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AB210953

Anti-HA1(H5N1) antibody [2B7]

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(1 Publication)

Mouse Monoclonal HA1(H5N1) antibody. Suitable for WB and reacts with Influenza A samples. Cited in 1 publication.

View Alternative Names

Hemagglutinin A1

2 Images
Western blot - Anti-HA1(H5N1) antibody [2B7] (AB210953)
  • WB

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Western blot - Anti-HA1(H5N1) antibody [2B7] (AB210953)

All lanes:

Western blot - Anti-HA1(H5N1) antibody [2B7] (ab210953) at 1/1000 dilution

Lane 1:

H1N1 recombinant protein at 0.05 µg

Lane 2:

H3N2 recombinant protein at 0.05 µg

Lane 3:

H5N1/HA1 recombinant protein at 0.05 µg

Secondary

All lanes:

goat anti-mouse secondary antibody conjugated to HRP

false

Western blot - Anti-HA1(H5N1) antibody [2B7] (AB210953)
  • WB

Supplier Data

Western blot - Anti-HA1(H5N1) antibody [2B7] (AB210953)

This image was produced using the ascites version of this antibody.

All lanes:

Western blot - Anti-HA1(H5N1) antibody [2B7] (ab210953) at 1/3000 dilution

Lane 1:

HA1(H1N1) recombinant protein at 0.05 µg

Lane 2:

HA1(H3N2) recombinant protein at 0.05 µg

Lane 3:

HA1(H5N1) recombinant protein at 0.05 µg

Secondary

All lanes:

goat anti-mouse HRP

true

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2B7

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Influenza A

Applications

WB

applications

Specificity

ab210953 specifically recognizes HA1(H5N1) recombinant protein, but not interacted HA1(H1N1) and HA1(H3N2) recombinant protein in ELISA. Cross-reactivity has not been tested in other H5N1 types.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Influenza A": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

This product was changed from ascites to tissue culture supernatant on 24/01/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 10% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HA1 also known as Hemagglutinin subtype 1 (H5N1) is a glycoprotein with an important role in the influenza virus lifecycle specifically avian influenza H5N1. It has a mass of approximately 63 kDa. The primary mechanical function of HA1 is to mediate viral entry into host cells. It facilitates this by binding to sialic acid receptors on the host cell surface which enables viral attachment and subsequent membrane fusion. HA1 is expressed on the surface of the influenza virus and undergoes conformational changes essential for viral fusion and entry.
Biological function summary

HA1 functions within the viral spike complex known as the Hemagglutinin spike protein which includes HA2. This complex is responsible for the intricate process of virus attachment and entry into host cells. HA1's role in receptor binding is critical for this process making it essential for viral infectivity and host specificity. The precise interaction between HA1 and host cell receptors determines the host range of the influenza virus and is an important factor in cross-species transmission events.

Pathways

HA1 plays a fundamental role in the influenza infection pathway particularly in the receptor-mediated endocytosis of the virus. It works closely with neuraminidase another influenza surface protein which facilitates viral release from infected cells after replication. The balance between HA1's receptor binding and neuraminidase's receptor cleaving activities allows for efficient viral propagation and spread. These interactions highlight HA1's integral part in the viral lifecycle and pathogenicity.

HA1 is central to the pathogenesis of avian influenza H5N1 a highly pathogenic avian influenza strain that impacts both avian and mammalian hosts including humans. This strain can lead to severe respiratory illness and high mortality rates in infected individuals. The interaction of HA1 with host immune responses influences disease severity and outcome. In addition HA1 has potential implications in vaccine development as its antigenic properties are often targeted to induce protective immunity against influenza.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information HA1(H5N1)
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cells 11: PubMed35159261

2022

MicroRNA-100 Reduced Fetal Bovine Muscle Satellite Cell Myogenesis and Augmented Intramuscular Lipid Deposition by Modulating IGF1R.

Applications

Unspecified application

Species

Unspecified reactive species

Bilal Ahmad Mir,Elke Albrecht,Asghar Ali,Ola Hansson,Steffen Maak
View all publications
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Product promise

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