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AB133637

Anti-HACE1 antibody [EPR7962]

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(11 Publications)

Rabbit Recombinant Monoclonal HACE1 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 11 publications.

View Alternative Names

KIAA1320, HACE1, E3 ubiquitin-protein ligase HACE1, HECT domain and ankyrin repeat-containing E3 ubiquitin-protein ligase 1, HECT-type E3 ubiquitin transferase HACE1

4 Images
Western blot - Anti-HACE1 antibody [EPR7962] (AB133637)
  • WB

Lab

Western blot - Anti-HACE1 antibody [EPR7962] (AB133637)

Blocking buffer : 5% NFDM/TBST
Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-HACE1 antibody [EPR7962] (ab133637) at 1/1000 dilution

Lane 1:

HEK293 whole cell lysate at 20 µg

Lane 2:

SH-SY5Y whole cell lysate at 20 µg

Lane 3:

Human fetal brain tissue lysate at 20 µg

Secondary

All lanes:

Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 102 kDa

Observed band size: 102 kDa

false

Western blot - Anti-HACE1 antibody [EPR7962] (AB133637)
  • WB

Unknown

Western blot - Anti-HACE1 antibody [EPR7962] (AB133637)

All lanes:

Western blot - Anti-HACE1 antibody [EPR7962] (ab133637) at 1/1000 dilution

Lane 1:

293T cell lysate

Lane 2:

SH-SY5Y cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 102 kDa

false

Western blot - Anti-HACE1 antibody [EPR7962] (AB133637)
  • WB

Lab

Western blot - Anti-HACE1 antibody [EPR7962] (AB133637)

Blocking buffer : 5% NFDM/TBST
Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-HACE1 antibody [EPR7962] (ab133637) at 1/5000 dilution

All lanes:

Mouse brain tissue lysate at 20 µg

Secondary

All lanes:

Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 102 kDa

Observed band size: 102 kDa

false

Western blot - Anti-HACE1 antibody [EPR7962] (AB133637)
  • WB

Lab

Western blot - Anti-HACE1 antibody [EPR7962] (AB133637)

Blocking buffer : 5% NFDM/TBST
Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-HACE1 antibody [EPR7962] (ab133637) at 1/5000 dilution

All lanes:

Rat brain tissue lysate at 20 µg

Secondary

All lanes:

Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 102 kDa

Observed band size: 102 kDa

false

  • Carrier free

    Anti-HACE1 antibody [EPR7962] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7962

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HACE1 also known as HECT domain and ankyrin repeat-containing E3 ubiquitin-protein ligase 1 is a protein with a mass of approximately 111 kDa. This protein functions as an E3 ubiquitin ligase facilitating the attachment of ubiquitin molecules to specific protein substrates marking them for degradation via the proteasome pathway. It achieves this by recognizing and binding to substrates through its ankyrin repeat domains and catalyzing the conjugation process using its HECT domain. HACE1 is ubiquitously expressed in various tissues including the brain kidney and heart indicating its broad cellular importance.
Biological function summary

HACE1 plays a role in regulating cellular processes such as apoptosis cell cycle and oxidative stress response contributing to normal cellular homeostasis. It impacts signaling pathways by tagging proteins involved in these processes for degradation ensuring proper cell function and survival. HACE1 functions as part of a ubiquitin-proteasome complex that finely tunes cell signaling cascades through selective protein turnover. Its activity helps maintain a balanced level of critical proteins within the cell influencing cellular proliferation and survival.

Pathways

Several important cellular processes involve HACE1 including the oxidative stress response and cell cycle regulation pathways. Within these pathways HACE1 interacts with proteins like RAC1 a small GTPase associated with ROS production and cellular growth control. By modulating RAC1 and other related proteins HACE1 contributes to maintaining the redox balance and ensuring proper cell cycle progression. This interaction highlights HACE1's role in safeguarding cellular integrity under variable conditions.

HACE1 has been linked to Wilms' tumor and neurodegenerative diseases. In cancer the loss of HACE1 function results in unchecked cellular proliferation and tumor development marking it as a potential tumor suppressor. The protein's downregulation or mutation is often observed in Wilms' tumor where it interacts with pathways involving TP53 another significant tumor suppressor. In the context of neurodegenerative diseases HACE1's role in managing oxidative stress suggests a connection with disorders such as Parkinson’s disease where oxidative damage is a critical pathogenic factor.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

E3 ubiquitin-protein ligase involved in Golgi membrane fusion and regulation of small GTPases (PubMed : 15254018, PubMed : 21988917, PubMed : 22036506, PubMed : 37537642, PubMed : 38332367). Acts as a regulator of Golgi membrane dynamics during the cell cycle : recruited to Golgi membrane by Rab proteins and regulates postmitotic Golgi membrane fusion (PubMed : 21988917). Acts by mediating ubiquitination during mitotic Golgi disassembly, ubiquitination serving as a signal for Golgi reassembly later, after cell division (PubMed : 21988917). Specifically binds GTP-bound RAC1, mediating ubiquitination and subsequent degradation of active RAC1, thereby playing a role in host defense against pathogens (PubMed : 22036506, PubMed : 37537642, PubMed : 38332367). May also act as a transcription regulator via its interaction with RARB (By similarity).
See full target information HACE1
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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Nature structural & molecular biology 31:364-377 PubMed38332367

2024

Structural mechanisms of autoinhibition and substrate recognition by the ubiquitin ligase HACE1.

