Anti-HA tag antibody [HA.C5] ab18181 is a mouse monoclonal antibody that is used in HA tag western blotting and immunofluorescence.
- Antibody clone HA.C5 is the most widely used clone for HA tag on the market and is cited in >260 publications
Same trusted quality, new lower price
IgG3
Mouse
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: PBS
Liquid
Monoclonal
WB | ICC/IF | |
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Tag | Tested | Tested |
Species | Dilution info | Notes |
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Species Tag | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
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Species Tag | Dilution info - | Notes - |
Select an associated product type
HA epitope tag, HA2, Hemagglutinin, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain
Anti-HA tag antibody [HA.C5] ab18181 is a mouse monoclonal antibody that is used in HA tag western blotting and immunofluorescence.
- Antibody clone HA.C5 is the most widely used clone for HA tag on the market and is cited in >260 publications
Same trusted quality, new lower price
IgG3
Mouse
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: PBS
Liquid
Monoclonal
HA.C5
Affinity purification
Purified from TCS
Blue Ice
+4°C
+4°C
Do Not Freeze
This product was changed from ascites to tissue culture supernatant on 5th February 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
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We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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Full details and terms and conditions can be found here:
Terms & Conditions.
All lanes: Western blot - Anti-HA tag antibody [HA.C5] (ab18181) at 1/1000 dilution
Lanes 1 - 2: HEK293 whole cell lysate - transfected at 30 µg
Lane 3: HEK293 whole cell lysate - untransfected at 30 µg
All lanes: IRDye® 800CW Goat anti-mouse IgG polyclonal at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 64 kDa
Observed band size: 85 kDa
Exposure time: 5min
ab18181 staining HA tag (green) in HeLa cells by Immunocytochemistry/ Immunofluorescence.
Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 3% BSA for 1 hour at 22°C. Samples were incubated with primary antibody (1/1000 in diluent) for 1 hour at 22°C. A FITC-conjugated goat anti-mouse polyclonal IgG (1/1000) was used as the secondary antibody. Nuclei were stained with DAPI (blue).
Incubation with the primary antibody was carried out at 4°C overnight, whilst the secondary antibody was incubated for 1 hour at room temperature.
All lanes: Western blot - Anti-HA tag antibody [HA.C5] (ab18181) at 1/2000 dilution
Lane 1: WCE from cell line transfected for HA-tagged protein at 50 µg
Lane 2: WCE from a cell line transfected with empty vector at 50 µg
All lanes: HRP conjugated Goat anti-mouse IgG (H+L)
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 64 kDa
Exposure time: 10s
Western blot using ab18181 of 293 cells transfected with HA-tagged vector(2) and untransfected control (1). Western blot using ab18181 of 293 cells transfected with HA-tagged vector(2) and untransfected control (1).
All lanes: Western blot - Anti-HA tag antibody [HA.C5] (ab18181)
Predicted band size: 64 kDa
Immunofluorescence using ab18181 staining a HA-tag fusion protein (transcription factor) in a stable expressing cell line (right hand panel) and control cell line (left hand panel).
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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