AB110284

Anti-HADH antibody [1A12BC8]

Name: Anti-HADH antibody [1A12BC8] (ab110284) | Abcam
Brand: Abcam Limited
SKU: AB110284
Price: 485 USD
Availability: InStock

Be the first to review this product! Submit a review

(3 Publications)

Mouse Monoclonal HADH antibody. Suitable for IP, Flow Cyt, IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications.

View Alternative Names

HAD, HAD1, HADHSC, SCHAD, HADH, HCDH, Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase, Short-chain 3-hydroxyacyl-CoA dehydrogenase

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-HADH antibody [1A12BC8] (AB110284)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-HADH antibody [1A12BC8] (AB110284)

MRC5 fibroblasts labeled with ab110284 at 5μg/ml and stained with Texas Red conjugated to goat anti-mouse secondary.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HADH antibody [1A12BC8] (AB110284)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HADH antibody [1A12BC8] (AB110284)

ab110284, at 1/250 dilution, staining HADH in formalin-fixed, paraffin-embedded Human cerebellum by Immunohistochemistry. HADH immunoactivity is most intense in neuronal cell bodies, most notably in the large Purkinje cells.

Flow Cytometry - Anti-HADH antibody [1A12BC8] (AB110284)

Flow Cyt

Unknown

Flow Cytometry - Anti-HADH antibody [1A12BC8] (AB110284)

HL-60 cells were stained with 1 µg/ml ab110284 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.

Unknown

Immunoprecipitation - Anti-HADH antibody [1A12BC8] (AB110284)

HADH immunocaptured from Human liver mitochondria using ab110284.

All lanes:

Immunoprecipitation - Anti-HADH antibody [1A12BC8] (ab110284)

Predicted band size: 34 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

1A12BC8

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF, IP, Flow Cyt

applications

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg/mL", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250", "IHCP-species-notes": "Perform heat mediated antigen retrieval - 1 min pressure cook in 1mmol EDTA pH8.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "" } } }

style

left-column

Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Proprietary technique

Purification notes

Purity near homogeneity as judged by SDS-PAGE (purity >95%). The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.

Storage buffer

pH: 7.5 Preservative: 0.02% Sodium azide Constituents: HEPES buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The HADH protein also known as 3-hydroxyacyl-CoA dehydrogenase facilitates the penultimate step in the beta-oxidation of fatty acids. It catalyzes the conversion of 3-hydroxyacyl-CoA to 3-ketoacyl-CoA. This enzyme exhibits a molecular mass of about 34 kDa. HADH is expressed predominantly in the liver kidney heart and muscle tissues reflecting its role in energy metabolism.

Biological function summary

The HADH enzyme holds significant responsibility for mitochondrial fatty acid oxidation. It plays a role in the degradation of medium and short-chain fatty acids. As part of a larger enzyme complex it works integrally with other dehydrogenases to maintain cellular energy levels and metabolic homeostasis especially during fasting or periods of increased energy demand.

Pathways

HADH functions prominently in the beta-oxidation pathway of fatty acid metabolism. This enzyme works in conjunction with acyl-CoA dehydrogenases further highlighting its role in breaking down fatty acids into acetyl-CoA an important molecule for energy production in the citric acid cycle. The activity of HADH interacts closely with other mitochondrial enzymes to maintain efficient energy turnover.

Mutations in the HADH gene contribute significantly to diseases such as hyperinsulinism and mitochondrial trifunctional protein deficiency. These conditions showcase the importance of HADH in metabolic regulation and energy balance. HADH-related dysfunctions also connect with enzymes like short-chain 3-hydroxyacyl-CoA dehydrogenase evidencing its role in broader metabolic disease contexts.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Mitochondrial fatty acid beta-oxidation enzyme that catalyzes the third step of the beta-oxidation cycle for medium and short-chain 3-hydroxy fatty acyl-CoAs (C4 to C10) (PubMed : 10231530, PubMed : 11489939, PubMed : 16725361). Plays a role in the control of insulin secretion by inhibiting the activation of glutamate dehydrogenase 1 (GLUD1), an enzyme that has an important role in regulating amino acid-induced insulin secretion (By similarity). Plays a role in the maintenance of normal spermatogenesis through the reduction of fatty acid accumulation in the testes (By similarity).

See full target information HADH

style

left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 14:1324205 PubMed38090559

2023

Lipid metabolism-related gene expression in the immune microenvironment predicts prognostic outcomes in renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Zhang,Bingbiao Lin,Huikun Chen,Yinyan Ye,Yijie Huang,Zhen Chen,Jun Li

Molecular medicine reports 27: PubMed36562355

2022

DCXR promotes cell proliferation by promoting the activity of aerobic glycolysis in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yongmei Jin,Miao Zhang,Yang Tong,Lin Qiu,Ying Ye,Bin Zhao

Cell death & disease 13:912 PubMed36309482

2022

Bromodomain-containing protein 4 (BRD4) as an epigenetic regulator of fatty acid metabolism genes and ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Minghua Yang,Ke Liu,Pan Chen,Hongyi Zhu,Junjie Wang,Jun Huang

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HADH antibody [1A12BC8]

Immunocytochemistry/ Immunofluorescence - Anti-HADH antibody [1A12BC8] (AB110284)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HADH antibody [1A12BC8] (AB110284)

Flow Cytometry - Anti-HADH antibody [1A12BC8] (AB110284)

Immunoprecipitation - Anti-HADH antibody [1A12BC8] (AB110284)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise