Rabbit Polyclonal HADH antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 12 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HADH aa 50 to C-terminus.
Images
Publications
Filter by
- Frontiers in physiology 13:8376342022No Differences Between 12 Weeks of Block- vs. Traditional-Periodized Training in Performance Adaptations in Trained Cyclists.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNicki Winfield Almquist,Hanne Berg Eriksen,Malene Wilhelmsen,Håvard Hamarsland,Steven Ing,Stian Ellefsen,Øyvind Sandbakk,Bent R Rønnestad,Knut SkoverengPubMed 35299664
- American journal of translational research 13:12694-127032021MiR-103a-3p aggravates renal cell carcinoma by targeting TMEM33.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJingyu Zhang,Qingbo Lu,Haigang Pang,Min Zhang,Wenhai WeiPubMed 34956484
- Medicine and science in sports and exercise 53:2333-23452021Effects of Including Sprints in LIT Sessions during a 14-d Camp on Muscle Biology and Performance Measures in Elite Cyclists.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNicki Winfield Almquist,Malene Wilhelmsen,Stian Ellefsen,Øyvind Sandbakk,Bent R RønnestadPubMed 34081058
- Diabetologia 64:1642-16592021Exercise training improves mitochondrial respiration and is associated with an altered intramuscular phospholipid signature in women with obesity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAmy E Mendham,Julia H Goedecke,Yingxu Zeng,Steen Larsen,Cindy George,Jon Hauksson,Melony C Fortuin-de Smidt,Alexander V Chibalin,Tommy Olsson,Elin ChorellPubMed 33770195
- Metabolism: clinical and experimental 114:1544162020Pioglitazone corrects dysregulation of skeletal muscle mitochondrial proteins involved in ATP synthesis in type 2 diabetes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTeresa Vanessa Fiorentino,Adriana Monroy,Subash Kamath,Rosa Sotero,Michele Dei Cas,Giuseppe Daniele,Alberto O Chavez,Muhammad Abdul-Ghani,Marta Letizia Hribal,Giorgio Sesti,Devjit Tripathy,Ralph A DeFronzo,Franco FolliPubMed 33137378
- American journal of physiology. Endocrinology and 319:E345-E3532020Acute insulin deprivation results in altered mitochondrial substrate sensitivity conducive to greater fatty acid transport.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPaula M Miotto et. al.PubMed 32543943
- Scandinavian journal of medicine & science in sports 30:1615-16312020Increased oxygen extraction and mitochondrial protein expression after small muscle mass endurance training.Applications:Unspecified applicationReactive species:Unspecified reactive speciesØyvind Skattebo,Carlo Capelli,Bjarne Rud,Marius Auensen,Jose A L Calbet,Jostein HallénPubMed 32403173
- European journal of applied physiology 120:985-9992020Blood volume expansion does not explain the increase in peak oxygen uptake induced by 10 weeks of endurance training.Applications:Unspecified applicationReactive species:Unspecified reactive speciesØyvind Skattebo et. al.PubMed 32172291
- Scandinavian journal of medicine & science in sports 29:1866-18802019Acute and sustained effects of a periodized carbohydrate intake using the sleep-low model in endurance-trained males.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSimon Riis,Andreas Buch Møller,Ole Dollerup,Line Høffner,Niels Jessen,Klavs MadsenPubMed 31430404
- American journal of physiology. Regulatory, integr 314:R909-R9152018Sex differences in mitochondrial respiratory function in human skeletal muscle.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPaula M Miotto et. al.PubMed 29513564
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human HADH aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q16836
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected |
| Rat | Expected | Tested | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Rat | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Mitochondrial fatty acid beta-oxidation enzyme that catalyzes the third step of the beta-oxidation cycle for medium and short-chain 3-hydroxy fatty acyl-CoAs (C4 to C10) (PubMed:10231530, PubMed:11489939, PubMed:16725361). Plays a role in the control of insulin secretion by inhibiting the activation of glutamate dehydrogenase 1 (GLUD1), an enzyme that has an important role in regulating amino acid-induced insulin secretion (By similarity). Plays a role in the maintenance of normal spermatogenesis through the reduction of fatty acid accumulation in the testes (By similarity).
