Anti-HADHA antibody

• WB

Supplier Data

Western blot - Anti-HADHA antibody (AB54477)

All lanes:

Western blot - Anti-HADHA antibody (ab54477) at 4 µg/mL

Lanes 1 - 2:

Jurkat cell lysate 30-50 ug/lane

Lane 3:

3T3 cell lysate 30-50 ug/lane

Lane 4:

Rat kidney lysate 30-50 ug/lane

Secondary

All lanes:

Anti-Rabbit IgG, HRP-Linked Antibody at 1/5000 dilution

Predicted band size: 83 kDa

Observed band size: 83 kDa

false

• WB

CiteAb

Western blot - Anti-HADHA antibody (AB54477)

HADHA western blot using anti-HADHA antibody ab54477. Publication image and figure legend from Miklas, J. W., Clark, E., et al., 2019, Nat Commun, PubMed 31604922.

ab54477 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab54477 please see the product overview.

Generation of HADHA Mutant and Knockout stem cell derived cardiomyocytes. a Schematic of fatty acid beta-oxidation detailing the four enzymatic steps. b Schematic of HADHA KO DNA and protein sequence from WTC iPSC line showing a 22 bp deletion, which resulted in an early stop codon. c Schematic of HADHA Mut DNA and protein sequence from WTC iPSC line showing a 2 bp deletion and 9 bp insertion on the first allele and a 2 bp deletion on the second allele. RNA-Sequencing read counts show that the HADHA Mut expresses exons 4–20 resulting in a truncated protein. d Western analysis of HADHA expression and housekeeping protein β-Actin in WTC iPSCs. e Confocal microscopy of WT, HADHA Mut and HADHA KO hiPSC-CMs for the cardiac marker αActinin (green) and HADHA (red). f Seahorse analysis trace of fatty acid oxidation capacity of WT, HADHA Mut and HADHA KO hiPSC-CMs. n = 6–7 biological replicates. Source data are provided as a Source Data file

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