Anti-HA tag antibody [HA.C5]

• WB

AbReview39578****

Western blot - Anti-HA tag antibody [HA.C5] (AB18181)

All lanes:

Western blot - Anti-HA tag antibody [HA.C5] (ab18181) at 1/1000 dilution

Lanes 1 - 2:

HEK293 whole cell lysate - transfected at 30 µg

Lane 3:

HEK293 whole cell lysate - untransfected at 30 µg

Secondary

All lanes:

IRDye® 800CW Goat anti-mouse IgG polyclonal at 1/10000 dilution

Predicted band size: 64 kDa

Observed band size: 85 kDa

false

Exposure time: 5min

This image is courtesy of an anonymous Abreview

• ICC/IF

AbReview31652****

Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA.C5] (AB18181)

ab18181 staining HA tag (green) in HeLa cells by Immunocytochemistry/ Immunofluorescence.

Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 3% BSA for 1 hour at 22°C. Samples were incubated with primary antibody (1/1000 in diluent) for 1 hour at 22°C. A FITC-conjugated goat anti-mouse polyclonal IgG (1/1000) was used as the secondary antibody. Nuclei were stained with DAPI (blue).

This image is courtesy of an Abreview submitted by Harendra Chahar

• WB

Unknown

Western blot - Anti-HA tag antibody [HA.C5] (AB18181)

Western blot using ab18181 of 293 cells transfected with HA-tagged vector(2) and untransfected control (1). Western blot using ab18181 of 293 cells transfected with HA-tagged vector(2) and untransfected control (1).

All lanes:

Western blot - Anti-HA tag antibody [HA.C5] (ab18181)

Predicted band size: 64 kDa

false

• WB

AbReview5311****

Western blot - Anti-HA tag antibody [HA.C5] (AB18181)

Incubation with the primary antibody was carried out at 4°C overnight, whilst the secondary antibody was incubated for 1 hour at room temperature.

All lanes:

Western blot - Anti-HA tag antibody [HA.C5] (ab18181) at 1/2000 dilution

Lane 1:

WCE from cell line transfected for HA-tagged protein at 50 µg

Lane 2:

WCE from a cell line transfected with empty vector at 50 µg

Secondary

All lanes:

HRP conjugated Goat anti-mouse IgG (H+L)

Predicted band size: 64 kDa

true

Exposure time: 10s

This image is courtesy of an anonymous Abreview

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA.C5] (AB18181)

Immunofluorescence using ab18181 staining a HA-tag fusion protein (transcription factor) in a stable expressing cell line (right hand panel) and control cell line (left hand panel).