Alternative names
HAD, HAD1, HADHSC, SCHAD, HADH, HCDH, Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase, Short-chain 3-hydroxyacyl-CoA dehydrogenase
Recommended products
Rabbit Polyclonal HADH antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 12 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HADH aa 50 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human HADH aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q16836
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The HADH protein also known as 3-hydroxyacyl-CoA dehydrogenase facilitates the penultimate step in the beta-oxidation of fatty acids. It catalyzes the conversion of 3-hydroxyacyl-CoA to 3-ketoacyl-CoA. This enzyme exhibits a molecular mass of about 34 kDa. HADH is expressed predominantly in the liver kidney heart and muscle tissues reflecting its role in energy metabolism.
- Biological function summary
The HADH enzyme holds significant responsibility for mitochondrial fatty acid oxidation. It plays a role in the degradation of medium and short-chain fatty acids. As part of a larger enzyme complex it works integrally with other dehydrogenases to maintain cellular energy levels and metabolic homeostasis especially during fasting or periods of increased energy demand.
- Pathways
HADH functions prominently in the beta-oxidation pathway of fatty acid metabolism. This enzyme works in conjunction with acyl-CoA dehydrogenases further highlighting its role in breaking down fatty acids into acetyl-CoA an important molecule for energy production in the citric acid cycle. The activity of HADH interacts closely with other mitochondrial enzymes to maintain efficient energy turnover.
- Associated diseases and disorders
Mutations in the HADH gene contribute significantly to diseases such as hyperinsulinism and mitochondrial trifunctional protein deficiency. These conditions showcase the importance of HADH in metabolic regulation and energy balance. HADH-related dysfunctions also connect with enzymes like short-chain 3-hydroxyacyl-CoA dehydrogenase evidencing its role in broader metabolic disease contexts.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
Western blot - Anti-HADH antibody (ab154088)
10% SDS PAGE
All lanes: Western blot - Anti-HADH antibody (ab154088) at 1/1000 dilution
All lanes: mouse liver whole cell lysate at 50 µg
Predicted band size: 29 kDa, 34 kDa
Observed band size: 45 kDa
Western blot - Anti-HADH antibody (ab154088)
10% SDS-PAGE
All lanes: Western blot - Anti-HADH antibody (ab154088) at 1/1000 dilution
Lane 1: HEK-293T whole cell lysate at 30 µg
Lane 2: A431 whole cell lysate/extract at 30 µg
Lane 3: HeLa whole cell lysate/extract at 30 µg
Lane 4: HepG2 whole cell lysate/extract at 30 µg
Predicted band size: 34 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HADH antibody (ab154088)
Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling HADH with ab154088 at 1/250 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-HADH antibody (ab154088)
Immunofluorescence analysis of HeLa cells (fixed with 2% paraformaldehyde/culture medium at 37 °C for 30 min) labeling HADH with ab154088 at 1/500 dilution (green). The image in the right panel is costained with Hoechst 33342 (blue).
Western blot - Anti-HADH antibody (ab154088)
12% SDS-PAGE
All lanes: Western blot - Anti-HADH antibody (ab154088) at 1/1000 dilution
All lanes: Rat tissue extract at 50 µg
Predicted band size: 34 kDa
Western blot - Anti-HADH antibody (ab154088)
12% SDS-PAGE
All lanes: Western blot - Anti-HADH antibody (ab154088) at 1/1000 dilution
All lanes: Rat tissue extract at 50 µg
Predicted band size: 34 kDa
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-HADH antibody (ab154088)
HADH western blot using anti-HADH antibody ab154088. Publication image and figure legend from Skattebo, Ø., Bjerring, A. W., et al., 2020, Eur J Appl Physiol, PubMed 32172291.
ab154088 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab154088 please see the product overview.
The percentage change in protein content and representative Western blots for citrate synthase (CS), cytochrome c oxidase subunit 4 (COX-IV) and hydroxyacyl-CoA dehydrogenase (HAD) from pre- to post-training. The error bars indicate standard error of the mean. Black circles indicate individual responses. *All proteins increased from pre- to post-training (P < 0.001). N = 10
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com