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Rabbit Recombinant Monoclonal HA tag antibody. Carrier free. Suitable for I-ELISA, IHC-P, ICC/IF, WB and reacts with Tag samples.

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Images

Western blot - Anti-HA tag antibody [HL1603] (AB308398), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HA tag antibody [HL1603] (AB308398), expandable thumbnail
  • Indirect ELISA - Anti-HA tag antibody [HL1603] (AB308398), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HL1603] (AB308398), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
I-ELISAIHC-PICC/IFWB
Tag
Tested
Tested
Tested
Tested

Tested
Tested

Species
Tag
Dilution info
1 µg/mL
Notes

-

Tested
Tested

Species
Tag
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species
Tag
Dilution info
1/500
Notes

-

Tested
Tested

Species
Tag
Dilution info
1/5000
Notes

-

Target data

Alternative names

HA epitope tag, HA2, Hemagglutinin, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain

Recommended products

Rabbit Recombinant Monoclonal HA tag antibody. Carrier free. Suitable for I-ELISA, IHC-P, ICC/IF, WB and reacts with Tag samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Carrier free
Yes
Clone number
HL1603
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-HA tag antibody [HL1603] (ab308398), expandable thumbnail

    Western blot - Anti-HA tag antibody [HL1603] (ab308398)

    Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with 6X His tag antibody [HL1603] (ab308398) diluted at 1/5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

    All lanes: Western blot - Anti-HA tag antibody [HL1603] (ab308398) at 1/5000 dilution

    Lane 1: Non-transfected (–) 293T whole cell extracts at 30 µg

    Lane 2: Transfected (+) 293T whole cell extracts at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HA tag antibody [HL1603] (ab308398), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HA tag antibody [HL1603] (ab308398)

    Immunohistochemical analysis of mock and HA tag - transfected HEK293T cell pellet labeling HA tag using ab308398 at 1/1000 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min

  • Indirect ELISA - Anti-HA tag antibody [HL1603] (ab308398), expandable thumbnail

    Indirect ELISA - Anti-HA tag antibody [HL1603] (ab308398)

    Indirect ELISA analysis was performed by coating the plate with recombinant HA-tag tagged protein (1000-15.63 ng/mL). Coated protein was probed with HA tag antibody [HL1603] (ab308398) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (1/10000) was used to detect the bound primary antibody.

  • Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HL1603] (ab308398), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HL1603] (ab308398)

    For immunofluorescence analysis, mock and transfected HEK293T cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes and permeabilized for detection of HA tag using ab308398 at 1/500 dilution (green). Blue: Fluoroshield with DAPI.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com