Anti-HA tag antibody [RM1058] - BSA and Azide free (ab314238)

Rabbit Recombinant Multiclonal Hemagglutinin antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra), IP, ChIP and reacts with Tag samples.

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Images

Flow Cytometry (Intracellular) - Anti-HA tag antibody [RM1058] - BSA and Azide free (AB314238), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Multiclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	IHC-P	ICC/IF	Flow Cyt (Intra)	IP	ChIP
Tag	Tested	Tested	Tested	Tested	Tested	Tested

Tested
Tested

Species	Dilution info	Notes
Species Tag	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Tag	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Tag	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Tag	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Tag	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Tag	Dilution info -	Notes -

Associated Products

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Target data

See full target information HA

Function

Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore. Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization either through clathrin-dependent endocytosis or through clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.

Alternative names

Hemagglutinin, HA

Recommended products

Rabbit Recombinant Multiclonal Hemagglutinin antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra), IP, ChIP and reacts with Tag samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Multiclonal
Immunogens: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: RM1058
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Notes

This product is a recombinant multiclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

Flow Cytometry (Intracellular) - Anti-HA tag antibody [RM1058] - BSA and Azide free (ab314238)
This data was developed using Anti-HA tag antibody [RM1058] ab314237, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Isotype (Left) / 293T cells transfected with a human HA Tag expression vector containing a GFP tag (Middle) / 293T cells transfected with an empty expression vector containing a GFP tag (Right) cells labelling HA tag with Anti-HA tag antibody [RM1058] ab314237 at 1/5000 dilution (0.01 ug)/Middle and Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control.
Goat anti-Rabbit IgG (Alexa Fluor® 647, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed ab150083) at 1/5000 dilution was used as the secondary antibody.
ChIP - Anti-HA tag antibody [RM1058] - BSA and Azide free (ab314238)
This data was developed using Anti-HA tag antibody [RM1058] ab314237, the same antibody clone in a different buffer formulation.
Chromatin was prepared from Hela (human epithelial cell line from cervix adenocarcinoma) transfected with HA Tagged human CREBBP and Hela transfected with empty vector cells according to the Abcam Dual-X-ChIP protocol*.Cells were fixed with 1.5 mM EGS for 30mins and then formaldehyde for 10min.
The ChIP was performed with 25 µg of chromatin, 5 µg of Anti-HA tag antibody [RM1058] ab314237 (red), or 5 µg of rabbit normal IgG Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 (gray) and 25 µl of Protein A/G Dynabeads. The immunoprecipitated DNA was quantified by real time PCR (SYBR green approach).
*http://www.abcam.com/resources?keywords=X%20ChIP%20protocol
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HA tag antibody [RM1058] - BSA and Azide free (ab314238)
This data was developed using Anti-HA tag antibody [RM1058] ab314237, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling HA tag with Anti-HA tag antibody [RM1058] ab314237 at 1/5000 (0.104 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on rat spleen.

The section was incubated with Anti-HA tag antibody [RM1058] ab314237 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HA tag antibody [RM1058] - BSA and Azide free (ab314238)
This data was developed using Anti-HA tag antibody [RM1058] ab314237, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling HA tag with Anti-HA tag antibody [RM1058] ab314237 at 1/5000 (0.104 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on human tonsil.

The section was incubated with Anti-HA tag antibody [RM1058] ab314237 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HA tag antibody [RM1058] - BSA and Azide free (ab314238)
This data was developed using Anti-HA tag antibody [RM1058] ab314237, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling HA tag with Anti-HA tag antibody [RM1058] ab314237 at 1/5000 (0.104 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on mouse spleen.

The section was incubated with Anti-HA tag antibody [RM1058] ab314237 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [RM1058] - BSA and Azide free (ab314238)
This data was developed using Anti-HA tag antibody [RM1058] ab314237, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293T (human epithelial cell line from embryonic kidney) transfected with GFP-tagged human HA Tag expression vector cells labelling HA tag with Anti-HA tag antibody [RM1058] ab314237 at 1/500 (1.042 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) antibody at 1/1000 (2 ug/mL) dilution (Green).
Confocal image showing cytoplasmic staining in 293T cells transfected with a human HA Tag expression vector containing a GFP tag.

Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).
The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/1000 (2 ug/mL) dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HA tag antibody [RM1058] - BSA and Azide free (ab314238)
This data was developed using Anti-HA tag antibody [RM1058] ab314237, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a HA Tag expression vector. (B) HEK-293T transfected with empty vector. tissue labeling HA tag with Anti-HA tag antibody [RM1058] ab314237 at 1/5000 (0.104 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a HA Tag expression vector. No staining on (B) HEK-293T transfected with empty vector.

The section was incubated with Anti-HA tag antibody [RM1058] ab314237 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Western blot - Anti-HA tag antibody [RM1058] - BSA and Azide free (ab314238)
This data was developed using Anti-HA tag antibody [RM1058] ab314237, the same antibody clone in a different buffer formulation.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
In Western blot, Anti-GFP antibody [E385] (Anti-GFP antibody [E385] ab32146) staining at 1/10000 dilution.
In Western blot, Anti-TIM 4 antibody [EPR22304-3] (Anti-TIM 4 antibody [EPR22304-3] ab222093) staining at 1/1000 dilution.
All lanes: Western blot - Anti-HA tag antibody [RM1058] (Anti-HA tag antibody [RM1058] ab314237) at 1/1000 dilution
Lane 1: 293T (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-GFP-tag, whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 2: 293T transfected with TIM 4 (WT) expression vector containing a HA-myc-His-GFP-tag and PDGFR TM domain, whole cell lysate at 20 µg with 5% NFDM/TBST
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 78 kDa, 36 kDa
Exposure time: 37s
Immunoprecipitation - Anti-HA tag antibody [RM1058] - BSA and Azide free (ab314238)
This data was developed using Anti-HA tag antibody [RM1058] ab314237, the same antibody clone in a different buffer formulation.
HA tag was immunoprecipitated from 0.35 mg 293T (human embryonic kidney) transfected with TIM 4 (WT) expression vector containing a HA-myc-His-GFP-tag and PDGFR TM domain, whole cell lysate with Anti-HA tag antibody [RM1058] ab314237 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-HA tag antibody [RM1058] ab314237 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: 293T (human embryonic kidney) transfected with TIM 4 (WT) expression vector containing a HA-myc-His-GFP-tag and PDGFR TM domain, whole cell lysate

Lane 2: Anti-HA tag antibody [RM1058] ab314237 IP in 293T (human embryonic kidney) transfected with TIM 4 (WT) expression vector containing a HA-myc-His-GFP-tag and PDGFR TM domain, whole cell lysate

Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-HA tag antibody [RM1058] ab314237 in 293T transfected with TIM 4 (WT) expression vector containing a HA-myc-His-GFP-tag and PDGFR TM domain, whole cell lysate
All lanes: Immunoprecipitation - Anti-HA tag antibody [RM1058] (Anti-HA tag antibody [RM1058] ab314237) at 1/30 dilution
All lanes: 293T (human embryonic kidney) transfected with TIM 4 (WT) expression vector containing a HA-myc-His-GFP-tag and PDGFR TM domain, whole cell lysate with 5% NFDM/TBST
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 8s