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AB237045

Anti-HACE1 antibody

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(2 Publications)

Rabbit Polyclonal HACE1 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HACE1 aa 300-450.

View Alternative Names

KIAA1320, HACE1, E3 ubiquitin-protein ligase HACE1, HECT domain and ankyrin repeat-containing E3 ubiquitin-protein ligase 1, HECT-type E3 ubiquitin transferase HACE1

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HACE1 antibody (AB237045)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HACE1 antibody (AB237045)

Paraffin-embedded human heart tissue stained for HACE1 using ab237045 at 1/600 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HACE1 antibody (AB237045)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HACE1 antibody (AB237045)

Paraffin-embedded human brain tissue stained for HACE1 using ab237045 at 1/600 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human HACE1 aa 300-450. The exact immunogen used to generate this antibody is proprietary information.

Q8IYU2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HACE1 also known as HECT domain and ankyrin repeat-containing E3 ubiquitin-protein ligase 1 is a protein with a mass of approximately 111 kDa. This protein functions as an E3 ubiquitin ligase facilitating the attachment of ubiquitin molecules to specific protein substrates marking them for degradation via the proteasome pathway. It achieves this by recognizing and binding to substrates through its ankyrin repeat domains and catalyzing the conjugation process using its HECT domain. HACE1 is ubiquitously expressed in various tissues including the brain kidney and heart indicating its broad cellular importance.
Biological function summary

HACE1 plays a role in regulating cellular processes such as apoptosis cell cycle and oxidative stress response contributing to normal cellular homeostasis. It impacts signaling pathways by tagging proteins involved in these processes for degradation ensuring proper cell function and survival. HACE1 functions as part of a ubiquitin-proteasome complex that finely tunes cell signaling cascades through selective protein turnover. Its activity helps maintain a balanced level of critical proteins within the cell influencing cellular proliferation and survival.

Pathways

Several important cellular processes involve HACE1 including the oxidative stress response and cell cycle regulation pathways. Within these pathways HACE1 interacts with proteins like RAC1 a small GTPase associated with ROS production and cellular growth control. By modulating RAC1 and other related proteins HACE1 contributes to maintaining the redox balance and ensuring proper cell cycle progression. This interaction highlights HACE1's role in safeguarding cellular integrity under variable conditions.

HACE1 has been linked to Wilms' tumor and neurodegenerative diseases. In cancer the loss of HACE1 function results in unchecked cellular proliferation and tumor development marking it as a potential tumor suppressor. The protein's downregulation or mutation is often observed in Wilms' tumor where it interacts with pathways involving TP53 another significant tumor suppressor. In the context of neurodegenerative diseases HACE1's role in managing oxidative stress suggests a connection with disorders such as Parkinson's disease where oxidative damage is a critical pathogenic factor.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

E3 ubiquitin-protein ligase involved in Golgi membrane fusion and regulation of small GTPases (PubMed : 15254018, PubMed : 21988917, PubMed : 22036506, PubMed : 37537642, PubMed : 38332367). Acts as a regulator of Golgi membrane dynamics during the cell cycle : recruited to Golgi membrane by Rab proteins and regulates postmitotic Golgi membrane fusion (PubMed : 21988917). Acts by mediating ubiquitination during mitotic Golgi disassembly, ubiquitination serving as a signal for Golgi reassembly later, after cell division (PubMed : 21988917). Specifically binds GTP-bound RAC1, mediating ubiquitination and subsequent degradation of active RAC1, thereby playing a role in host defense against pathogens (PubMed : 22036506, PubMed : 37537642, PubMed : 38332367). May also act as a transcription regulator via its interaction with RARB (By similarity).
See full target information HACE1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of Cancer 15:3114-3127 PubMed38706891

2024

Inhibition of esophageal cancer progression through HACE1-TRIP12 interaction and associated RAC1 ubiquitination and degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Ya Hu,Ziyi Zhu,Yanhua Xu,Muhammad Fakhar Zaman,Yuxuan Ge,Jinming Hu,Xi Tang

Neoplasia (New York, N.Y.) 32:100825 PubMed35901621

2022

Longitudinal examination of perfusion and angiogenesis markers in primary colorectal tumors shows distinct signatures for metronomic and maximum-tolerated dose strategies.

Applications

Unspecified application

Species

Unspecified reactive species

Ariel I Mundo,Abdussaboor Muhammad,Kerlin Balza,Christopher E Nelson,Timothy J Muldoon
View all publications
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