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Anti-HA tag antibody [HA.C5] ab18181 is a mouse monoclonal antibody that is used in HA tag western blotting and immunofluorescence.

- Antibody clone HA.C5 is the most widely used clone for HA tag on the market and is cited in >260 publications
Same trusted quality, new lower price


Images

Western blot - Anti-HA tag antibody [HA.C5] (AB18181), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA.C5] (AB18181), expandable thumbnail
  • Western blot - Anti-HA tag antibody [HA.C5] (AB18181), expandable thumbnail
  • Western blot - Anti-HA tag antibody [HA.C5] (AB18181), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA.C5] (AB18181), expandable thumbnail

Publications

Key facts

Isotype

IgG3

Host species

Mouse

Storage buffer

pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Tag
Tested
Tested

Tested
Tested

Species

Tag

Dilution info

1/1000

Notes

-

Tested
Tested

Species

Tag

Dilution info

-

Notes

-

Associated Products

Select an associated product type

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Alternative names

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Anti-HA tag antibody [HA.C5] ab18181 is a mouse monoclonal antibody that is used in HA tag western blotting and immunofluorescence.

- Antibody clone HA.C5 is the most widely used clone for HA tag on the market and is cited in >260 publications
Same trusted quality, new lower price

Key facts

Isotype

IgG3

Form

Liquid

Clonality

Monoclonal

Clone number

HA.C5

Purification technique

Affinity purification

Concentration
Loading...
Purification notes

Purified from TCS

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

This product was changed from ascites to tissue culture supernatant on 5th February 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-HA tag antibody [HA.C5] (ab18181), expandable thumbnail
    This image is courtesy of an anonymous customer review

    Western blot - Anti-HA tag antibody [HA.C5] (ab18181)

    All lanes: Western blot - Anti-HA tag antibody [HA.C5] (ab18181) at 1/1000 dilution

    Lanes 1 - 2: HEK293 whole cell lysate - transfected at 30 µg

    Lane 3: HEK293 whole cell lysate - untransfected at 30 µg

    Secondary

    All lanes: IRDye® 800CW Goat anti-mouse IgG polyclonal at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 64 kDa

    Observed band size: 85 kDa

    Exposure time: 5min

  • Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA.C5] (ab18181), expandable thumbnail
    This image is courtesy of a customer review submitted by Harendra Chahar

    Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA.C5] (ab18181)

    ab18181 staining HA tag (green) in HeLa cells by Immunocytochemistry/ Immunofluorescence.

    Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 3% BSA for 1 hour at 22°C. Samples were incubated with primary antibody (1/1000 in diluent) for 1 hour at 22°C. A FITC-conjugated goat anti-mouse polyclonal IgG (1/1000) was used as the secondary antibody. Nuclei were stained with DAPI (blue).

  • Western blot - Anti-HA tag antibody [HA.C5] (ab18181), expandable thumbnail
    This image is courtesy of an anonymous customer review

    Western blot - Anti-HA tag antibody [HA.C5] (ab18181)

    Incubation with the primary antibody was carried out at 4°C overnight, whilst the secondary antibody was incubated for 1 hour at room temperature.

    All lanes: Western blot - Anti-HA tag antibody [HA.C5] (ab18181) at 1/2000 dilution

    Lane 1: WCE from cell line transfected for HA-tagged protein at 50 µg

    Lane 2: WCE from a cell line transfected with empty vector at 50 µg

    Secondary

    All lanes: HRP conjugated Goat anti-mouse IgG (H+L)

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 64 kDa

    Exposure time: 10s

  • Western blot - Anti-HA tag antibody [HA.C5] (ab18181), expandable thumbnail

    Western blot - Anti-HA tag antibody [HA.C5] (ab18181)

    Western blot using ab18181 of 293 cells transfected with HA-tagged vector(2) and untransfected control (1). Western blot using ab18181 of 293 cells transfected with HA-tagged vector(2) and untransfected control (1).

    All lanes: Western blot - Anti-HA tag antibody [HA.C5] (ab18181)

    Predicted band size: 64 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA.C5] (ab18181), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA.C5] (ab18181)

    Immunofluorescence using ab18181 staining a HA-tag fusion protein (transcription factor) in a stable expressing cell line (right hand panel) and control cell line (left hand panel).

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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