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AB181837

Anti-HABP2 antibody [EPR14551]

5

(1 Review)

|

(5 Publications)

Rabbit Recombinant Monoclonal HABP2 antibody. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 5 publications.

View Alternative Names

HGFAL, HABP2, Factor VII-activating protease, FSAP, FVII activator, Hepatocyte growth factor activator-like protein, Hyaluronan-binding protein 2, Plasma hyaluronan-binding protein, Plasma hyaluronan-binding serine protease, PHBP, PHBSP

2 Images
Flow Cytometry (Intracellular) - Anti-HABP2 antibody [EPR14551] (AB181837)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-HABP2 antibody [EPR14551] (AB181837)

Intracellular Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling HABP2 with purified ab181837 at 1/20 dilution (10 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

Western blot - Anti-HABP2 antibody [EPR14551] (AB181837)
  • WB

Unknown

Western blot - Anti-HABP2 antibody [EPR14551] (AB181837)

All lanes:

Western blot - Anti-HABP2 antibody [EPR14551] (ab181837) at 1/10000 dilution

Lane 1:

MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

A549 (Human lung carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

Mouse brain lysate at 20 µg

Lane 4:

Mouse heart lysate at 20 µg

Lane 5:

Rat brain lysate at 20 µg

Lane 6:

Rat heart lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/20000 dilution

Predicted band size: 63 kDa

Observed band size: 75 kDa

false

  • Carrier free

    Anti-HABP2 antibody [EPR14551] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14551

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Alternative Products

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p><strong>For unpurified use at 1/100.</strong></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "<p><strong>For unpurified use at 1/10000 - 1/50000.</strong></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/20", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "<p><strong>For unpurified use at 1/10000 - 1/50000.</strong></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "<p><strong>For unpurified use at 1/10000 - 1/50000.</strong></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Hyaluronan-Binding Protein 2 (HABP2) also known as Factor VII activating protease is a serine protease with an approximate mass of 65 kDa. This protein gets expressed mainly in the liver and secreted into the bloodstream. HABP2 participates in extracellular matrix interactions by binding to hyaluronan. It plays a role in coagulation processes. The protein also influences angiogenesis highlighting its mechanical involvement in vascular biology.
Biological function summary

HABP2 functions as a regulator in the maintenance of blood homeostasis and tissue remodeling. It takes part in fibrinolysis the process that prevents blood clots from growing and becoming problematic. HABP2 works together with fibrin to facilitate clot breakdown. It may also interact with plasminogen activators within these complexes to accomplish its biological functions effectively.

Pathways

HABP2 integrates into the fibrinolytic and coagulation pathways. It contributes to ensuring that the balance between clot formation and breakdown is maintained. The protein shows interactions with other components like urokinase-type plasminogen activator (uPA) which also plays a role in fibrinolysis. This highlights HABP2's participation in processes that involve preventing thrombosis.

HABP2 has associations with cardiovascular disorders including venous thromboembolism. Aberrant activity of HABP2 may contribute to an increased risk of thrombosis. The protein's interaction with plasminogen and fibrin suggests its involvement in abnormal clotting disorders. Further study into HABP2's role could aid in understanding its potential as a therapeutic target in these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cleaves the alpha-chain at multiple sites and the beta-chain between 'Lys-53' and 'Lys-54' but not the gamma-chain of fibrinogen and therefore does not initiate the formation of the fibrin clot and does not cause the fibrinolysis directly (PubMed : 11217080). It does not cleave (activate) prothrombin and plasminogen but converts the inactive single chain urinary plasminogen activator (pro-urokinase) to the active two chain form (PubMed : 10754382, PubMed : 11217080). Activates coagulation factor VII (Probable). May function as a tumor suppressor negatively regulating cell proliferation and cell migration (PubMed : 26222560).
See full target information HABP2
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:8036 PubMed39271701

2024

A FAPα-activated MRI nanoprobe for precise grading diagnosis of clinical liver fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jiahao Gao,Ya Wang,Xianfu Meng,Xiaoshuang Wang,Fang Han,Hao Xing,Guanglei Lv,Li Zhang,Shiman Wu,Xingwu Jiang,Zhenwei Yao,Xiangming Fang,Jiawen Zhang,Wenbo Bu

European cells & materials 41:142-152 PubMed33533018

2021

The effect of hyaluronic acid on nucleus pulposus extracellular matrix production through hypoxia-inducible factor-1α transcriptional activation of CD44 under hypoxia.

Applications

Unspecified application

Species

Unspecified reactive species

F Zhang,X Liu,B Li,Z Li,S Grad,D Chen,M Gao,S Liu

Journal of extracellular vesicles 8:1670893 PubMed31632619

2019

Degradation of tumour stromal hyaluronan by small extracellular vesicle-PH20 stimulates CD103 dendritic cells and in combination with PD-L1 blockade boosts anti-tumour immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Yeonsun Hong,Yoon Kyoung Kim,Gi Beom Kim,Gi-Hoon Nam,Seong A Kim,Yoon Park,Yoosoo Yang,In-San Kim

Clinical endocrinology 86:837-844 PubMed28222214

2017

Segregation and expression analyses of hyaluronan-binding protein 2 (HABP2): insights from a large series of familial non-medullary thyroid cancers and literature review.

Applications

Unspecified application

Species

Unspecified reactive species

Carla Colombo,Marina Muzza,Maria Carla Proverbio,Giulia Ercoli,Michela Perrino,Valentina Cirello,Leonardo Vicentini,Stefano Ferrero,Laura Fugazzola

International journal of molecular sciences 17: PubMed27347945

2016

Hypoxia Potentiates Anabolic Effects of Exogenous Hyaluronic Acid in Rat Articular Cartilage.

Applications

Unspecified application

Species

Unspecified reactive species

Shohei Ichimaru,Shuji Nakagawa,Yuji Arai,Tsunao Kishida,Masaharu Shin-Ya,Kuniaki Honjo,Shinji Tsuchida,Hiroaki Inoue,Hiroyoshi Fujiwara,Seiji Shimomura,Osam Mazda,Toshikazu Kubo
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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