteins labelled with this tag in a variety of applications.
What is the molecular weight of HA tag?
Anti-HA tag [HA-7] (ab49969) specifically detects a band for HA tag (UniProt: P03435) at a molecular weight of 63kDa.
Trusted by the scientific community
Anti-HA tag [HA-7] (ab49969) was first used in a scientific publication in 2007 and has been cited over 10 times in peer-reviewed journals.
Reviewed by scientists
Anti-HA tag [HA-7] (ab49969) has over 5 independent reviews from customers.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA-7] (ab49969), expandable thumbnail](https://content.abcam.com/products/images/ha-tag-antibody-ha-7-ab49969--immunocytochemistry-immunofluorescence-img71713.jpg "Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA-7] (ab49969)")
Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA-7] (ab49969)
HEK-293 cells overexpressing N-terminal HA tagged fusion protein were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton™ X-100. Fixed cells were stained with 1 μg/mL Anti-HA tag antibody [HA-7] (ab49969). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
![Immunoprecipitation - Anti-HA tag antibody [HA-7] (ab49969), expandable thumbnail](https://content.abcam.com/products/images/ha-tag-antibody-ha-7-ab49969--immunoprecipitation-img89857.jpg "Immunoprecipitation - Anti-HA tag antibody [HA-7] (ab49969)")
This image is courtesy of an anonymous customer reviewImmunoprecipitation - Anti-HA tag antibody [HA-7] (ab49969)
ab49969 Immunoprecipitating HA tag in HA tagged protein transfected 293T whole cell lysate. 25μg of cell lysate was incubated with primary antibody (1/2500 in lysis buffer) and matrix (Protein A/G) for 6 hours at 4°C. For western blotting anti-mouse HRP (Goat Anti-Mouse IgG H&L (HRP) ab6789) (1/10000) was used to confirm successful immunoprecipation.
75kD is specific band for HA tagged protein. 120kD, 55kD, 35kD is non specific band for protein A/G bead.
All lanes: Immunoprecipitation - Anti-HA tag antibody [HA-7] (ab49969)
Predicted band size: 64 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA-7] (ab49969), expandable thumbnail](https://content.abcam.com/products/images/ha-tag-antibody-ha-7-ab49969--immunocytochemistry-immunofluorescence-img42297.jpg "Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA-7] (ab49969)")
Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA-7] (ab49969)
HEK-293 cells overexpressing C-terminal HA tagged fusion protein were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton™ X-100. Fixed cells were stained with 1 μg/mL Anti-HA tag antibody [HA-7] (ab49969). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
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