Rabbit Recombinant Monoclonal HABP2 antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.
Images
![Western blot - Anti-HABP2 antibody [EPR14551] - BSA and Azide free (AB250514), expandable thumbnail](https://content.abcam.com/products/images/habp2-antibody-epr14551-bsa-and-azide-free-ab250514--western-blot-img144992.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-HABP2 antibody [EPR14551] - BSA and Azide free (AB250514)")
![Flow Cytometry (Intracellular) - Anti-HABP2 antibody [EPR14551] - BSA and Azide free (AB250514), expandable thumbnail](https://content.abcam.com/products/images/habp2-antibody-epr14551-bsa-and-azide-free-ab250514--flow-cytometry-intracellular-img80186.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-HABP2 antibody [EPR14551] - BSA and Azide free (AB250514)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | WB | IHC-P | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Tested |
| Mouse | Expected | Tested | Not recommended | Expected |
| Rat | Expected | Tested | Not recommended | Expected |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Cleaves the alpha-chain at multiple sites and the beta-chain between 'Lys-53' and 'Lys-54' but not the gamma-chain of fibrinogen and therefore does not initiate the formation of the fibrin clot and does not cause the fibrinolysis directly. It does not cleave (activate) prothrombin and plasminogen but converts the inactive single chain urinary plasminogen activator (pro-urokinase) to the active two chain form. Activates coagulation factor VII (PubMed:10754382, PubMed:11217080, PubMed:8827452). May function as a tumor suppressor negatively regulating cell proliferation and cell migration (PubMed:26222560).
Alternative names
HGFAL, PHBP, HABP2, Hyaluronan-binding protein 2, Factor VII-activating protease, Factor seven-activating protease, Hepatocyte growth factor activator-like protein, Plasma hyaluronan-binding protein, FSAP
Recommended products
Rabbit Recombinant Monoclonal HABP2 antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR14551
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab250514 is the carrier-free version of Anti-HABP2 antibody [EPR14551] ab181837.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Hyaluronan-Binding Protein 2 (HABP2) also known as Factor VII activating protease is a serine protease with an approximate mass of 65 kDa. This protein gets expressed mainly in the liver and secreted into the bloodstream. HABP2 participates in extracellular matrix interactions by binding to hyaluronan. It plays a role in coagulation processes. The protein also influences angiogenesis highlighting its mechanical involvement in vascular biology.
- Biological function summary
HABP2 functions as a regulator in the maintenance of blood homeostasis and tissue remodeling. It takes part in fibrinolysis the process that prevents blood clots from growing and becoming problematic. HABP2 works together with fibrin to facilitate clot breakdown. It may also interact with plasminogen activators within these complexes to accomplish its biological functions effectively.
- Pathways
HABP2 integrates into the fibrinolytic and coagulation pathways. It contributes to ensuring that the balance between clot formation and breakdown is maintained. The protein shows interactions with other components like urokinase-type plasminogen activator (uPA) which also plays a role in fibrinolysis. This highlights HABP2's participation in processes that involve preventing thrombosis.
- Associated diseases and disorders
HABP2 has associations with cardiovascular disorders including venous thromboembolism. Aberrant activity of HABP2 may contribute to an increased risk of thrombosis. The protein's interaction with plasminogen and fibrin suggests its involvement in abnormal clotting disorders. Further study into HABP2's role could aid in understanding its potential as a therapeutic target in these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-HABP2 antibody [EPR14551] - BSA and Azide free (ab250514), expandable thumbnail](https://content.abcam.com/products/images/habp2-antibody-epr14551-bsa-and-azide-free-ab250514--western-blot-img144992.jpg "Western blot - Anti-HABP2 antibody [EPR14551] - BSA and Azide free (ab250514)")
Western blot - Anti-HABP2 antibody [EPR14551] - BSA and Azide free (ab250514)
All lanes: Western blot - Anti-HABP2 antibody [EPR14551] (Anti-HABP2 antibody [EPR14551] ab181837) at 1/10000 dilution
Lane 1: MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: A549 (Human lung carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 3: Mouse brain lysate at 20 µg
Lane 4: Mouse heart lysate at 20 µg
Lane 5: Rat brain lysate at 20 µg
Lane 6: Rat heart lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/20000 dilution
Predicted band size: 63 kDa
Observed band size: 75 kDa
![Flow Cytometry (Intracellular) - Anti-HABP2 antibody [EPR14551] - BSA and Azide free (ab250514), expandable thumbnail](https://content.abcam.com/products/images/habp2-antibody-epr14551-bsa-and-azide-free-ab250514--flow-cytometry-intracellular-img80186.jpg "Flow Cytometry (Intracellular) - Anti-HABP2 antibody [EPR14551] - BSA and Azide free (ab250514)")
Flow Cytometry (Intracellular) - Anti-HABP2 antibody [EPR14551] - BSA and Azide free (ab250514)
This data was developed using Anti-HABP2 antibody [EPR14551] ab181837, the same antibody clone in a different buffer formulation. Intracellular Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling HABP2 with purified Anti-HABP2 antibody [EPR14551] ab181837 at 1/20 dilution (10 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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