JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB154088

Anti-HADH antibody

5

(3 Reviews)

|

(15 Publications)

Rabbit Polyclonal HADH antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 15 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HADH aa 50 to C-terminus.

View Alternative Names

HAD, HAD1, HADHSC, SCHAD, HADH, HCDH, Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase, Short-chain 3-hydroxyacyl-CoA dehydrogenase

7 Images
Immunocytochemistry/ Immunofluorescence - Anti-HADH antibody (AB154088)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-HADH antibody (AB154088)

Immunofluorescence analysis of HeLa cells (fixed with 2% paraformaldehyde/culture medium at 37 °C for 30 min) labeling HADH with ab154088 at 1/500 dilution (green). The image in the right panel is costained with Hoechst 33342 (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HADH antibody (AB154088)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HADH antibody (AB154088)

Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling HADH with ab154088 at 1/250 dilution.

Western blot - Anti-HADH antibody (AB154088)
  • WB

Unknown

Western blot - Anti-HADH antibody (AB154088)

10% SDS-PAGE

All lanes:

Western blot - Anti-HADH antibody (ab154088) at 1/1000 dilution

Lane 1:

HEK-293T whole cell lysate at 30 µg

Lane 2:

A431 whole cell lysate/extract at 30 µg

Lane 3:

HeLa whole cell lysate/extract at 30 µg

Lane 4:

HepG2 whole cell lysate/extract at 30 µg

Predicted band size: 34 kDa

false

Western blot - Anti-HADH antibody (AB154088)
  • WB

Unknown

Western blot - Anti-HADH antibody (AB154088)

10% SDS PAGE

All lanes:

Western blot - Anti-HADH antibody (ab154088) at 1/1000 dilution

All lanes:

mouse liver whole cell lysate at 50 µg

Predicted band size: 29 kDa,34 kDa

Observed band size: 45 kDa

false

Western blot - Anti-HADH antibody (AB154088)
  • WB

Supplier Data

Western blot - Anti-HADH antibody (AB154088)

12% SDS-PAGE

All lanes:

Western blot - Anti-HADH antibody (ab154088) at 1/1000 dilution

All lanes:

Rat tissue extract at 50 µg

Predicted band size: 34 kDa

false

Western blot - Anti-HADH antibody (AB154088)
  • WB

Supplier Data

Western blot - Anti-HADH antibody (AB154088)

12% SDS-PAGE

All lanes:

Western blot - Anti-HADH antibody (ab154088) at 1/1000 dilution

All lanes:

Rat tissue extract at 50 µg

Predicted band size: 34 kDa

false

Western blot - Anti-HADH antibody (AB154088)
  • WB

CiteAb

Western blot - Anti-HADH antibody (AB154088)

HADH western blot using anti-HADH antibody ab154088. Publication image and figure legend from Skattebo, Ø., Bjerring, A. W., et al., 2020, Eur J Appl Physiol, PubMed 32172291.

ab154088 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab154088 please see the product overview.

The percentage change in protein content and representative Western blots for citrate synthase (CS), cytochrome c oxidase subunit 4 (COX-IV) and hydroxyacyl-CoA dehydrogenase (HAD) from pre- to post-training. The error bars indicate standard error of the mean. Black circles indicate individual responses. *All proteins increased from pre- to post-training (p < 0.001). N = 10

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human HADH aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q16836

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The HADH protein also known as 3-hydroxyacyl-CoA dehydrogenase facilitates the penultimate step in the beta-oxidation of fatty acids. It catalyzes the conversion of 3-hydroxyacyl-CoA to 3-ketoacyl-CoA. This enzyme exhibits a molecular mass of about 34 kDa. HADH is expressed predominantly in the liver kidney heart and muscle tissues reflecting its role in energy metabolism.
Biological function summary

The HADH enzyme holds significant responsibility for mitochondrial fatty acid oxidation. It plays a role in the degradation of medium and short-chain fatty acids. As part of a larger enzyme complex it works integrally with other dehydrogenases to maintain cellular energy levels and metabolic homeostasis especially during fasting or periods of increased energy demand.

Pathways

HADH functions prominently in the beta-oxidation pathway of fatty acid metabolism. This enzyme works in conjunction with acyl-CoA dehydrogenases further highlighting its role in breaking down fatty acids into acetyl-CoA an important molecule for energy production in the citric acid cycle. The activity of HADH interacts closely with other mitochondrial enzymes to maintain efficient energy turnover.

Mutations in the HADH gene contribute significantly to diseases such as hyperinsulinism and mitochondrial trifunctional protein deficiency. These conditions showcase the importance of HADH in metabolic regulation and energy balance. HADH-related dysfunctions also connect with enzymes like short-chain 3-hydroxyacyl-CoA dehydrogenase evidencing its role in broader metabolic disease contexts.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Mitochondrial fatty acid beta-oxidation enzyme that catalyzes the third step of the beta-oxidation cycle for medium and short-chain 3-hydroxy fatty acyl-CoAs (C4 to C10) (PubMed : 10231530, PubMed : 11489939, PubMed : 16725361). Plays a role in the control of insulin secretion by inhibiting the activation of glutamate dehydrogenase 1 (GLUD1), an enzyme that has an important role in regulating amino acid-induced insulin secretion (By similarity). Plays a role in the maintenance of normal spermatogenesis through the reduction of fatty acid accumulation in the testes (By similarity).
See full target information HADH
style
left-column

Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Translational oncology 49:102112 PubMed39226735

2024

HADH suppresses clear cell renal cell carcinoma progression through reduced NRF2-dependent glutathione synthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Changbin Chu,Shangjing Liu,Zhiting He,Mingjun Wu,Jing Xia,Hongxiang Zeng,Wenhua Xie,Rui Cheng,Xueya Zhao,Xi Li

Experimental physiology 108:874-890 PubMed37184360

2023

Cardiomyocyte tetrahydrobiopterin synthesis regulates fatty acid metabolism and susceptibility to ischaemia-reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Sandy M Chu,Lisa C Heather,Surawee Chuaiphichai,Thomas Nicol,Benjamin Wright,Matthieu Miossec,Jennifer K Bendall,Gillian Douglas,Mark J Crabtree,Keith M Channon

Frontiers in physiology 13:837634 PubMed35299664

2022

No Differences Between 12 Weeks of Block- vs. Traditional-Periodized Training in Performance Adaptations in Trained Cyclists.

Applications

Unspecified application

Species

Unspecified reactive species

Nicki Winfield Almquist,Hanne Berg Eriksen,Malene Wilhelmsen,Håvard Hamarsland,Steven Ing,Stian Ellefsen,Øyvind Sandbakk,Bent R Rønnestad,Knut Skovereng

American journal of translational research 13:12694-12703 PubMed34956484

2021

MiR-103a-3p aggravates renal cell carcinoma by targeting TMEM33.

Applications

Unspecified application

Species

Unspecified reactive species

Jingyu Zhang,Qingbo Lu,Haigang Pang,Min Zhang,Wenhai Wei

Medicine and science in sports and exercise 53:2333-2345 PubMed34081058

2021

Effects of Including Sprints in LIT Sessions during a 14-d Camp on Muscle Biology and Performance Measures in Elite Cyclists.

Applications

Unspecified application

Species

Unspecified reactive species

Nicki Winfield Almquist,Malene Wilhelmsen,Stian Ellefsen,Øyvind Sandbakk,Bent R Rønnestad

Diabetologia 64:1642-1659 PubMed33770195

2021

Exercise training improves mitochondrial respiration and is associated with an altered intramuscular phospholipid signature in women with obesity.

Applications

Unspecified application

Species

Unspecified reactive species

Amy E Mendham,Julia H Goedecke,Yingxu Zeng,Steen Larsen,Cindy George,Jon Hauksson,Melony C Fortuin-de Smidt,Alexander V Chibalin,Tommy Olsson,Elin Chorell

Metabolism: clinical and experimental 114:154416 PubMed33137378

2020

Pioglitazone corrects dysregulation of skeletal muscle mitochondrial proteins involved in ATP synthesis in type 2 diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Teresa Vanessa Fiorentino,Adriana Monroy,Subash Kamath,Rosa Sotero,Michele Dei Cas,Giuseppe Daniele,Alberto O Chavez,Muhammad Abdul-Ghani,Marta Letizia Hribal,Giorgio Sesti,Devjit Tripathy,Ralph A DeFronzo,Franco Folli

American journal of physiology. Endocrinology and 319:E345-E353 PubMed32543943

2020

Acute insulin deprivation results in altered mitochondrial substrate sensitivity conducive to greater fatty acid transport.

Applications

Unspecified application

Species

Unspecified reactive species

Paula M Miotto,Heather L Petrick,Graham P Holloway

Scandinavian journal of medicine & science in sports 30:1615-1631 PubMed32403173

2020

Increased oxygen extraction and mitochondrial protein expression after small muscle mass endurance training.

Applications

Unspecified application

Species

Unspecified reactive species

Øyvind Skattebo,Carlo Capelli,Bjarne Rud,Marius Auensen,Jose A L Calbet,Jostein Hallén

European journal of applied physiology 120:985-999 PubMed32172291

2020

Blood volume expansion does not explain the increase in peak oxygen uptake induced by 10 weeks of endurance training.

Applications

Unspecified application

Species

Unspecified reactive species

Øyvind Skattebo,Anders Wold Bjerring,Marius Auensen,Sebastian Imre Sarvari,Kristoffer Toldnes Cumming,Carlo Capelli,Jostein Hallén
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB110284

Anti-HADH antibody [1A12BC8]

Immunocytochemistry/ Immunofluorescence - Anti-HADH antibody [1A12BC8] (AB110284)

  • 0
  • 0

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB233838

Anti-Citrate synthetase antibody [EPR8067] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Citrate synthetase antibody [EPR8067] - BSA and Azide free (AB233838)

  • 5
  • 4
Primary Antibodies

AB110296

Anti-ACADM/MCAD antibody [3B7BH7]

Immunocytochemistry/ Immunofluorescence - Anti-ACADM/MCAD antibody [3B7BH7] (AB110296)

  • 4
  • 1
Primary Antibodies

AB156571

Anti-ACADS/SCAD antibody [EPR10862(B)]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADS/SCAD antibody [EPR10862(B)] (AB156571)

  • 5
  • 2

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com