Applications

Unspecified application

Species

Unspecified reactive species

Jonas Düring,Madita Wolter,Julia J Toplak,Camilo Torres,Olexandr Dybkov,Thornton J Fokkens,Katherine E Bohnsack,Henning Urlaub,Wieland Steinchen,Christian Dienemann,Sonja Lorenz

Hepatology communications 8: PubMed38285876

2024

Cellular origin and molecular mechanisms of lung metastases in patients with aggressive hepatoblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ruhi Gulati,Maggie Lutz,Margaret Hanlon,Ashley Cast,Rebekah Karns,James Geller,Alex Bondoc,Gregory Tiao,Lubov Timchenko,Nikolai A Timchenko

Clinical and translational medicine 12:e770 PubMed35343092

2022

Ubiquitylation of cyclin C by HACE1 regulates cisplatin-associated sensitivity in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Hong-Yue Jiang,Ying-Ling Chen,Xing-Xing Xu,Chuan-Yin Li,Yun Chen,Dong-Ping Li,Xiao-Qing Zeng,Hong Gao

FEBS letters 595:491-506 PubMed33421097

2021

Ubiquitin-independent proteasomal degradation of Spindlin-1 by the E3 ligase HACE1 contributes to cell-cell adhesion.

Applications

Unspecified application

Species

Unspecified reactive species

Vivek Reddy Palicharla,Devanshi Gupta,Debjani Bhattacharya,Subbareddy Maddika

Oncology letters 20:280 PubMed33014158

2020

Demethylation of gene promoter by propofol promotes autophagy of human A549 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shanshan Li,Hui Yang,Min Zhao,Linli Gong,Yahong Wang,Zhiyong Lv,Yuhang Quan,Zhonghui Wang

Molecular cancer therapeutics 19:1462-1473 PubMed32371578

2020

Rac Signaling Drives Clear Cell Renal Carcinoma Tumor Growth by Priming the Tumor Microenvironment for an Angiogenic Switch.

Applications

Unspecified application

Species

Unspecified reactive species

Erik T Goka,Pallavi Chaturvedi,Dayrelis T Mesa Lopez,Marc E Lippman

Cancer research 80:3009-3022 PubMed32366477

2020

HACE1 Prevents Lung Carcinogenesis via Inhibition of RAC-Family GTPases.

Applications

Unspecified application

Species

Unspecified reactive species

Melanie Kogler,Luigi Tortola,Gian Luca Negri,Alexandra Leopoldi,Amal M El-Naggar,Stefan Mereiter,Carlos Gomez-Diaz,Roberto Nitsch,Davide Tortora,Anoop M Kavirayani,Bianca V Gapp,Shuan Rao,Iris Uribesalgo,David Hoffmann,Domagoj Cikes,Maria Novatchkova,David A Williams,Jeffrey M Trent,Fumiyo Ikeda,Mads Daugaard,Astrid Hagelkruys,Poul H Sorensen,Josef M Penninger

Oncology letters 17:4361-4368 PubMed30988809

2019

Demethylation of the HACE1 gene promoter inhibits the proliferation of human liver cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zhijun Yu,Yinyin Li,Tao Han,Zhaozhe Liu

Cell death & disease 10:21 PubMed30622235

2019

HACE1 is a potential tumor suppressor in osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Amal M El-Naggar,Paul W Clarkson,Gian Luca Negri,Busra Turgu,Fan Zhang,Michael S Anglesio,Poul H Sorensen

Journal of medical genetics 52:797-803 PubMed26424145

2015

HACE1 deficiency causes an autosomal recessive neurodevelopmental syndrome.

Applications

WB

Species

Unspecified reactive species

Ronja Hollstein,David A Parry,Lisa Nalbach,Clare V Logan,Tim M Strom,Verity L Hartill,Ian M Carr,Georg C Korenke,Sandeep Uppal,Mushtaq Ahmed,Thomas Wieland,Alexander F Markham,Christopher P Bennett,Gabriele Gillessen-Kaesbach,Eamonn G Sheridan,Frank J Kaiser,David T Bonthron
View all publications
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Product promise

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